Effects of plant extracts of Camellia sinensis and Thymus vulgaris on ‎sporulation of Eimeria oocysts of broiler exereta under laboratory ‎conditions

Document Type : Research Paper


Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran‎


Coccidiosis is a parasitic protozoal disease throughout the world caused by Eimeria species. This disease is also common in Iran. There are many anticoccidial drugs, however; new drugs release are essential due to drug resistance. Green tea (Camellia sinensis) is a plant with antioxidant against diseases and thyme (Thymus vulgaris) has also antibacterial, antioxidant and antifungal effects. The anticoccidial drugs prescription are accompany with drug resistance and their poultry meat residue. Thus, the present study was aimed to investigate the effect of C. sinensis and T. vulgaris extracts on sporulation of Eimeria species oocysts of broiler chicken exereta. For this purpose, fecal samples were collected from broiler chicken farms of West Azerbaijan province, Iran. Oocysts were collected using floatation technique. A total number of 5×104 oocysts per mL exposed with different concentrations (20, 40, 60, 80 mg/ml) of C. sinensis and T. vulgaris in different times. Results indicated. C. sinensis and T. vulgaris extracts anticoccidial effect on sporulation of Eimeria oocysts was dose dependent. C. sinensis and T. vulgaris extracts inhibit sporulation of Eimeria oocysts at concentration of 80 and 60 mg/mL after 72h and 48h, respectively. It was concluded that C. sinensis and T. vulgaris extracts can inhibit sporulation of Eimeria oocysts under laboratory condition.


Abbas, R. Z., Iqbal, Z., Khan, M. N., Zafar, M. A., & Zia, M. A. (2010). Anticoccidial activity of Curcuma longa L. in broilers. Brazilian Archives of Biology and Technology, 53, 63-67.
Abbas, R. Z., Iqbal, Z., Blake, D., Khan, M. N., & Saleemi, M. K. (2011). Anticoccidial drug resistance in fowl coccidia: the state of play revisited. World's Poultry Science Journal, 67(2), 337-350.
Abbas, R. Z., Colwell, D. D., & Gilleard, J. (2012). Botanicals: an alternative approach for the control of avian coccidiosis. World's Poultry Science Journal68(2), 203-215.
Arjmand Shabestary, A., Khaloei, M., Arjomandzadegan, M., Eslamirad, Z., & Ghasemikhah, R. (2017). Effects of Zataria, Mentha pulegium, Oregano spp Essential Oil and hydroalcholic extract of Hypericum perforatum on cyst of Acanthamoeba spp. in vitro. Journal of Arak University Medical Sciences, 20(8), 1-8. (In Persian)
Azadbakht, M., Ziaei, H., Abdollahi F,Shabankhani, B. (2003). Effect of Essential Oil of Artemisia artemisia, Shirazi Thyme and Case on Trichomonas vaginalis. Medicinal Plants, 8, 35-40.
Barati M, Sharif A, Sharififar F. Antiallomagnetic (2009). Effects of extracts of Zataria multiflora, Peganum and Myrtus were extracted by colorimetric method. Journal of Kerman University of Medical Sciences, 7(1), 32-36. (In Persian)
Bozkurt, M., Giannenas, I., Küçükyilmaz, K., Christaki, E., & Florou-Paneri, P. (2013). An update on approaches to controlling coccidia in poultry using botanical extracts. British Poultry Science54(6), 713-727.
Chan, E. W., Soh, E. Y., Tie, P. P., & Law, Y. P. (2011). Antioxidant and antibacterial properties of green, black, and herbal teas of Camellia sinensisPharmacognosy Research3(4), 266.
Chatterjee, S., Ghosh, R., & Mandal, N. C. (2020). Inhibition of biofilm-and hyphal-development, two virulent features of Candida albicans by secondary metabolites of an endophytic fungus Alternaria tenuissima having broad spectrum antifungal potential. Microbiological Research232, 126386.
Conway, D. P., & McKenzie, M. E. (2007). Poultry coccidiosis: diagnostic and testing procedures. John Wiley & Sons.
Fakae, L. B., Stevenson, C. W., Zhu, X. Q., & Elsheikha, H. M. (2020). In vitro activity of Camellia sinensis (green tea) against trophozoites and cysts of Acanthamoeba castellaniiInternational Journal for Parasitology: Drugs and Drug Resistance13, 59-72.
Fatemi, A., Razavi, S. M., Asasi, K., & Goudarzi, M. T. (2015). Effects of Artemisia annua extracts on sporulation of Eimeria oocysts. Parasitology Research114(3), 1207-1211.
Gadelhaq, S. M., Arafa, W. M., & Abolhadid, S. M. (2018). In vitro activity of natural and chemical products on sporulation of Eimeria species oocysts of chickens. Veterinary Parasitology251, 12-16.
Gottardi, W., & Block, S. S. (1991). Disinfection, sterilization and preservation. Lea & Febiger: Philadelphia, 152-165.
Hajihossein, R., Eslamirad, Z., Rafiei, F., Naderi, G., & Assadi, M. (2020). Anti-Acanthamoeba effect of Camellia sinensis extract (black and green tea) in vitroJournal of Medicinal Plants, 19(73), 163-169. (In Persian)
Inacio, J. D., Gervazoni, L., Canto-Cavalheiro, M. M., & Almeida-Amaral, E. E. (2014). The effect of (-)-epigallocatechin 3-O-gallate in vitro and in vivo in Leishmania braziliensis: involvement of reactive oxygen species as a mechanism of action. PLoS Neglected Tropical Diseases8(8), e3093.
Isakakroudi, N., Talebi, A., Allymehr, M., & Tavassoli, M. (2018). Effects of essential oils combination on sporulation of turkey (Meleagris gallopavo) Eimeria oocysts. Archives of Razi Institute73(2), 113-120.
Jitviriyanon, S., Phanthong, P., Lomarat, P., Bunyapraphatsara, N., Porntrakulpipat, S., & Paraksa, N. (2016). In vitro study of anti-coccidial activity of essential oils from indigenous plants against Eimeria tenellaVeterinary Parasitology228, 96-102.
Moazeni, M., & Khademolhoseini, A. A. (2016). Ovicidal effect of the methanolic extract of ginger (Zingiber officinale) on Fasciola hepatica eggs: an in vitro study. Journal of Parasitic Diseases40(3), 662-666.
Molan, A. L., Liu, Z., & De, S. (2009). Effect of pine bark (Pious radiata) extracts on sporulation of coccidian oocysts. Folia Parasitologica (Prague), 56(1), 1.
Ola-Fadunsin, S. D., & Ademola, I. O. (2014). Anticoccidial effects of Morinda lucida acetone extracts on broiler chickens naturally infected with Eimeria species. Pharmaceutical Biology, 52(3), 330-334.
Pieri, F. A., Silva, V. O., Vargas, F. S., Veiga Junior, V. F., & Moreira, M. A. S. (2014). Antimicrobial activity of Copaifera langsdorffii oil and evaluation of its most bioactive fraction against bacteria of dog's dental plaque. Pakistan Veterinary Journal, 34(2).
Prasanth Reddy, V., Ravi Vital, K., Varsha, P. V., & Satyam, S. (2014). Review on Thymus vulgaris traditional uses and pharmacological properties. Medicinal and Aromatic Plants, 3(164), 2167-0412.
Raeisi, M., Safamehr, A., Khodaei Ashan, S., & Habibi, R. (2015). Thyme (Thymus vulgaris L.) and Oregano (Oreganum vulgare L.) essential oils for broilers: effect on performance, antioxidant indices and blood biochemical parameters. Animal Sciences Journal, 27(105), 103-120. (in Persian)
Razmi, G. R., & Kalideri, G. A. (2000). Prevalence of subclinical coccidiosis in broiler-chicken farms in the municipality of Mashhad, Khorasan, Iran. Preventive Veterinary Medicine, 44(3-4), 247-253.
Shirley, M. W. (1992). Research on avian coccidia: an update. British Veterinary Journal148(6), 479-499.
Shirley, M. W., Smith, A. L., & Tomley, F. M. (2005). The biology of avian Eimeria with an emphasis on their control by vaccination. Advances in Parasitology60, 285-330.
Shivaramaiah, C., Barta, J. R., Hernandez-Velasco, X., Téllez, G., & Hargis, B. M. (2014). Coccidiosis: recent advancements in the immunobiology of Eimeria species, preventive measures, and the importance of vaccination as a control tool against these Apicomplexan parasites. Veterinary Medicine: Research and Reports5, 23.
Sigei, E. C., Muturi, M., & Bii, C. (2015). Antifungal activities of Camellia sinensis crude extract, mixture with milk, on selected pathogenic and mycotoxic fungi. Journal of Medicinal Plants Research9(42), 1070-1080.
Williams, R. B. (1997). Laboratory tests of phenolic disinfectants as oocysticides against the chicken coccidium Eimeria tenellaThe Veterinary Record141(17), 447-448.