بررسی چندشکلی ژن IGF-1 و ارتباط آن با صفات مرتبط با رشد در گاوهای سیستانی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی‌ارشد ژنتیک و اصلاح دام، گروه علوم دامی، دانشکدة کشاورزی، دانشگاه زابل

2 استادیار، ژنتیک و اصلاح دام، گروه علوم دامی، دانشکدة کشاورزی، دانشگاه زابل

3 دانشیار ژنتیک و اصلاح دام، گروه علوم دامی، دانشکدة کشاورزی، دانشگاه زابل

چکیده

فاکتور رشد شبه‌انسولین (IGF-1) یک هورمون پپتیدی است که ساختار مشابهی با هورمون انسولین داشته و روی کروموزوم شمارة 5 گاو قرار دارد. IGF-1 نقش مهمی در رشد و نمو بافت‌های مختلف بدن ایفا می‌کند. هدف از پژوهش حاضر، بررسی چندشکلی‌های موجود در ژن IGF-1 و تأثیر آن بر صفات مرتبط با رشد در گاوهای سیستانی است. نمونه‌های خون از ورید گردنی 53 رأس گاو سیستانی )یک‌ساله( گرفته شد و استخراج DNA به روش نمکی بهینه‌یافته از خون کامل انجام گرفت. تکثیر یک قطعه 249 جفت بازی از ناحیة اگزون 1 ژن IGF-1 توسط واکنش زنجیره‌ای پلی‌مراز (PCR) با استفاده از یک جفت آغازگر اختصاصی صورت گرفت. وجود جهش نقطه‌ای و تعیین ژنوتیپ‌های موجود در این جایگاه در هریک از نمونه‌ها با استفاده از آنزیم برشی SnaBI انجام گرفت و روی ژل آگارز 2 درصد الکتروفورز شد. نتایج از وجود دو آلل A و B در جایگاه مورد مطالعه حاکی بود که فراوانی آن‌ها به ترتیب 25/0 و 75/0 محاسبه شد. سه الگوی ژنوتیپی جایگاه مورد مطالعه شامل AA، AB و BB دارای فراوانی‌های ژنوتیپی به ترتیب برابر با 057/0، 396/0 و 547/0 بودند. آزمون مربع کای برای ناحیة اگزون 1 ژنIGF-1 در جمعیت گاو سیستانی مورد مطالعه، بیانگر برقراری تعادل هاردی-واینبرگ بود. آنالیز ارتباط ژنوتیپ‌ها در این جایگاه با داده‌های مرتبط با صفات رشد  اختلاف معناداری (05/0P<) را بین ژنوتیپ‌های AA و AB نشان داد. بر اساس نتایج تحقیق حاضر می‌توان نتیجه گرفت که ژن IGF-1 می‌تواند به‌عنوان ژن کاندیدا برای صفات مرتبط با رشد در برنامه‌های به‌نژادی گاو سیستانی استفاده شود.

کلیدواژه‌ها


عنوان مقاله [English]

Study of IGF-1 Gene Polymorphism and its association with growth related traits in Sistani cattle

نویسندگان [English]

  • Somayeh Alipoor 1
  • Gholamreza Dashab 2
  • Masoud Alipanah 3
  • Mohammad Rokouei 2
1 , M.Sc. Student, Genetic and Animal Breeding, Department of Animal Science, College of Agriculture, University of Zabol
2 Assistant Professor, Genetic and Animal Breeding, Department of Animal Science, College of Agriculture, University of Zabol
3 Associate Professor, Genetic and Animal Breeding, Department of Animal Science, College of Agriculture, University of Zabol
چکیده [English]

Insulin-Like Growth Factor-I (IGF-1) is a peptide hormone which is similar in molecular structure to insulin and it is located on chromosome 5 in cattle. Since the IGF-1 plays an important role in the growth and development of multiple tissues, the purpose of current study was to identify the IGF-1 gene polymorphism in Sistani beef cattle. A total of 53 Sistani beef cattle (one year) were randomly selected and blood samples were drawn from the cervical vein. DNA was extracted from the whole blood with modified salting-out method. A 249 bp length fragment in exon 1 of IGF-1 gene was amplified by polymorphism chain reaction by using specific primer pairs. Genotyping of each sample was performed by using restriction enzyme SnaBI and electrophoresed on 2% agarose gel. The results revealed the existence of two alleles A and B for the examined locus with the frequencies of 0.25 and 0.75, respectively. Three different genotypic variants including AA, AB and BB were identified with genotypic frequencies of 0.057, 0.396 and 0.547, respectively. Chi square test based on observed and expected frequencies of different genotypic variants of  the exon 1 of IGF-1 gene in Sistani cattle population showed that deviation from Hardy-Weinberg equilibrium was not significant. Analysis associate of the studied genotypes and growth related traits showed significant effects between AA and AB genotypes (P<0.05). On the basis of obtained results it was revealed that the IGF-1 gene can be used as a candidate gene for growth related traits in Sistani population.

کلیدواژه‌ها [English]

  • IGF1
  • IGF1/SnaBI
  • Single nucleotide polymorphism
  • Growth related traits
  • Iranian native cattle
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