TY - JOUR ID - 51743 TI - The effect of different levels of Vitamin E in semen extender during the cooling on Caspian stallion sperm quality JO - Iranian Journal of animal Science JA - IJAS LA - en SN - 2008-4773 AU - Yousefian, Iman AU - Zare-shahneh, Ahmad AU - Zhandi, Mahdi AU - Bahreini, Majid AU - Izadpanah, Ghazaleh AU - Noei, Reza AU - Emamverdi, Mojtaba AD - Postgraduate Student, University College of Agriculture & Natural resources, University of Tehran, Karaj, Iran AD - Professor, University College of Agriculture & Natural resources, University of Tehran, Karaj, Iran AD - Assistant Professor, University College of Agriculture & Natural resources, University of Tehran, Karaj, Iran AD - Professional Doctorate, National Animal Breeding Center and Promotion of Animal Products, Iran Y1 - 2014 PY - 2014 VL - 45 IS - 1 SP - 1 EP - 8 KW - Caspian stallion semen KW - Cooling KW - Lipid Peroxidation KW - Sperm quality KW - vitamin E DO - 10.22059/ijas.2014.51743 N2 - The purpose of this study was to determine the effects of different concentrations of vitamin E (Vit. E) in the quality of preserved stallion semen at 5ᵒC for a period of 48 h. Semen was collected and most (≥90%) seminal plasma removed by centrifugation at 600 × g. Pooled samples divided in four equal parts and diluted with extenders that was supplemented with different concentrations of Vit. E [(0 (control, C), 5 and 10 mM)] and DMSO positive control group [as vitamin E solvent, (DC)] to a final concentration of 50 × 106 sperm/ml. Motility, viability, plasma membrane integrity, total abnormality and lipid peroxidation were determined at 0, 6, 24 and 48h. The results showed that 5mM vitamin E resulted in higher viability and plasma membrane integrity compared to control after 48 h of storage (P<0.05). Different concentrations of vitamin E had no significant effects on sperm motility and total abnormality compared to C and DC extender (P>0.05). Also, 5mM Vit. E was decreased lipid peroxidation compared C and DC extenders after 48h of storage (P<0.05). In conclusion, between above-mentioned different concentrations, 5mM Vit. E could improve Caspian stallion sperm quality during cooled-storage at 5°C. UR - https://ijas.ut.ac.ir/article_51743.html L1 - https://ijas.ut.ac.ir/article_51743_d3b3b74cd12ce2eea70af0bfad14ba6c.pdf ER -