TY - JOUR ID - 69375 TI - Bioinformatics and phylogenetic analysis for 5′ UTR region of neuropeptide Y gene and its association with body weight and egg production traits in Fars native chickens JO - Iranian Journal of animal Science JA - IJAS LA - en SN - 2008-4773 AU - Jamalpour, Mehrnesa AU - Dadpasand, Mohammad AU - Atashi, Hadi AU - Niazi, Ali AU - Kharrati, Hamed AU - Hashemi, seyed Mohammadreza AD - Former M.Sc. Student in Animal Breeding, Department of Animal Science, School of Agriculture, Shiraz University, Iran AD - Associate Professor, Department of Animal Science, School of Agriculture, Shiraz University, Iran AD - Professor, Institute of Biotechnology, Shiraz University, Iran AD - Ph.D. Candidate, Institute of Biotechnology, Shiraz University, Iran AD - Instructor, Animal Science Department, Fars Agriculture and Natural Resource Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran Y1 - 2018 PY - 2018 VL - 49 IS - 3 SP - 453 EP - 458 KW - Native fowl KW - Neuropeptide Y KW - 5`UTR DO - 10.22059/ijas.2018.262962.653652 N2 - The aim of this study was bioinformatics and phylogenetic analysis for 5′UTR region of neuropeptideY (NPY) gene and its association with body weight and egg production traits in Fars native chickens. For this porpuse 200 blood samples were collected from Fars Native chickens Breeding Center (Shamsabad station). The whole genome DNA was extracted using salting out protocol. The promotor region was amplified by PCR and SNP genotyping was performed by RFLP method. The frequencies of alleles A and B were estimated as 0.46 and 0.54, respectively. The population was deviated from H-W equilibrium. Statistical analysis indicated that there is no significant association between NPY genotypes and studied traits (egg number, age at first laying, egg weight at 84 weeks and body weight at 12 weeks). The results of phylogenetic analysis showed that the sequences of the NPY gene of Fars native chickens has the most simalirity with EU447658.1andEU447659.1 sequence information. Interstingly, there is NPY gene of quail in phylogenetic analysis which can show the key role of NPY gene for regulating the physiological process. UR - https://ijas.ut.ac.ir/article_69375.html L1 - https://ijas.ut.ac.ir/article_69375_ddec6878cf2e1468659a5a6536bc0b5d.pdf ER -