The effect of L-carnitine on sperm parameters, testicular tissue and sperm DNA damage index in Ross strain old roosters

Articles in Press

Document Type : Research Paper

Authors

1 Jahad-e-keshavarzi- Khorramabad- Iran

2 Animal science department – School of agriculture, Lorestan university, Khoramabad,, Iran.

3 Animal science department. School of agriculture - Lorestan university- Lorestan province -Khoramabad

Abstract

L-carnitine has antioxidant properties and protect the sperm membrane from reactive oxygen substance (ROS). This component reduces the availability of lipids from oxidation. To evaluate the administration of L-carnitine on semen and blood parameters and testicular tissue in aged Ross strain roosters. Forty aged roosters with same weight and age were distributed in four treatments with 10 repetitions in standard cages. The roosters were divided into experimental treatments including control, 100, 250 and 500 mg of L-carnitine per kg of diet. The duration of the experiment was 10 weeks. The length. For habituation to sperm collection used back-abdominal rubbing method. Semen samples were transferred to the laboratory at temperature of 37°C and after initial evaluation, parameters of motility, viability, integrity of sperm plasma membrane and DNA health were evaluated. The serum samples were collected to determine parameters and testicular tissue for histological studies. The rate of motility, viability, membrane integrity and DNA integrity increased in treatments fed with L-carnitine. The maximum integrity was observed in the treatment of 500 mg/kg. The amount of cholesterol and HDL in treatment 4 (500mg) was minimum and maximum respectively, while liver enzyme alanine aminotransferase showed no significant difference in all treatments. The thickness of the germinal layer in seminiferous tubules increased significantly with the addition of 500 and 250 mg of L-carnitine. L-carnitine is able to reduce the effects of oxidative stress in aged roosters. This result can be suggested and used for important breeds that are kept for unusual period.

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Iranian Journal of animal Science

Articles in Press, Accepted Manuscript
Available Online from 10 July 2024

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History

  • Receive Date: 12 November 2023
  • Revise Date: 08 February 2024
  • Accept Date: 20 June 2024

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