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بررسی چندشکلی ژن IGF-1 و ارتباط آن با صفات مرتبط با رشد در گاوهای سیستانی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی‌ارشد ژنتیک و اصلاح دام، گروه علوم دامی، دانشکدة کشاورزی، دانشگاه زابل

2 استادیار، ژنتیک و اصلاح دام، گروه علوم دامی، دانشکدة کشاورزی، دانشگاه زابل

3 دانشیار ژنتیک و اصلاح دام، گروه علوم دامی، دانشکدة کشاورزی، دانشگاه زابل

چکیده

فاکتور رشد شبه‌انسولین (IGF-1) یک هورمون پپتیدی است که ساختار مشابهی با هورمون انسولین داشته و روی کروموزوم شمارة 5 گاو قرار دارد. IGF-1 نقش مهمی در رشد و نمو بافت‌های مختلف بدن ایفا می‌کند. هدف از پژوهش حاضر، بررسی چندشکلی‌های موجود در ژن IGF-1 و تأثیر آن بر صفات مرتبط با رشد در گاوهای سیستانی است. نمونه‌های خون از ورید گردنی 53 رأس گاو سیستانی )یک‌ساله( گرفته شد و استخراج DNA به روش نمکی بهینه‌یافته از خون کامل انجام گرفت. تکثیر یک قطعه 249 جفت بازی از ناحیة اگزون 1 ژن IGF-1 توسط واکنش زنجیره‌ای پلی‌مراز (PCR) با استفاده از یک جفت آغازگر اختصاصی صورت گرفت. وجود جهش نقطه‌ای و تعیین ژنوتیپ‌های موجود در این جایگاه در هریک از نمونه‌ها با استفاده از آنزیم برشی SnaBI انجام گرفت و روی ژل آگارز 2 درصد الکتروفورز شد. نتایج از وجود دو آلل A و B در جایگاه مورد مطالعه حاکی بود که فراوانی آن‌ها به ترتیب 25/0 و 75/0 محاسبه شد. سه الگوی ژنوتیپی جایگاه مورد مطالعه شامل AA، AB و BB دارای فراوانی‌های ژنوتیپی به ترتیب برابر با 057/0، 396/0 و 547/0 بودند. آزمون مربع کای برای ناحیة اگزون 1 ژنIGF-1 در جمعیت گاو سیستانی مورد مطالعه، بیانگر برقراری تعادل هاردی-واینبرگ بود. آنالیز ارتباط ژنوتیپ‌ها در این جایگاه با داده‌های مرتبط با صفات رشد  اختلاف معناداری (05/0P<) را بین ژنوتیپ‌های AA و AB نشان داد. بر اساس نتایج تحقیق حاضر می‌توان نتیجه گرفت که ژن IGF-1 می‌تواند به‌عنوان ژن کاندیدا برای صفات مرتبط با رشد در برنامه‌های به‌نژادی گاو سیستانی استفاده شود.

کلیدواژه‌ها


عنوان مقاله [English]

Study of IGF-1 Gene Polymorphism and its association with growth related traits in Sistani cattle

نویسندگان [English]

  • Somayeh Alipoor 1
  • Gholamreza Dashab 2
  • Masoud Alipanah 3
  • Mohammad Rokouei 2
1 , M.Sc. Student, Genetic and Animal Breeding, Department of Animal Science, College of Agriculture, University of Zabol
2 Assistant Professor, Genetic and Animal Breeding, Department of Animal Science, College of Agriculture, University of Zabol
3 Associate Professor, Genetic and Animal Breeding, Department of Animal Science, College of Agriculture, University of Zabol
چکیده [English]

Insulin-Like Growth Factor-I (IGF-1) is a peptide hormone which is similar in molecular structure to insulin and it is located on chromosome 5 in cattle. Since the IGF-1 plays an important role in the growth and development of multiple tissues, the purpose of current study was to identify the IGF-1 gene polymorphism in Sistani beef cattle. A total of 53 Sistani beef cattle (one year) were randomly selected and blood samples were drawn from the cervical vein. DNA was extracted from the whole blood with modified salting-out method. A 249 bp length fragment in exon 1 of IGF-1 gene was amplified by polymorphism chain reaction by using specific primer pairs. Genotyping of each sample was performed by using restriction enzyme SnaBI and electrophoresed on 2% agarose gel. The results revealed the existence of two alleles A and B for the examined locus with the frequencies of 0.25 and 0.75, respectively. Three different genotypic variants including AA, AB and BB were identified with genotypic frequencies of 0.057, 0.396 and 0.547, respectively. Chi square test based on observed and expected frequencies of different genotypic variants of  the exon 1 of IGF-1 gene in Sistani cattle population showed that deviation from Hardy-Weinberg equilibrium was not significant. Analysis associate of the studied genotypes and growth related traits showed significant effects between AA and AB genotypes (P<0.05). On the basis of obtained results it was revealed that the IGF-1 gene can be used as a candidate gene for growth related traits in Sistani population.

کلیدواژه‌ها [English]

  • IGF1
  • IGF1/SnaBI
  • Single nucleotide polymorphism
  • Growth related traits
  • Iranian native cattle
Abdolmohammadi, A. (2008). Study of genetic polymorphism of four candidate gene with PCR-RFLP and HRM methods and its Association with production and reproductive traits in Iranian Holstein cattle, Ph.D. dissertation in genetics and Animal breeding, College of Agriculture, Tehran University, Iran. (In Farsi) 

Adam, D., Cosgrove, L. J., Booker, G.W., Wallace, J. C. & Forbes, B. E. (2005). Molecular interactions of the IGF system. Cytokine & Growth Factor Reviews, 16 (4-5), 421-43.

Andrade, P. C., Grossi, D. A., Paz, C. C., Alencar, M. M., Regitano, L. C & Munari, D. P. (2008). Association of an insulin-like growth factor 1 gene microsatellite with phenotypic variation and estimated breeding values of growth traits in Canchim cattle. Animal Genetics, 39, 480-485.

Casas, E., Shackerford, S. D., Keele, J. W., Stone, R. T., Kappes, S. M. & Koohmareie, M. (2000). Quantitative trait loci affecting growth and carcass composition of cattle segregating alternate from of myostatin. Journal of Animal Science, 78, 560-569.

Curi, R. A., Oliveira, H. N., Silveira, A. C. & Lopes, C. R. (2005). Association between IGFI, IGF-IR and GHRH gene polymorphisms and growth and carcass traits in beef cattle. Livestock Production Science, 94, 159-167.

Etherton, T. D. (2004). Somatotropic function: The somatomedin hypothesis revisited. Journal of Animal Science, 82 (E-Suppl), E239-E244.

Fotsis, T., Murphy, C. & Gannon, F. (1989). Nucleotide Sequence of the Bovine Insulin- Like Growth Factor 1 and its IGF-IA Precursor. Journal of Progress Nucleic Acid Research Molecular Biology, 18(30).

Ge, W., Davis, M. E. & Hines, H. C. (1997). Two SSCP alleles detected in the 5’-flanking region of bovine IGF1 gene. Animal Genetics, 28, 155-156.

Ge, W., Davis, M. E., Hines, H. C., Irvin, K. M. & Simmen, R. C. M. (2001). Association of genetic marker with blood serum insulin-like growth factor-I concentration and growth traits in Angus cattle. Journal of Animal Science, 79, 1757-1762.

Grossi, D. A., Grupioni, N. V., Buzanskas, M. E., Schenkel, F. S., Regitano, L. C., Paz, C. C. P., Alencar, M. M. & Munari, D. P. (2010). Association of Polymorphisms in the IGF1, GH and PIT1 Genes with Growth and Reproductive Traits in Canchim Cattle. In: Proceedings of the 9th World Congress on Genetics Applied to Livestock Production, Leipzig, Germany.

Hasanpour, K., Asgari-Moghadam, M., Bigi-Nassiri, M. T., Roshanfakr, H A., Fayazi, J. & Tabatabayei-Vakili, S. S. (2012). Study of IGF-1 gene polymorphism in hybrid cattle of Khuzestan province with PCR-RFLP method. Journal of Modern Veterinary Research, 13, 45-52. (In Farsi)

Kim, M. H., Seo, D. S. & Ko, Y. (2004). Relationship between Egg productivity and Insulin Like growth factor-1 genotypes in Korean native Ogol chickens. Poultry Science, 83, 1203-1208.

Kirkpatrick, B. W. (1992). Identification of a conserved microsatellite site in the porcine and bovine insulin-like growth factor-I gene 5’ flank. Animal Genetic, 23, 543-548.

Laureano, M. M. M., Otaviano, A. R., Lima, A. L. F., Costa, R. B., Salman, A. K. D., Sena, J. A. D., Tonhati, H. & Albuquerque, L. G. D. (2009). Characterization and polymorphism screening of IGF-1 and prolactin genes in Nelore heifers. Italian Journal of Animal Science, 8, 277-283.

Lei, M., Nie, Q., Peng, X. & Zhang, X. (2005). Single nucleotide polymorphisms of the chicken insu-like factor binding protein 2 genes associated with chicken growth and carcass traits. Poultry Science, 84, 1191-1198.

Li, C., Basarad, J., Snelling, W. M., Bwnkel, B., Murdoch, B., Hansen, C. & Moore, S. S. (2004). Assessment of positional candidate genes myf5 and igf1 for growth on bovine chromosome 5 in commercial lines of Bos taurus. Journal of  Animal  Science, 82, 1-7.

Musaro, A., McCullagh, K. J., Naya, F. J., Olsen, E. N. & Rosenthal, N. (1999). IGF-1 induces skeletal myocyte hypertrophy through calcineurin in association with GATA-2 and NF-ATc1. Nature, 400 (6744), 581-585.

Reyna, X. F., Montoya, H. M., Castrellon, V. V., Rincon, A. M., Bracamonte, M. P. & Vera, W. A. (2010). Polymorphisms in the IGF1 gene and their effect on growth traits in Mexican beef cattle. Genetics and Molecular Research, 9(2), 875-83.

Roite, D., Bondy, C., Yakar, S., Liu, J. L. & Butler, A. (2001). The Somatomedin Hypothesis. Endocrinology Review, 22, 53-74.

Sellier, P. (2000). Genetically caused retarded growth in animals. Domestic Animal Endocrinology, 19, 105-119.

Siadkowska, E., Zwierzchowski, L., Oprzadek, J. & Strzalkowska, N. (2006). Effect of polymorphism in IGF-1 gene on production traits in Polish Holstein-Friesian cattle. Animal Science. Pap. Rep., 3, 225-237.

Sirotkin, A. V., Mertin, D., Suvegova, K., Makarevich, A. V. & Mikulova, E. (2003). Effect of GH and IGF-I treatment on reproduction,growth, and plasma hormone concentrations in domestic nutria (Myocastor coypus). General and Comparative Endocrinology, 131, 296-301.

Tahmorespour, M., Vafaie-Valeh, M., Ansari, M., Heravi-Mosavi, A. R. & Nasiri, M. R (2008). Study of IGF-1 gene polymorphism and its association with daily gain in Baluchi sheep. In: The 3th Iranian Congress of Animal Science, College of Agriculture, Ferdowsi University of Mashhad. (In Farsi)

Weber, M. S. (1998). The Role of Insulin-Like Growth Factor-I (IGF-I) and IGF Binding Proteins in Mammary Gland Development, Fulfillment of the Requirements for the Degree of Doctor of Philosophy, Faculty of the Virginia Polytechnic Institute, State University.

Williams, J. L. (2008). Genetic control of meat quality traits. Meat Biotechnology.

Yazdan-Panah, A., Khedrzadeh, S. & Mohammadi-Kaftarkari, A (2010a). Study of IGF-1 gene polymorphism in Buffalo population in Khuzestan province with PBR technique. In: The 4th Iranian Congress of Animal Science, Karaj, Iran, pp. 2879-2882. (In Farsi)

Yazdan-Panah, A., Khedrzadeh, S. & Mohammadi-Kaftarkari, A. (2010b). Determination of heterozygosity in exon 1 of IGF-1 in Najdi cattle in Khuzestan province with PBR technique. In: The 4th  Iranian Congress of Animal Science, Karaj, Iran, pp. 2775-2878. (In Farsi).