نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه علوم دامی، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران.
2 موسسه تحقیقات علوم دامی ایران، سازمان تحقیقات و آموزش کشاورزی، کرج.
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
The aim of the present research was to isolate lactic acid-producing bacteria from pollen or bee bread. For this purpose, bacterial strains were isolated from various samples of pollen and bee bread using MRS culture medium. The characteristics of the isolates were assessed through Gram staining, catalase activity tests, fermentation of specific sugars, growth curve analysis, pH reduction in the culture medium, and gas production. The results from the growth curve analysis of the isolated bacteria demonstrated significant differences in their growth rates under identical culture conditions, indicating varying capacities for pollen fermentation. Specifically, bacteria MB1, MB2, MB4, and MP1 exhibited robust growth during the initial 48 hours. In contrast, bacteria MB3 and MP2 remained in the lag phase throughout this period; however, MP2 and MB4 subsequently experienced a marked increase in growth rate, with highest optical density (OD). Following incubation of the pollen-containing culture medium, bacterial activity in fermenting pollen was evaluated based on lactic acid production. All isolated bacteria were confirmed to be Gram-positive and catalase-negative, demonstrating the ability to ferment certain simple sugars. The isolated strains exhibited varying efficiencies in both growth rates and lactic acid production, suggesting differences in their capacity to utilize the nutrients found in pollen. However, no significant differences were observed among the bacterial strains concerning gas production.
کلیدواژهها [English]
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