‏ In-vitro effects of Aquastart on theronts of Ichthyophthirius multifiliis in rainbow trout (Oncorhynchus mykiss)

Document Type : Research Paper

Authors

1 Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Academic staff/Urmia faculty of Veterinary Medicine

3 Artemia Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization, Urmia, Iran

Abstract

Rainbow trout (Oncorhynchus mykiss) is one of the economically and nutritionally important famed species of cold-water fish in Iran. Ichthyophthirius multifiliis is a parasitic infection; that causes economic losses in aquaculture industry. The present study aimed to investigate the in-vitro effect of Aquastart on I. multifiliis theronts isolated from Rainbow trout. From a rainbow trout fish farm, 20 infected fish (average weight: 20±4 gr and average length: 14±2 cm) with I. multifiliis were trapped. They were exposed to 80 non-infected fingerling fish in three phases. The theronts were isolated from throphonts on the skin and gills of infected fish and counted. The concentrations of 5, 10, 20, 50, 100, and 200 mg/kg of Aquastart were provided from stock Aquastart (400 mg/kg), and 300 theronts were added. The lethal effect of Aquastart against the examined theronts were determined along with two control groups of Malachite green (0.05 mg/kg), and 50 ml dechlorinate water without Aquastart. The minimum and maximum lethal effects of Aquastart were at 0.67 ± 0.038 (200 mg/L) and 150 ± 1.2 (5 mg/L), respectively. The duration of the lethal effect of Aquastart had a significant association with concentration. From the results of this study, it was concluded that Aquastart had a toxic effect on theronts of I. multifiliis isolated from Rainbow trout and different concentrations of Aquastart at different time durations eliminated them.

Keywords

Main Subjects

Iranian Journal of animal Science

Articles in Press, Accepted Manuscript
Available Online from 08 July 2023

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History

  • Receive Date: 04 March 2023
  • Revise Date: 21 June 2023
  • Accept Date: 21 June 2023

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