Expression of cytokine genes in spleen tissue of broiler chickens fed with different levels of oak acorn

Document Type : Research Paper

Authors

1 Associate professor of Animal Science Department, Faculty of Agriculture Science, University of Yasouj, Yasouj, Iran

2 MSc graduated of Genetics and Animal Breeding, Department of Animal Science, Faculty of Agriculture, University of Yasouj, Yasouj, Iran

3 MSc student of Genetics and Animal Breeding, Department of Animal Science, Faculty of Agriculture, University of Yasouj, Yasouj, Iran

Abstract

The aim of this study was to investigate the expression of cytokine genes in the splenic organs of broilers fed a diet containing different levels of oak acorn. For this purpose, three diets containing 0, 15% and 20% oak acorn were used to feed broiler chickens in a period of 42 days. The results showed that the expression of IFN-γ gene in the spleen organ of broiler chickens fed with 15% oak acorn showed a significant increase in respect to the control group at both 21 and 42 days of age (P<0.05). In the 20% oak acorn group, although the IFN-γ gene expression was higher than the control treatment at both ages, this difference was not significant. At the age of 21 days, the mRNA levels of IL-4 gene in the diet containing 15% oak acorn also showed a significant increase compared to the control group (p<0.01). On the other hand, at the age of 42 days the expression levels of interleukin 2 and 4 genes showed a significant decrease in the treatment of 15% oak acorn in compared to the control group (p<0.05). Furthermore, the mRNA levels of IL-5, IL-10 and IL-13 genes in treatments containing oak acorn at both the ages of 21 and 42 days showed no significant difference compared to the control group.

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Iranian Journal of animal Science

Articles in Press, Accepted Manuscript
Available Online from 08 July 2023

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History

  • Receive Date: 12 September 2022
  • Revise Date: 07 June 2023
  • Accept Date: 10 June 2023

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