The Effect of Intra Amniotic Injection of Zinc-Methionine and Nano Zinc-Methionine on Metallothionein Gene Expression in the Broiler chickens

Document Type : Research Paper

Authors

1 Ph.D. Student, Department of Animal Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran

2 Associate Professor, Department of Animal Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran

3 Assistant Professor, Department of Animal Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran

Abstract

The effects of intra amniotic injection of zinc-methionine and nano zinc-methionine on metallothionein mRNA expression of chicken’s liver and intestine were studied. Four hundred fertiled eggs (Ross 308 strain) were allocated to 4 treatments, 4 replicates/ treatment and 25 eggs per replicate using a CRD (Complete Random Design) design. Experimental treatments were: 1- no injection (negative control), 2- injection of 1 ml 0.9% saline solution (positive control), 3- injection of 1 ml 25% zinc- methionine solution and 4- injection of 1 ml 25% nano-zinc- methionine solution. On day 17 of incubation, eggs were injected using insulin syringes. Results showed that in Ovo injection lead to no significant effect on hatchability. The injection of zinc-methionine and nano zinc-methionine caused significant (P<0.01) increase in chicks weight at hatch, and a week after that. A similar result was obtained for liver and intestine samples. Groups treated with zinc-methionine and nano zinc-methionine showed significant (P<0.01) increase in metallothionein mRNA expression in liver and intestine and remained significant (P<0.01) at day 7 after hatch. Experimental treatments significantly (P<0.01) increased the liver and intestine metallothionein content at day 1 and 7 after hatch. Conclusion is that in ovo injection of zinc-methionine and nano zinc-methionine lead to increase body weight at first 3weeks of broiler rearing.

Keywords

References

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History

  • Receive Date: 03 August 2016
  • Revise Date: 09 January 2017
  • Accept Date: 04 February 2017

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