Evaluation of ruminal disappearance and solubility of calcium, ‎phosphorus, sodium, magnesium and potassium from some feedstuffs ‎and calcium supplements ‎

Document Type : Research Paper


1 Department of Animal & Poultry Nutrition, Faculty of Animal Sciences Gorgan University of Agricultural ‎Sciences & Natural Resources, Gorgan, Iran

2 Department of Soil Sciences, Faculty of Water and Soil Engineering, Gorgan University of Agricultural ‎Sciences & Natural Resources, Gorgan, Iran

3 Department of Animal & Poultry Nutrition, Faculty of Animal Sciences, Gorgan University of Agricultural ‎Sciences & Natural Resources, Gorgan, Iran


The aim of this study was to investigate the ruminal disappearance (releases or solubility) of mineral elements (calcium, phosphorus, magnesium, sodium and potassium) from feedstuffs (wheat straw, sugar beet pulp, alfalfa, silage and wheat bran) and to compare the disappearance of five types of mineral supplements (mono calcium phosphate, di calcium phosphate, calcium carbonate, oyster shell and limestone). The analysis of the feed section was performed by a completely randomized design five feed and the mineral supplements section was performed. Examination of ruminal release of minerals from feeds showed that except calcium, a large part of minerals in food was released at zero time (P<0.05). Disappearance rate increased with increasing incubation time. Release order of elements in the incubation times was in the form of potassium, phosphorus, calcium, sodium and magnesium and the highest disappearance rate was for potassium and the lowest release rate was for magnesium (P<0.05). In the study of the highest release rate was obtained during 72 hours of incubation for potassium in alfalfa hay (P<0.05). In the other part of the experiment, the highest disappearance was obtained for monocalcium phosphate and the lowest for calcium carbonate in total incubation times (P<0.05). Also, monocalcium phosphate mineral supplements had the highest and calcium carbonate treatment had the lowest disappearance parameters soluble part of dry matter, and significant difference between experimental treatments for effective dry matter degradability at flow rates of 2, 5 and 8 (P<0.05). In general, the amount of potassium disappearance was the highest in all nutrients and in all incubation times, and also monocalcium phosphate had the highest rate of degradability parameters.


Aghajanzadeh Golshani, A. Maheri Sis, N. Baradaran Hasanzadeh, A. Asadi Dizaji, A. Mirzaei Aghsaghali, A. & Dolgari Sharaf, J. (2012). Determining nutrients degradation kinetics of chickpea (Cicer arietinum) straw using nylon bag technique in sheep. Open Veterinary Journal, 2, 54-57.
AOAC. (2012). Official Methods of Analysis, 19th edn. Arlington, VA, USA: Association of Official Analytical Chemists.
Bamikole, M. A. & Babayemi, O. J. (2009). Macro-mineral utilization in West African dwarf goats fed combinations of fertilized and unfertilized Guinea grass (Panicum maximum, cv. ntchisi), Verano stylo (Stylosanthes hamata cv Verano) and a concentrate mix. Livestock Research for Rural Development, 21 (9), 1-9.
Bravo, D. & Mesch, y. F. (2003). Versune révision des recommandations d´apports en phosphore chez le ruminant. Animal Production Science, 16, 19-26.
Bravo, D., Meclly, F., Bogaert, C. & Sauvant, D. (2000). Ruminal phosphorus availability from several feedstuffs measured by the nylon bag technique. Reproduction Nutrition, 40, 149-162.
Čerešňáková, Z., Fľak, P., Chrenková, M., Poláčiková, M. & Gralak, M. A. (2008). Ruminal phosphorus release from selected forages determined by the in Sacco technique. Slovak Journal of Animal Science, 41 (3), 146-150.
Ceresnakova, Z., Flak, P., Polachikova, M. & Chrenkova, M. (2007). In Sacco macrominral release from selected forages. Czech Journal of Animal Science, 52, 175-182.
Ceresnakova, Z., Fľak, P., Poláčiková, M. & Chrenková, M. (2005). In Sacco NDF degradability and minerals release from the selected forages in the rumen. Czech Journal of Animal Science, 50, 320-328.
Ceresnakova, Z., Žitňan, R., Sommer, A., Kokardová, M., Szakács, J., Ševčík, A. & Chrenková, M. (2000). Parameters of degradability of pasture herbage cell walls and organic matter. Czech Journal of Animal Science, 45, 139-144.
Correac, H. J. (2006). Kinetics of macro-mineral release in the rumen from Kikuyu grass (Pennisetum clandestinum) harvested at two cutting intervals. Livestock Research for Rural Development, 18, 1-14.
Costa, R. M., Ponsano, E. H. G., Souza, V. C. & Malafaia, P. (2016). Reduction of phosphorus concentration in mineral supplement on fertility rate, maternal ability and costs of beef cows reared in pastures of Urochloa decumbens. Tropical Animal Health and Production, 48, 417-422.
Esser, N. M., Hoffman, P. C., Coblentz, W. K., Orth, M. W. & Weigel, K. A. (2009). The effect of dietary phosphorus on bone development in dairy heifers. Journal of Dairy Science, 92, 1741-1749.
Evitayani, M., Fariani, A., Warly, L., Itani, T. & Fujihara, T. (2011). Dry matter and macro mineral dissapereance of selected grass in West Sumatra, Indonesia. Journal of Stored Products and Postharvest Research, 2, 104-109.
Field, A. C. (1981). Some thoughts on dietary requirements of macro-elements for ruminants. Proceedings of the Nutrition Society, 40, 267-272.
Flachowsky, G., Grun, M., Polzin, S. & Kronemann, H. (1994). In sacco dry matter degradability and Ca, Mg, and P disappearance from Italian ryegrass, alfalfa hay and wheat straw in sheep and goats. Journal of Animal Physiology and Animal, 71, 57-64.
Galdamez, N. W., Coffey, K. P., Coblentz, W. K., Scarbrough, D. A., Turner, J. E., Kegley, E. B., Johnson, Z. E., Kellogg, D. W., Gunsaulis, J. L. & Daniels, M. B. (2003). In situ solubility of selected macrominerals from common bermudagrass fertilized with different nitrogen rates and harvested on two dates. Animal Feed Science and Technology, 111, 203-221.
Ghasemi Hodk, M., Ghoorchi, T., Zerehdaran, S., Tatar, A. & Ghorbani, B. (2015).The effects of different dietary levels and particle size of Glauconite and aluminosilicates on dry matter ruminal disappearance and digestibility, feed intake, fecal moisture and blood parameters in Dalagh sheep. Animal Production Research, 4 (2), 21-37. (In Farsi)
Goff, J. P. (2006). Major advances in our understanding of nutritional influences on bovine health. Journal of Dairy Science, 89, 1292-1301.
Gralak, M. A., Sciezyynska, A., von Keyserlingk, M. A. G. & Shelford, J. (1997). In situ rumen release of some minerals in dairy cows. Arbeitstagung Mengen und Sputenelemente, Dezember, Jena, BRD, 771-777.
Hu, W., Murphy, M. R., Constable, P. & Block, E. (2007). Dietary cation- anion difference effects on performance and acid-base status of dairy cows postpartum. Journal of Dairy Science, 90, 3367-3375.
Ibrahim, M. N. M., Zemmelink, G. & Tamminga, S. (1998). Release of mineral elements from tropical feeds during degradation in the rumen. Asian-Australasian Journal of Animal Sciences, 11, 530-537.
Islam, M. R., Nishida, T., Ando, S., Ishida, M., Yoshida, N. & Arakawa, M. (2001). Effect of variety, stage of maturity and botanical fractions on phosphorous concentration and in situ degradability characteristics of phosphorus of whole crop rice silage. Animal Feed Science and Technology, 93, 237-248.
Khorasani, G. R. & Armstrong, D. G. (1990). Effect of sodium and potassium level on the absorption of magnesium and other macro-minerals in sheep. Livestock Production Science, 24, 223-235.
Ledoux, D. R. & Martz, F. A. (1991). Ruminal solubilization of selected macro minerals from forage and diets. Journal of Dairy Science, 74, 1654-1661.
Lieu, P. T., Heiskala, M., Peterson, P. A. & Yang, Y. (2001). The roles of iron in health and disease. Molecular Aspects of Medicine, 22, 1-87.
Martinez, N., Risco, C. A., Lima, F. S., Bisinotto, R. S., Greco, L. F., Ribeiro, E. S., Maunsell, F., Galvao, K. N. & Santos, J. E. P. (2012). Evaluation of peripartal calcium status, energy profile, and neutrophil function in dairy cows at low or high risk of developing uterine diseases. Journal of Dairy Science, 95, 7158-7172.
McDowell, L. R., Williams, S. N., Hidiroglou, N., Njeru, C. A., Hill, G. M., Ochoa, L. & Wilkinson, N. S. (1996). Vitamin E supplementation for the ruminants. Animal Feed Science Technology, 60, 273-296.
Mjoun, K., Kalscheur, K. F., Hippen, A. R. & Schingoethe, D. J. (2000). Ruminal phosphorus disappearance from corn and soybean feedstuffs. Journal of Dairy Science, 91, 3938-3946.
NRC. (1985). Nutrient Requirements of Sheep. 6th Edition, National Academy of Sciences, National Research Council, Washington, D.C.
Ørskov, E. R. & McDonald, I. (1979). The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of Agricultural Science, 92, 499-503.
Paik, I. K. (2001). Application of chelated minerals in animal production. Asian-Australasian Journal of Animal Sciences, 14, 191-8.
Pal, D. T., Singh, P., prasad, C. S. & Sampath, K. T. (2011). Minerals release kinetics in the rumen from five commonly available fodders. The Indian Journal of Animal Sciences, 18, 1-2.
Playne, M.J., Echcvarria, M.G. & Megarrity, R.G. (1978). Release of nitrogen, sulphur, phosphours, calcium, magnesium, potassium and sodium from four tropicalhays during their digestion in nylon bags in the rumen. Journal of the Science of Food and Agriculture, 29, 520-528.
Prados, L. F., Valadars Filho, S. C., Santos, S. A., Zanetti, D., Nunes, A. N., Costa, D. R., Mariz, L. D. S., Detmann, E., Amaral, P. M., Rodrigues, F.C. & Valadares, R. F. D. (2016). Reducing calcium and phosphorus in crossbred beef cattle diets: impacts on productive performance during the growing and finishing phase. Animal Production Science, 56, 1643-1649.
Rooke, J. A., Akinsoyinu, A. O. & Armstrong, D. G. (1983). The release of mineral elements from grass silages incubated in Sacco in the rumens of Jersey cattle. Grass and Forage Science, 38, 311-316.
Saiga, S. & Oikawa. (1995). Releasa of macrominerals from cross section of threeforage grass during water–soaking. Japanese Journal of Grassland Science, 40, 381-389.
SAS. (2004). Software Version 9.1: Statistics. SAS Institute, Cary, NC.
Serra, S. D., Serra, A. B., Ichinohe, T. & Fujihara, T. (1995). Ruminal solubilization of macrominerals in selected Philippine forages. Asian-Australasian Journal of Animal Sciences, 9, 75-81.
Suttle, N. F. (2010). Mineral Nutrition of Livestock, 4th ed. CAB International. 368 Oxford. UK.
Zanetti, D., Godoi, L. A., Estrada, M. M., Engle, T. E., Silva, B. C., Alhadas, H. M., Chizzotti, M. L., Prados, L. F., Renno, L.N. & Valadares Filho, S. C. (2017). Estimating mineral requirements of Nellore beef bulls fed with or without inorganic mineral supplementation and the influence on mineral balance. Journal of Animal Science, 95, 1696-1706.