The effect of chemical and biological regulators of pH on histomorphometry and ‎histopathology of rumen, reticulum, liver and kidney of Arabi lambs ‎

Document Type : Research Paper

Authors

1 M.Sc. Student, Department of Animal Science, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural ‎Resources, University of Khuzestan, Mollasani, Ahvaz, Iran

2 Professor, Department of Animal Science, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural ‎Resources, University of Khuzestan, Mollasani, Ahvaz, Iran

3 Ph.D. of Animal Nutrition, Kharazmi Industrial School of Dezful, Iran‎

4 Assistant Professor, Department of Veterinary Medicine, Azad University of Yasuj, Yasuj, Iran ‎

Abstract

The aim of this study was to compare the effect of rumen pH-adjusting additives on histomorphometry and histopathology of rumen, reticulum, liver, and kidney. Twelve Arabi male lambs, 9±1 months old and initial body weight of 35.95±3.55 kg were used in a completely randomized design with three treatments and four replicates in 35 days. The experimental treatments consisted of 1- A control diet, 2- Control diet+sodium bicarbonate buffer, 3- Control diet+Megasphaera elsdenii, and Saccharomyces cerevisiae (bacteria-yeast). At the end of the experiment, the lambs were slaughtered and tissue sampls from the liver, kidney, reticulum, and rumen were collected, and the tissue sections with thickness of 5 μm were prepared.  The wall thickness of the reticulum in the control treatment was significantly higher than the buffer recipient treatment (P<0.05). Also, the thickness of muscle-layer in the reticulum in the control treatment was more than other treatments and was significantly higher compare to the buffer recipient treatment (P<0.05). Papillae height and Papillae depth of reticulum in the bacteria-yeast recipient treatment were significantly higher than other treatments (P<0.05). In addition, the thickness of rumen epithelial tissue in the buffer and bacteria-yeast recipient treatments was less than the control treatment (P<0.05). The height of rumen papillae in the control treatment was significantly higher than the buffer and bacteria-yeast treatments. Infiltration of inflammatory cells into the liver, kidney and gastrointestinal tissues was observed.  In general, pH regulators, in high-concentrate diets protected the tissues, which were studied in the present experiment. In addition, the addition of bacteria-yeast effects as a biological regulator is comparable to the chemical buffer.

Keywords

References

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Iranian Journal of animal Science
Volume 52, Issue 4
February 2022
Pages 231-241

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History

  • Receive Date: 12 August 2021
  • Revise Date: 18 September 2021
  • Accept Date: 03 October 2021

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