Molecular analysis of pyrethroid resistance in the larvae of house fly (Musca Domestica) collected from Poultry farms in northwestern Iran

Document Type : Research Paper

Authors

1 PhD student, Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, urmia.Iran

3 Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

4 Postdoctoral student, Department of Health and Food, Faculty of Veterinary Medicine, Urmia University,urmia. Iran

Abstract

The present study was aimed to assess the house fly susceptibility to pyrethroid insecticides using molecular analysis. Flies were collected from poultry farms in four different cities of West Azerbaijan and were transferred to the laboratory to evaluate their resistance to pyrethroids using insecticide resistance bioassay. Genomic DNA was extracted from susceptible and resistant flies. Using a technique called polymerase chain reaction (PCR) amplification of specific allele (PASA), PCR was employed to reveal the presence of allelegenetic variations in the para-type sodium channel (para) gene to recognize knockdown resistance (kdr) mutation from the homozygous type of complete susceptibility (sus/sus) to the mutated-homozygous type of complete resistance (kdr/kdr) or to the mutated-heterozygous type (kdr/sus). In this study, these genotypes were targeted using specific primers to identify these genetic variations. The results of bioassay showed more than 50% resistance in all studied populations , and the highest level of resistance was reported from houseflies in Urmia city (86%). A polymerase chain reaction test for identifying kdr homo- or heterozygote house flies was used to determine the frequency of kdr. The L1014 F allele was found in all populations tested. The frequency of KDR in the studied populations was high and varied from 37% to 81%.The maximum percentage (71%) of homozygous genotype (kdr/kdr) occurred at Urmia, followed by Takab (61%), and the minimum percentage occurred at Sardasht (30%). Flies with kdr/kdr genotype were identified in all tested populations

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Iranian Journal of animal Science

Articles in Press, Accepted Manuscript
Available Online from 07 April 2024

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History

  • Receive Date: 07 August 2023
  • Revise Date: 16 January 2024
  • Accept Date: 07 February 2024

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