The effect of using different forms of chromium on hematological parameters and antioxidant status of Afshar ewes in the transition period and their lambs under the influence of heat stress

Document Type : Research Paper

Authors

1 Department of Animal and Poultry Nutrition, Animal Science Faculty, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran.

2 Department of Animal and Poultry nutrition, Animal Science Faculty, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran.

Abstract

The present study aims to investigate the effect of different forms of chromium (inorganic chromium, chromium-methionine and chromium nanoparticles) on blood parameters, antioxidant status and immunoglobin of Afshar ewes during the transition period and lambs. They were done under the influence of heat stress. 40 pregnant Afshar ewes from 42 ± 2 days before the expected parturition in a completely randomized design. Four experimental treatments were assigned with ten replications. Experimental treatments include basic diet without chromium supplement (control), basic diet containing 3 mg of chromium in mineral form per kg of dry matter, basic diet containing 3 mg of chromium in the form of chromium-methionine per kg of dry matter and The basic diet contained 3 mg of chromium in the form of chromium nanoparticles per kilogram of dry matter. The results showed that the addition of different forms of chromium to the ewes' diet caused a significant increase in the concentration of red blood cells, hemoglobin and blood hematocrit compared to the control group (P<0.05). Adding chromium to the diet of ewes caused a significant decrease in white blood cells (P<0.05). In experimental treatments, no significant difference was observed in terms of platelets, average volume of red blood cells, average concentration of red blood cells, neutrophils, lymphocytes, monocytes, and eosinophils. The concentration of superoxide desmutase, glutathione peroxidase, catalase, triiodothyronine and tetraiodothyronine in the blood in the treatments receiving different forms of chromium also increased compared to the control group (P<0.05). The results showed that the concentration of malondialdehyde and total antioxidant capacity in ewes and lambs receiving chromium supplementation was lower than the control treatment (P<0.05). However, there was no significant difference between different experimental treatments in terms of ceruloplasmin concentration and the ratio of T4 to T3 (P<0.05). The concentration of IgG and IgM in lambs receiving different forms of chromium also increased compared to the control group (P<0.05). In general, the use of chromium, especially in the form of chromium-methionine and chromium nanoparticles, is recommended during the transition period of ewes under the influence of heat stress.

Keywords

Main Subjects

Extended Abstract

Introduction

It is very important to choose the appropriate form of chromium in the ration of ewes in the transition period and under the influence of heat stress in order to improve the performance of ewes and newborn lambs and reduce the negative effects of stress in these conditions. The present study is to investigate the effect of using different forms of chromium (inorganic chromium, chromium-methionine and chromium nanoparticles) on blood parameters, antioxidant status and immunoglobulin of Afshar ewes during the transition period and their lambs under the influence of heat stress. Done.

 

Materials and Methods

     In the summer of 1401, 40 pregnant Afshari ewes were assigned to four experimental treatments with ten replications in a completely random design from 42 ± 2 days before the expected birth. The experimental treatments include the basic diet without chromium supplementation (control), the basic diet containing 3 mg of chromium in mineral form per kg of dry matter, the basic diet containing 3 mg of chromium in the form of chromium-methionine per kg of dry matter, and the basic diet containing 3 milligrams of chromium in the form of chromium nanoparticles per kilogram of dry matter. The basic ration was adjusted according to the tables of the National Sheep Research Association and supplements were added to the basic ration daily. The ewes were all balanced for age, number of embryos, weight, expected calving date and color. The accuracy of pregnancy and the possible time of delivery were confirmed through pregnancy synchronization and ultrasound of the ewes before the experiment. The experiment started 42 days before calving and lasted for 84 days.

 

Results

    The results showed that the addition of different forms of chromium to the diet of ewes caused a significant increase in the concentration of red blood cells, hemoglobin and blood hematocrit compared to the control group (P<0.05). Adding different forms of chromium to the diet of ewes caused a significant decrease in white blood cells (P<0.05). In experimental treatments, no significant difference was observed in platelet, mean corposcular volume, mean corpuscular hemoglobin concentration, neutrophil, lymphocyte, monocyte and eosinophil blood. The concentration of superoxide desmutase, glutathione peroxidase, catalase, triiodothyronine and tetraiodothyronine in the blood in the treatments receiving different forms of chromium also increased compared to the control group (P<0.05). The results showed that the concentration of malondialdehyde and total antioxidant capacity in ewes and lambs receiving chromium supplementation was lower than the control treatment (P<0.05). However, there was no significant difference between different experimental treatments in terms of ceruloplasmin concentration and the ratio of T4 to T3 (P<0.05). The concentration of IgG and IgM in lambs receiving different forms of chromium also increased compared to the control group (P<0.05).

 

CONCLUSIONS

    In general, it is recommended to receive chromium, especially in the form of chromium-methionine and chromium nanoparticles during the period of transfer of ewes under the influence of heat stress.

References

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Iranian Journal of animal Science
Volume 55, Issue 3
September 2024
Pages 547-563

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  • Receive Date: 09 September 2023
  • Revise Date: 29 October 2023
  • Accept Date: 06 February 2024

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