Effect of injection of zinc oxide nano particles on the immune response, oxidative enzyme activity of plasma and gene expression in broilers spleen tissue

Document Type : Research Paper


Department of Animal science, Faculty of animal and fisheries sciences, Sari agricultural and natural resources university, Sari, Iran


This experiment was conducted to investigate the effect of zinc oxide nanoparticls injection on immune response and performance in broilers. One hundred eight Ross308 strain chicks were injected in a completely randomized design with three treatments in four replicates and nine chicks in each replicate on the day of hatching. The treatments include: 1. Control, 2. Injection of 20 microliters of zinc oxide nanoparticls (0.5 mg of zinc oxide nanoparticls in 50 ml of saline), 3. Injection of 20 microliters of zinc oxide nanoparticles (0.6 mg of zinc oxide nanoparticles in 50 ml of saline). The zinc oxide nanoparticls solution was injected into the chickens from the neck area. The ration used for all treatments was the standard ration of Ross 308 strain. Influenza antibody titer 14 days and CBH test, 12 and 24 hours after vaccination and glutathione peroxidase activity level increased significantly in treatment 3 compared to other treatments (P<0.05). Interleukin 12 gene expression in treatment 2 and 3 was significantly higher than the control treatment (P<0.05). The relative weight of gravel in treatment 3 compared to the control treatment and the feed consumed in treatments 2 and 3 were significantly lower than the control treatment (P<0.05). The results showed that the injection of zinc oxide nanoparticls increased the general and cellular immune response, the activity of antioxidant enzymes and interleukin 12 gene expression.


Main Subjects

Extended Abstract


After using antibiotics as growth promoters in poultry feed, try to replace them with suitable options with positive properties and no negative side effects and options of natural origin such as medicinal plants, natural probiotics and organic acids. Addition of zinc in the diet of broiler chickens increases daily body weight, daily food intake and immune response and performance of broiler chickens and increases growth rate. The volume and size of nanoparticles is smaller than larger particles and due to more contact surface and higher absorption, it provides better performance and reduces the consumption of nutrients. These substances have higher cellular absorption and after entering, they spread quickly in most organs and tissues.



Oxide-nanoparticles can improve the safety and efficiency of production in broiler chickens through absorption and transport through the bloodstream instead of the small intestine.


Materials and methods

108 chicks of Ross308 strain were treated with zinc nano oxide solution in a completely randomized design with three treatments in four replicates and nine chicks in each replicate on the hatching day and were reared for 42 days. The treatments include: 1) control (without injection), 2) injection of 10 mg/mliter of zinc nano oxide solution, 3) injection of 12 mg/mliter of zinc nano oxide solution.The zinc nano oxide solution was injected into the chickens from the neck area. The ration used for all treatments was the standard ration of Ross308 strain.



CBHtest, 12hours after Phyto Hem Aglutinin injection and glutathione peroxidase activity level increased in treatment 3 compared to other treatments(P<0.05). interleukin 12 gene expression in treatments 2 and 3 was higher than the control treatment (P<0.05). The relative weight of gizzard in treatment 3 compared to the control treatment and the feed consumption in treatments 2 and 3 was less than the control treatment(P<0.05). The results showed that zinc nano oxide injection increased blood and cellular immune response, antioxidant enzyme activity and interleukin 12 gene expression.



The better bioavailability of zinc in the form of nanoparticles in the body increases the activity of enzymes related to digestion and absorption and improves the morphological characteristics of the intestine and increases the absorbed surface and the bioavailability of other nutrients, which can lead to a reduction in food consumption, which can be used as an alternative to antibiotics. By increasing and improving IL12 gene expression and affecting the proliferation of basophils, increasing the level of glutathione peroxidase, increases the immune response in broilers and improves bird health. By injecting broilers with zinc nano oxide at the right concentration and time, it is possible to deal with the production of free radicals and lipid peroxidation by increasing the level of blood antioxidant enzymes in the presence of tension and oxidative stress.

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