نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه علوم دامی، دانشکده علوم دامی و شیلات، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران
2 گروه علوم دامی، دانشکده علوم دامی و شیلات، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
One of the goals of the sheep industry is to maintain the quality of the meat and increase the muscle mass, as well as to create welfare for the livestock. In this research, in order to investigate the effect of glutamine and protein nutrition on the expression of adipogenic genes (PPARG, LPL, CEBP/B) in the muscle of Zel sheep under heat stress conditions. Sixteen Zel male lambs (average weight 31.5 ± 0.22 kg and age 4 ± 0.5 months) were randomly selected and subjected to four experimental treatments for forty-five days. Experimental treatments include:1) Basic diet 2) Basic diet with glutamine (0.2 grams per kilogram of body weight (, 3) 10% more protein than needed, without glutamine And 4) 10% more protein with glutamine (0.2 g/kg body weight). A thigh biopsy was taken from the thigh muscle on day 42. The relative expression of genes was measured using Real Time-PCR and using specific primers of genes. The results showed that the relative expression of C/EBPB and PPARG genes increased significantly in all treatments compared to the control treatment. Also, the relative expression of LPL gene in treatment 4 (GP) was significantly decreased compared to the control treatment. Also, feeding glutamine and protein levels by changing the expression of adipogenic genes in the muscle of Zel sheep under heat stress conditions can probably have a positive effect on meat quality.
کلیدواژه‌ها [English]
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