Performance, carcass quality and intestinal morphology of broilers, fed either raw or ‎fermented wheat, with Bacillus subtilis & Lactobacillus plantarum

Document Type : Research Paper

Authors

1 Former M.Sc. Student, Faculty of Agriculture, University of Guilan, Rasht, Iran‎

2 Professor, Faculty of Agriculture, University of Guilan, Rasht, Iran‎

3 Assistant Professor, International Sturgeon Research Institute, Rasht, Iran‎

4 Associate Professor, Faculty of Agriculture, University of Guilan, Rasht, Iran‎

Abstract

This study was performed to compare ordinary wheat with fermented wheat using Bacillus subtilis & Lactobacillus plantarum, and the effect of replacing different levels of fermented wheat with corn grain on performance, carcass quality and jejunom morphology in broilers. A total of 280 broilers (Ross 308) in a completely randomized design wre assigned to 7 experimental groups with 4 replicates . The experimental diets included: control diet based on corn and soybean meal and six other diets containing 20%, 30% and 40% raw wheat grain instead of corn (W40 & W20, W30) or 20%, 30% and 40% fermented wheat instead of corn (FW30 and FW40, FW20). The results showed that fermented wheat grain at the level of 40% caused a significant increase in daily weight in the starter and growth periods (P<0.05). The fermentation process led to a decrease in phytic acid and an increase in the calcium and phosphorus numerically. Villi length in the FW40 treatment was significantly increased compared to that in the control treatment (P<0.05). Breast muscle in the FW40 treatment showed a significant increase (P<0.05), when compared to treatments FW30, W40, and control. In conclusion, the use of 40% of fermented wheat grains improves the feed conversion ratio and increases the percentage of breast muscle meat.

Keywords


  1. Amerah, A. M. (2015). Interactions between wheat characteristics and feed enzyme supplementation in broiler diets. Animal Feed Science and Technology, 199, 1-9.
  2. AOAC. (2006). Official methods of analysis of the AOAC. (15th ed.). Methods 932.06, 925.09, 985.29, 923.03. Association of official analytical chemists. Arlington, VA, USA. 70-80.
  3. Barbosa, T.M., Serra, C.R., Laragione, R.M., Woodward, M.J. & Henriques,  A.O. (2005). Screening for bacillus isolates in the broiler gastrointestinal tract. Applied and Environmental Microbiology, 71, 968-978.
  4. Chen, K. L., Kho, W. L., You, S., Yeu, H. R. H., Tang, S. W. & Hsieh, C. W. (2009). Effects of Bacillus subtilis var. natto and Saccharomyces cerevisiae mixed fermented feed on the enhanced growth performance of broilers. Poultry Science, 88, 309-315.
  5. Deschepper, K., Lippens, M., Huyghebaert, G. & Molly, K. (2003). The effect of aromabiotic and GALI D’OR on technical performances and intestinal morphology of broilers. In: Proccedings of 14th European Symposium on poultry nutrition. August. Lillehammer, Norway. 189.
  6. Dibner, J. J. & Buttin, P. (2002). Use of organic acids as a model to study the impact of gut microfloraon nutrition and metabolism. Journal of Applied Poultry Research, 11, 453-463.
  7. Fallah, M., Dastar, B., Ganji, F. & Ashayerizadeh, A. (2014) . Effect of fermented soybean meal and dietary protein level on performance and gastrointestinal microbial population in broilers chicken. Animal Sciences Journal, 109, 66-53.
  8. Febles, C. I., Arias, A., Hardisson, A., Rodrı´guez-Alvarez, C. & Sierra, A. (2002) . Phytic Acid level in wheat flours. Journal Cereal Science, 36, 19-23.
  9. Feng, J., Liu, X.,  Xu, Z. R., Lu, Y. P. & Liu, Y. Y. (2007b). Effect of fermented soybean meal on intestinal morphology and digestive enzyme activities in weaned piglets. Digestive Diseases and Sciences, 52, 1845-1850.
  10. Feng, J., Liu, X., Xu, Z. R., Liu, Y. Y. & Lu, Y. P. (2007a). Effects of Aspergillus oryzae 3.042 fermented soybean meal on growth performance and biochemical parameters in broilers. Animal Feed Science and Technology, 134, 235-242.
  11. Godoy, M. G., Amorim, G. M., Barreto, S. M. & Freire, D. M. G. (2018). Agricultural residues as animal feed: protein enrichment and detoxification using solid-state fermentation. (ed). Pandey, A., Larroche, C. and Soccol, C. R. Current Developments in Biotechnology and Bioengineering. Chapter, 12, 236-256.
  12. Jafari, Z. & Najafpour, Gh. )2014(. Production of alkaline protease enzyme from Bacillus subtilis using starch effluent. M.Sc. Thesis Faculty of Chemistry Babol University of Technology.
  13. Johnson, E. A. (2013). Biotechnilogy of non-saccharomyces yeasts-the basidiomycetes. Applied Microbiology and Biotechnology, 97, 503-517.
  14. KamaliShahri, M., Najafi, M. A. & AtaSalehi, I. (2016). The influence of alone and starter culture combinations Saccharomyces cerevisiae (PTCC5052) Lactobacillus Plantarum (PTCC1058), fermentation time and temperature on phytic acid content of Wheat bran. Innovative Food Technologies, 14, 33-41.
  15. Klein Donald, W. & Lansing Harley, M. (2006). Microbiology. (6th ed.). McGraw Hill Science, Engineering and Math Hardcover. 32-48.
  16. Malekzadeh, M. & Shakouri, M. D. )2016(. The effect of four barley cultivars in whole and ground forms on performance, nutrients digestibility and blood lipid parameters of broiler chickens. Research on Animal Production, 7, 40-48.
  17. Manini, F., Poutanen, K., Brasca, M., Erba, D. & Plumed-Ferrer, C. (2016). Characterization of lactic acid bacteria isolated from wheat bran sourdough. Food Science Technology, 66, 275-283.
  18. Mathivanan, R., Selvaraj, P. & Nanjappan, K. (2006). Feeding of fermented soybean meal on broiler performance. Internatinal Journal of Poultry Science, 5, 868-872.
  19. Ross 308 Broiler Management Hand Book. (2014).
  20. Ross 308 Broiler Nutrition Specification. (2014).
  21. SAS Institute. (2003). SAS/STAT software version 9. SAS Inst. Inc., Cary, NC.
  22. Shavoli, M. & Dosti, A. (2015). Construction of glucanase gene of Bacillus subtilis in Escherichia coli. M.Sc. thesis. Faculty of Basic Sciences Islamic University of Shahrekord.
  23. Shi, C., Zhang, Y., Lu, Z. & Wang, Y. (2017). Solid-state fermentation of corn-soybean meal mixed feed with Bacillus subtilis and Enterococcus faecium for degrading antinutritional factors and enhancing nutritional value. Journal of Animal Science and Biotechnology, 8, 50-62.
  24. Skrede, G., Herstad, O., Sahlstrøm, S., Holck, A., Slinde, E. & Skrede, A. (2003). Effects of lactic acid fermentation on wheat and barley carbohydrate composition and production performance in the chicken. Animal Feed Science and Technology, 105, 135-148.
  25. Slominski, B. A. (2011). Recent advances in research on enzymes for poultry diets. Poultry Science, 90, 2013-2023.
  26. Sugiharto, S. & Ranjitka, S. (2019). Recent advances in fermented feeds towards improved broiler chicken performance, gastrointestinal tract microecology and immune responses: A review.  Animal Nutrition, 5(1), 1-10.
  27. Summers, J. & Leeson, S. (1997). Commercial Poultry Nutrition. (3rd ed.). 30-45.
  28. The National Standard Organization of Iran. (1998). Instruction. 37, 1-39.
  29. Wang, L. C., Wen, C., Jiang, Z. Y. & Zhou, Y. M. (2012). Evaluation of the partial replacement of high-protein feedstuff with fermented soybean meal in broiler diets. Journal of Applied Poultry Research, 4, 849-855.
  30. Wang, L., Zhou, H., He, R., Xu, W., Mai, K. & He, G. (2016). Effects of soybean meal fermentation by Lactobacillus plantarum P8 on growth, immune responses, and intestinal morphology in juvenile turbot (Scophthalmus maximus L.). Aquaculture, 464, 87-94
  31. Wang, Y., Deng, Q., Song, D., Wang, W., Zhou, H., Wang, L. &  Li, A. (2017). Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers. Food and Agricultural Immunology,  28, 725-738.
  32. Xu, F. Z., Zeng, X. G. & Ding, X. L. (2012). Effects of replacing soybean meal with fermented rapeseed meal on performance, serum biochemical variables and intestinal morphology of broilers. Asian Australian Journal Animal Science, 12, 1734-1741.
  33. Yasar, S., Sacid gok, M. & Gurbuz, Y. (2016). Performance of broilers fed raw or fermented and redried wheat, barley, and oat grains. Turkish Journal of Veterinary and Animal Sciences, 40, 313-322.
  34. Zhao, C. J., Schieber, A.  & Gänzle, M. G. (2016). Formation of taste-active amino acids, amino acid, amino acid derivatives and peptides in food fermentations-a review. Food Resarch International, 89, 39-47.