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بررسی بروز آبسۀ کبدی در گاوهای شیرده و خشک هلشتاین و تأثیر آن بر برخی فراسنجه‌های خونی و بافت‌شناسی یاخته‌های کبد

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، دانشکدۀ کشاورزی، دانشگاه زنجان، زنجان، ایران

2 استاد، دانشکدۀ کشاورزی، دانشگاه زنجان، زنجان، ایران

3 استاد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

4 استاد میکروبیولوژی، گروه پزشکی تشخیصی / پاتوبیولوژی، دانشگاه ایالتی کانزاس، منهتن، KS، ایالات‌متحده

چکیده

هدف از این پژوهش بررسی بروز آبسۀ کبدی در گاوهای شیری هلشتاین و تأثیر آن بر برخی فراسنجه­های خونی و بافت‌شناسی یاخته‌های کبدی بود. کبد 577 رأس گاو شیرده و خشک در زمان کشتار در دو کشتارگاه در ایران برای تشخیص آبسۀ کبدی بررسی شدند. خون‌گیری از گاوها در زمان نزدیک به کشتار انجام شد. 60  رأس گاو سالم یعنی به شمار گاوهای دارای آبسۀ کبدی، (که هیچ نوع مشکل از لحاظ کالبدگشایی و بازبینی امعاءواحشاء نداشتند) برابر با گله و شمار زایش به‌عنوان گروه شاهد در نظر گرفته شدند. بی‌درنگ پس از کشتار، کبد گاوها به چهار گروه کبد بدون آبسه امتیاز 0، کبد با یک یا دو آبسۀ کوچک یا آسیب بافتی امتیاز A-، کبد با یک یا دو آبسه به‌خوبی تشکیل‌شده امتیاز A و کبد با یک یا چند آبسۀ باز بزرگ، با یا بدون چسبندگی امتیاز A+  طبقه‌بندی شدند. بررسی کشتارگاهی نشان داد، 4/10 (60 رأس) درصد از گاوها آبسۀ کبدی داشتند (25، 7/31 و 3/43 درصد به ترتیب برایA-، A و A+). داده‌های به‌دست‌آمده از بررسی‌های بافت‌شناختی نشان داد، کبدهای آبسه دار در مقایسه با کبد گاوهای سالم، یاخته‌های هپاتوسیت نزدیک به سینوزوئیدها تغییر شکل یافته و بافت مرده (نکروزه)‌شده داشتند و شمار یاخته‌های کاپفر در کبدهای درگیر آبسه افزایش‌یافته بودند. غلظت آنزیم‌های کبدی آسپارتات آمینوترنسفراز (AST)، آلانین آمینوترنسفراز (ALT) و گاما گلوتامیل ترانسفراز (GGT) در سرم خون گاوهای دارای آبسۀ کبدی با امتیاز (اسکور)های A-، A و A+  در مقایسه با گاوهای سالم بالاتر بودند (01/0P≤)؛ درحالی‌که غلظت آنزیم کراتین­کیناز (CK) در گاوهای درگیر آبسۀ کمتر از گروه سالم بود (01/0P≤). گاوهای دارای آبسۀ کبدی با اسکورهای A-، A و A+ غلظت‌های پایین­تری از آلبومین و کلسترول سرم در مقایسه با گاوهای سالم داشتند (01/0P≤). نتایج این پژوهش نشان داد، غلظت بالاتر آنزیم‌های کبدی و غلظت پایین آلبومین و کلسترول سرم خون در گاوهای مبتلا به آبسۀ کبدی ممکن است درنتیجۀ تخریب یاخته‌های کبدی و التهاب باشد. درمجموع نتیجه‌گیری می‌شود که، برخی فراسنجه‌های خونی می‌توانند به‌عنوان شاخصی برای بررسی وضعیت آبسۀ کبدی استفاده شوند.

کلیدواژه‌ها


عنوان مقاله [English]

Investigation of the occurrence of liver abscesses in lactating and dry Holstein cows and its effect on some blood parameters and histology of hepatocytes

نویسندگان [English]

  • Shahrzad Joz-Ghasemi 1
  • Hamid Amanlou 2
  • Mehdi Dehghan Banadaki 3
  • T.G. Nagaraja 4
1 Ph. D. Candidate, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
2 Professor, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
3 Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
4 Professor, University Distinguished Professor of Microbiology Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS, United States
چکیده [English]

The aim of the present study was to evaluate the incidence of liver abscess in lactating and dry Holstein cows and its effect on some blood parameters and histology of hepatocytes. The liver of 577 lactating and dry Holstein cows at the time of slaughter were examined in two abattoirs in Iran. Cow’s blood samples were collected from at the time of slaughter. Sixty healthy cows, i.e., the same number of cows with liver abscesses (which had no problem in terms of autopsy and splanchnic checking), were considered as control group according to herd and number of gestation. The livers of the cows were divided into 4 groups immediately after slaughter, liver without abscess score 0, liver with one or two small abscesses or scars; score A+, liver with two to four well-organized abscesses; score A, and liver with one or more large open abscesses, with or without adhesions; score A+.  The slaughterhouse survey showed that, 10.4% (60 cows) had liver abscesses (25, 31.7 and 43.3 % for A-, A and A+). Histological results showed that in livers with abscesses, natural form of hepatocytes close to sinusoids had changed and parts of the affected tissue showed necrosis and the numbers of Kupffer cells were increased in compare to healthy livers. The concentration of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and gamma glutamyltransferase (GGT) in the blood serum of cattle with liver abscesses with A, A and A + scores were higher than that of healthy cows (P≤0.01), while the concentration of creatine kinase (CK) in the cows affected by the abscess was lower than the healthy group (P≤0.01). Cows with liver abscesses with scores A-, A and A+ had lower concentrations of albumin and serum cholesterol than healthy cows (P≤0.01). The results of this study showed that the higher concentrations of liver enzymes and low blood concentrations of albumin and cholestrol in cows with liver abscesses may be due to the destruction of liver cells and inflammation. In conclusion, some blood parameters can be used as an indicator for assessing the status of liver abscess.

کلیدواژه‌ها [English]

  • Histological studies
  • lactating cows
  • liver abscesses
  • liver enzymes
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