The effects of partial replacing of dietary starch with saturated or essential unsaturated fatty acids on production performance, ruminal fermentation and blood parameters in lactating cows

Document Type : Research Paper

Authors

Department of animal science- College of agriculture and natural resources, University of Tehran, Karaj , Iran

Abstract

In this study, 21 Holstein lactating cows with average 105/7± 34.5 days in milk used to evaluate partial replacing of starch with saturated or unsaturated fatty acids on milk yield and composition, blood and rumen parameters and nutrient digestibility. Cows were used in a completely randomized design with three diets included: 1. Diets supplemented with calcium salts of unsaturated fatty acids (1/9% of diet DM).2. Diet supplemented with saturated fatty acids mainly palmitic acid (1/9% of diet DM). 3. Carbohydrate diet (no fat supplementation). The study period was 45 days, 10 days for adaptation and 35 days for data collection. During this 35-day milk production and feed intake were recorded daily. Milk and feed samples were taken weekly. At the end of the study period blood samples, rumen fluid and feces were collected. The results showed that milk fat percentage and feed efficiency increased use of supplements and treatments had significant effects. The blood urea nitrogen was increased in unsaturated fat supplements in compare to carbohydrate group but in saturated fat supplement was increased (p <0/05). Milk fatty acid profile showed that the amount of linoleic acid and linoleic acid significantly increased in unsaturated fat supplement group and palmitic acid significantly increased in saturated fat supplement group (p <0/05). Overall, supplementing calcium salts of unsaturated essential fatty acids can support same milk production and composition but improve milk fatty acids profile to improve the health of the consumer.

Keywords

Main Subjects


Extended Abstract

Introduction

Fat supplements have a great role in livestock diets today. Fats as energetic compounds can increase energy in diets. Also, today, special attention is paid to the consumption of fat in livestock and their products, so that scientists not only do not prohibit other people from consuming fat sources, but also strongly recommend the consumption of certain types of fat to prevent certain diseases such as cardiovascular diseases, high blood pressure, arthritis, diabetes, obesity, cancers and osteoporosis. With the increase in production capacity in dairy and fattening animals, as well as paying attention to the quantitative and qualitative aspects of the types of fat they produce, the feeding of different forms of fat has attracted the attention of animal nutrition researchers, and with the positive results, practical aspects have also been applied in breeding farms.

 

 

 

Materials and methods

 This experiment was carried out in the educational-research station of the Department of Animal Science, Faculty of Agriculture, University of Tehran. 21 Holstein lactating cows with average 105/7± 34.5 days in milk used to evaluate partial replacing of starch with saturated or unsaturated fatty acids on milk yield and composition, blood and rumen parameters and nutrient digestibility. Cows were used in a completely randomized design with three diets included: 1. Diets supplemented with calcium salts of unsaturated fatty acids (1/9% of diet DM).2. Diet supplemented with saturated fatty acids mainly palmitic acid (1/9% of diet DM). 3. Carbohydrate diet (no fat supplementation). The study period was 45 days, 10 days for adaptation and 35 days for data collection.

 

Results and discussion

The results of this experiment showed that the use of both types of fat supplements (SFA and PUFA) caused a significant increase in milk fat percentage, ammonia nitrogen concentration in the rumen liquid, and feed consumption efficiency of cows. The concentration of blood urea nitrogen was also increased as well palmitic acid in the milk of cows that were fed with the supplement of saturated palmitic fatty acids. The addition of the calcium salt supplement of unsaturated fatty acids significantly increased the digestibility of the ether extract, and also the use of this type of supplement in the feeding of lactating cows showed an increase in the amount of linoleic acid, linolenic acid and PUFA in milk fat. Therefore, this improves the fatty acid profile of cow's milk in human nutrition too.

 

Author Contributions

In present research authors contributions were Mehdi Dehghan banadaky;  Conceptualization, validation, funding acquisition, editing, supervision, methodology and project administration. Farhang Fatehi; Software and formal analysis. Bakhtiyar Babaei; review, resources, data curation, writing original and draft preparation.

All authors have read and agreed to the published version of the manuscript. All authors contributed equally to the conceptualization of the article and writing of the original and subsequent drafts.

 

Data Availability Statement

Data available on request from the corresponding author.

 

Acknowledgements

The authors would like to thank the head of faculty of agriculture and natural resources, University of Tehran also the manager of Research station, department of animal science. Author thanks Kimiya Danesh Alvand company for financial support of present research.

 

Ethical considerations

The study was approved by the Ethics Committee of the University of Tehran (Ethical code: IR.7108004/6/41). The authors avoided data fabrication, falsification, plagiarism, and misconduct.

 

Conflict of interest

The author declares no conflict of interest.

REFERENCES
Allen, M. S. (2000). Effects of diet on short-term regulation of feed intake by lactating dairy cattle. Journal of dairy science83(7), 1598-1624.
AOAC. (1990). Official methods of analysis: Aoac Washington, DC.
Bernard, J. K. (2009). Performance and metabolic measures of lactating dairy cows fed diets supplemented with either mostly saturated or more unsaturated fatty acids.
Boerman, J., Potts, S., VandeHaar, M., & Lock, A. (2015). Effects of partly replacing dietary starch with fiber and fat on milk production and energy partitioning. Journal of dairy science, 98(10), 7264-7276.
Bruns, H., Hippen, A., Kalscheur, K., & Schingoethe, D. (2015). Inclusion of various amounts of steam-flaked soybeans in lactating dairy cattle diets. Journal of dairy science, 98(10), 7218-7225.
Cediel, D., Silva, R. R., da Silva, F. F., Santos, L. V., da Silva, A. P. G., da Conceição Santos, M., . . . Silva, J. W. D. (2022). Fatty acid profile of beef from crossbred steers supplemented in grazing
Council, N. R. (2001). Nutrient requirements of dairy cattle: 2001: National Academies Press.
dos Santos Neto, J., Silva, J., Meschiatti, M., de Souza, J., Negrão, J., Lock, A., & Santos, F. (2022). Increasing levels of calcium salts of palm fatty acids affect production responses during the immediate postpartum and carryover periods in dairy cows. Journal of dairy science, 105(12), 9652-9665.
Erickson, P. S., & Kalscheur, K. F. (2020). Nutrition and feeding of dairy cattle Animal Agriculture (pp. 157-180): Elsevier.
Hu, L., Shen, Y., Zhang, H., Ma, N., Li, Y., Xu, H., ... & Li, J. (2024). Effects of dietary palmitic acid and oleic acid ratio on milk production, nutrient digestibility, blood metabolites and milk fatty acids profile of lactating dairy cows. Journal of Dairy Science.
Giannuzzi, D., Capra, E., Bisutti, V., Vanzin, A., Marsan, P. A., Cecchinato, A., & Pegolo, S. (2023). Methylome-wide analysis of milk somatic cells upon subclinical mastitis in dairy cattle. Journal of dairy science.
González, L., Moreno, T., Bispo, E., Dugan, M. E., & Franco, D. (2014). Effect of supplementing different oils: Linseed, sunflower and soybean, on animal performance, carcass characteristics, meat quality and fatty acid profile of veal from “Rubia Gallega” calves. Meat Science, 96(2), 829-836.
Gordiano, L., Ferreira, F., Ribeiro, C., de Carvalho, G., Silva, F., de Araújo, M., . . . de Freitas Jr, J. (2023). Association between chitosan and unsaturated fatty acids supplementation on ruminal fermentation, digestive metabolism, and ruminal kinetics in beef heifers. Livestock Science, 271, 105216.
Harvatine, K., & Allen, M. (2005). The effect of production level on feed intake, milk yield, and endocrine responses to two fatty acid supplements in lactating cows. Journal of dairy science, 88(11), 4018-4027.
Ichihara, K. I., & Fukubayashi, Y. (2010). Preparation of fatty acid methyl esters for gas-liquid chromatography [S]. Journal of lipid research51(3), 635-640.‏
Khalilvandi-Behroozyar, H., Mohtashami, B., Dehghan-Banadaky, M., Kazemi-Bonchenari, M., & Ghaffari, M. H. (2023). Effects of fat source in calf starter on growth performance, blood fatty acid profiles, and inflammatory markers during cold season. Scientific Reports, 13(1), 18627.‏
Khatkar, S. K., Khatkar, A. B., Mehta, N., Kaur, G., Dhull, S. B., & Prakash, S. (2023). Effective strategies for elevating the techno-functional properties of milk protein concentrate. Trends in Food Science & Technology, 104169.
Khorshidi, K. J., Karimnia, A., Gharaveisi, S., & Kioumarsi, H. (2008). The effect of monensin and supplemental fat on growth performance, blood metabolites and commercial productivity of Zel lamb. Pakistan Journal of Biological Sciences: PJBS, 11(20), 2395-2400.‏
Lidauer, M., Negussie, E., Mäntysaari, E., Mäntysaari, P., Kajava, S., Kokkonen, T., . . . Mehtiö, T. (2023). Estimating breeding values for feed efficiency in dairy cattle by regression on expected feed intake. animal, 17(9), 100917.
Lock, A. L., Bauman, D. E., & Jenkins, T. C. (2008, January). Understanding the biology of milk fat depression: from basic concepts to practical application. In Proc. Intermountain Nutr. Conf. Salt Lake City, UT (pp. 27-44).‏
Loosli, J. K., Maynard, L., & Lucas, H. (1944). Further Studies of the Influence of Different Levels of Fat Intake Upon Milk Secretion IV: Cornell Univ., Agricultural Experiment Station.
Lunsin, R., Pilajun, R., Cherdthong, A., & Wanapat, M. (2021). Effects of high-quality oil palm frond pellets on nutrient digestion, rumen fermentation, and production performance of lactating dairy cows. Applied Animal Science, 37(5), 574-582.
National Research Council. (2001). Nutrient requirements of dairy cattle: 2001. National Academies Press.‏
Pereira, G. M., Heins, B. J., Visser, B., & Hansen, L. B. (2022). Comparison of 3-breed rotational crossbreds of Montbéliarde, Viking Red, and Holstein with Holstein cows fed 2 alternative diets for dry matter intake, production, and residual feed intake. Journal of Dairy Science, 105(11), 8989-9000.‏
Prom, C., dos Santos Neto, J., Newbold, J., & Lock, A. (2021). Abomasal infusion of oleic acid increases fatty acid digestibility and plasma insulin of lactating dairy cows. Journal of dairy science, 104(12), 12616-12627.
Rafiee-Yarandi, H., Ghorbani, G. R., Alikhani, M., Sadeghi-Sefidmazgi, A., & Drackley, J. K. (2016). A comparison of the effect of soybeans roasted at different temperatures versus calcium salts of fatty acids on performance and milk fatty acid composition of mid-lactation Holstein cows. Journal of dairy science, 99(7), 5422-5435.
Reis, M., Cooke, R. F., Ranches, J., & Vasconcelos, J. L. M. (2012). Effects of calcium salts of polyunsaturated fatty acids on productive and reproductive parameters of lactating Holstein cows. Journal of dairy science, 95(12), 7039-7050.
Van Keulen, J., & Young, B. (1977). Evaluation of acid-insoluble ash as a natural marker in ruminant digestibility studies. Journal of Animal Science, 44(2), 282-287.
Van Soest, P. v., Robertson, J. B., & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of dairy science, 74(10), 3583-3597.
Vyas, D., Teter, B. B., & Erdman, R. A. (2012). Milk fat responses to dietary supplementation of short-and medium-chain fatty acids in lactating dairy cows. Journal of dairy science95(9), 5194-5202.‏
Waldo, D., & Jorgensen, N. (1981). Forages for high animal production: nutritional factors and effects of conservation. Journal of dairy science, 64(6), 1207-1229.
Walker, R. E., Harvatine, K. J., Ross, A. C., Wagner, E. A., Riddle, S. W., Gernand, A. D., & Nommsen-Rivers, L. A. (2022). Fatty acid transfer from blood to milk is disrupted in mothers with low milk production, obesity, and inflammation. The Journal of nutrition, 152(12), 2716-2726.
 Wang, Y., Jacome-Sosa, M. M., & Proctor, S. D. (2012). The role of ruminant trans fat as a potential nutraceutical in the prevention of cardiovascular disease. Food Research International46(2), 460-468.‏