%0 Journal Article %T The Effect of Intra Amniotic Injection of Zinc-Methionine and Nano Zinc-Methionine on Metallothionein Gene Expression in the Broiler chickens %J Iranian Journal of animal Science %I پردیس کشاورزی و منابع طبیعی دانشگاه تهران %Z 2008-4773 %A Razani, Kolsoom %A Mottaghitalab, Majid %A Heseini Moghaddam, Seyed Hosein %D 2017 %\ 02/19/2017 %V 47 %N 4 %P 621-632 %! The Effect of Intra Amniotic Injection of Zinc-Methionine and Nano Zinc-Methionine on Metallothionein Gene Expression in the Broiler chickens %K broiler %K In Ovo feeding %K metallothionein %K nano zinc methionine %R 10.22059/ijas.2017.213018.653459 %X The effects of intra amniotic injection of zinc-methionine and nano zinc-methionine on metallothionein mRNA expression of chicken’s liver and intestine were studied. Four hundred fertiled eggs (Ross 308 strain) were allocated to 4 treatments, 4 replicates/ treatment and 25 eggs per replicate using a CRD (Complete Random Design) design. Experimental treatments were: 1- no injection (negative control), 2- injection of 1 ml 0.9% saline solution (positive control), 3- injection of 1 ml 25% zinc- methionine solution and 4- injection of 1 ml 25% nano-zinc- methionine solution. On day 17 of incubation, eggs were injected using insulin syringes. Results showed that in Ovo injection lead to no significant effect on hatchability. The injection of zinc-methionine and nano zinc-methionine caused significant (P<0.01) increase in chicks weight at hatch, and a week after that. A similar result was obtained for liver and intestine samples. Groups treated with zinc-methionine and nano zinc-methionine showed significant (P<0.01) increase in metallothionein mRNA expression in liver and intestine and remained significant (P<0.01) at day 7 after hatch. Experimental treatments significantly (P<0.01) increased the liver and intestine metallothionein content at day 1 and 7 after hatch. Conclusion is that in ovo injection of zinc-methionine and nano zinc-methionine lead to increase body weight at first 3weeks of broiler rearing. %U https://ijas.ut.ac.ir/article_61167_626bc1a4d43866a4b77a2f25c3e42211.pdf