Document Type : Research Paper
Postgraduate Student, University College of Agriculture & Natural resources, University of Tehran, Karaj, Iran
Professor, University College of Agriculture & Natural resources, University of Tehran, Karaj, Iran
Assistant Professor, University College of Agriculture & Natural resources, University of Tehran, Karaj, Iran
Professional Doctorate, National Animal Breeding Center and Promotion of Animal Products, Iran
The purpose of this study was to determine the effects of different concentrations of vitamin E (Vit. E) in the quality of preserved stallion semen at 5ᵒC for a period of 48 h. Semen was collected and most (≥90%) seminal plasma removed by centrifugation at 600 × g. Pooled samples divided in four equal parts and diluted with extenders that was supplemented with different concentrations of Vit. E [(0 (control, C), 5 and 10 mM)] and DMSO positive control group [as vitamin E solvent, (DC)] to a final concentration of 50 × 106 sperm/ml. Motility, viability, plasma membrane integrity, total abnormality and lipid peroxidation were determined at 0, 6, 24 and 48h. The results showed that 5mM vitamin E resulted in higher viability and plasma membrane integrity compared to control after 48 h of storage (P<0.05). Different concentrations of vitamin E had no significant effects on sperm motility and total abnormality compared to C and DC extender (P>0.05). Also, 5mM Vit. E was decreased lipid peroxidation compared C and DC extenders after 48h of storage (P<0.05). In conclusion, between above-mentioned different concentrations, 5mM Vit. E could improve Caspian stallion sperm quality during cooled-storage at 5°C.