Document Type : Research Paper


1 Former Post Graduated Student, Faculty of Agriculture, Tarbiat Modarres University, Tehran, Iran

2 Faculty Member Department of Animal Sciences, Faculty of Agriculture, Tarbiat Modarres University, Tehran, Iran


The effect of three levels (0, 28.65, 57.31mg /200mg substrate) of Golpar (Heracleum persicum: HP) on in vitro rumen ecosystem and on methane production was assessed. Gas Production (GP) was recorded from 1 to 54h past of incubation. The parameters studied were In Vitro Gas Production (IVGP), methane production, ammonia-N concentration (NH3-N), In Vitro Organic Matter Degradability (IVOMDe), Partitioning Factor (PF), microbial mass, and total Volatile Fatty Acids (VFAs) concentration. The effect of different Golpar levels on the total protozoa population, subfamilies of Entodiniinae, Ophryscolecinae, Diplodiniinae, and Isotrichdae family were also studied. The secondary Haracleum persicum (HP) metabolites significantly decreased rate of fermentation, gas production from insoluble fraction (b), and as well gas production at 24 and 54 h Past of incubation. At first and second levels of application of HP, methane gas production decreased by 17.3 and 11.6 present, respectively. The inclusion of HP plant while reducing NH3-N concentration improved PF index. The addition of HP increased microbial mass at the two levels of qpplication by 17 and 18 percent, respectively. The introduction of this medical plant decreased the concentration of VFAs and as well, the acetic acid concentration.The reduction in total protozoa population, Entodiniinae subfamily and Isotrichdae family, also, revealed an antiprotozoal effect of HP. It can finally be suggested that HP plant bears the potential to positively manipulate in vitro ruminal fermentation through NH3-N concentrationreduction microbial mass production promotion and reduction in methane gas production.