Document Type : Research Paper


1 Ph.D. Candidate, Faculty of Agriculture, University of Zanjan, Zanjan, Iran

2 Professor, Faculty of Agriculture, University of Zanjan, Zanjan, Iran

3 DVM, PhD in FKA Animal Husbandry and Agriculture Co., Isfahan, Iran


The objective of this experiment was to determine the effects of fish meal supplementation on productive and reproduction performance in repeat breeder Holstein cows. Two hundred and sixty-one multiparous Holstein cows with at least three consecutive unsuccessful inseminations, without clinically detectable reproductive disorders, were randomly allocated to 1 of 3 dietary treatments. Dietary treatments were control, 2.5 or 5% fish meal, that replaced soybean meal. Cows were timed inseminated following the Double-Ovsynch protocol. Milk yield was (38.49, 39.92, and 41.42 kg/d, for cows fed the control, 2.5 and 5% FM diets respectively) of which the difference (P<0.01) between the control and 5% FM diets was significant. Likewise, 4% FCM was higher for treatment 5% FM relative to control (P<0.05). Milk fat, protein and lactose content (%) were not different among treatment (P>0.05) but milk protein yield was higher (P<0.05) for cows fed the 5% FM than control and 2.5% FM diets was significant. Plasma and milk urea nitrogen concentrations decreased significantly (P<0.05) affected fish meal by consumption. Plasma cholesterol concentration was higher for cows fed 5% FM compared to the control diet (P<0.01). Plasma progesterone concentration at 16 d post artificial insemination was greater for treatment 5% FM relative to control treatment (P<0.05). Pregnancy at the first service and cumulative (1st and 2nd service) for cows fed 5% FM content higher than control diet (P<0.05). These results indicated that supplementation the diet with 5% fish meal improved fertility and productive performance in repeat breeding cows.


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