Document Type : Research Paper

Authors

1 Graduate Student University College of Agriculture & Natural Resources, University of Tehran

2 Associate Professors, University College of Agriculture & Natural Resources, University of Tehran

Abstract

Mycotoxins are secondary metabolites produced by fungi following their infestation of food crops.
One of the effective approaches to control aflatoxicosis is the use of adsorbents. The aim thus
followed in the present study was a comparison of various adsorbents viz. Bentonite, Zeolite, vs. an
organic commercial adsorbent (Mycosorb) and a mineral – organic commercial adsorbent (Biotox)
regarding their potential to adsorb aflatoxin B1. AFB1- contaminated rice was prepared by its
inoculation with the Aspergillus parasiticus, strain type PTTC 5286. levels of aflatoxin, present in the
contaminated rice were determined through HPLC. AFB١ was chloroform extracted thrice, by having
rice soaked in chloroform. Adsorbents were individually mixed at three different ratios with AFB١
(1:1000, 1:5000 and 1:15000, w/w), in a buffer solution, centrifuged and the supernatant evaluated for
Aflatoxin B١ conted using Aflatoxin B١ ELISA kit. The design employed was a completely
randomized one of: 4×3 factorial arrangement. Results indicated that at 1:15000 Aflatoxin to
adsorbent ratio, Zeolite, Mycosorb and Biotox sequestered (adsorbed) about 0.8, 0.81 and 0.83 of the
AFB١. This efficacy decreased with the amounts of adsorbents reduced. Bentonite had the poorest
sequestering efficacy, with 0.38 being the maximum value obtained for the 1:15000 Aflatoxin to
adsorbent ratio treatment. There was a significant difference observed among the three ratios and as
well between Biotox and other adsorbents for their percentages of AFB1 adsorption (P<0.001).

Keywords