Bioinformatics and phylogenetic analysis for 5′ UTR region of neuropeptide Y gene and its association with body weight and egg production traits in Fars native chickens

Document Type : Research Paper

Authors

1 Former M.Sc. Student in Animal Breeding, Department of Animal Science, School of Agriculture, Shiraz University, Iran

2 Associate Professor, Department of Animal Science, School of Agriculture, Shiraz University, Iran

3 Professor, Institute of Biotechnology, Shiraz University, Iran

4 Ph.D. Candidate, Institute of Biotechnology, Shiraz University, Iran

5 Instructor, Animal Science Department, Fars Agriculture and Natural Resource Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran

Abstract

The aim of this study was bioinformatics and phylogenetic analysis for 5′UTR region of neuropeptideY (NPY) gene and its association with body weight and egg production traits in Fars native chickens. For this porpuse 200 blood samples were collected from Fars Native chickens Breeding Center (Shamsabad station). The whole genome DNA was extracted using salting out protocol. The promotor region was amplified by PCR and SNP genotyping was performed by RFLP method. The frequencies of alleles A and B were estimated as 0.46 and 0.54, respectively. The population was deviated from H-W equilibrium. Statistical analysis indicated that there is no significant association between NPY genotypes and studied traits (egg number, age at first laying, egg weight at 84 weeks and body weight at 12 weeks). The results of phylogenetic analysis showed that the sequences of the NPY gene of Fars native chickens has the most simalirity with EU447658.1andEU447659.1 sequence information. Interstingly, there is NPY gene of quail in phylogenetic analysis which can show the key role of NPY gene for regulating the physiological process.

Keywords

References

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Iranian Journal of animal Science
Volume 49, Issue 3
December 2018
Pages 453-458

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History

  • Receive Date: 29 July 2018
  • Revise Date: 10 December 2018
  • Accept Date: 12 December 2018

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