Molecular analysis of antibiotic resistance genes in Escherichia coli from broiler chickens in shahrebabak by Multiplex PCR Technique

Document Type : Research Paper

Authors

1 Ph. D. Candidate, Department of Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Professor, Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

3 Professor, Department of Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

Avian pathogenic Escherichia coli (APEC) is responsible for an extremely wide range of extra intestinal diseases in poultry, including Colibacillosis and Cellulitis. There is the problem of antibiotic resistance all over the world. The aim of this study is the molecular study of antibiotic resistance genes, such as: aac, aad, qnr, tet, anr, sul and determination of antibiotic susceptibility. 83 strains of E. coli of Colibacillosis cases and 34 strains of Cellulitisin cases were gotten from in poultry slaughterhouses in Shahrebabak province within 6 months in 2017. Strains of E. coli were confirmed by biochemical methods. The results of the study showed that 63.85% for the tetA gene, 62.65% for the tetB gene were positive. The abundance of genes qnrs (49.3%), qnrB (24.09%), sul1 (28.91%), dhfrv (31.32%), dhfrA (26.50%), aac (3)-1(25.66%), aadA (22.89%), floR (28.91%), sul1 (33.13%), qnrA (12.30%). None of the specimens was positive for blaoxa, blaCTX, blasHV, blaTEM, genes. In this study, it was found that all of the suppositories were resist ant to one or more antibiotics between 40% and 90%. The highest resistance to antibiotics was tetracycline, sulfamethoxazole, chloramphenicol, terimetoprim. In this study, antibiotic resistance was found to be due to the presence of resistance genes. Recognition of resistance pattern and microorganisms susceptibility to antibiotics have an effective role on correct and suitable selection of antibiotic and infection control.

Keywords

References

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Iranian Journal of animal Science
Volume 49, Issue 2 - Serial Number 2
September 2018
Pages 203-211

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History

  • Receive Date: 10 May 2018
  • Revise Date: 14 June 2018
  • Accept Date: 16 June 2018

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