Effects of in ovo injection of different manganese sources on performance and tibia characteristics of broilers

Document Type : Research Paper


1 Uni. of Guilan/Member of Academic Staff +Guilan Science and Tehnology park/ Director

2 Ph.D. Student -Department of Animal science, Faculty of agriculture, University of Guilan

3 Member of Academic staff- Univesity of Guilan Faculty of Agriculture, Department of Animal Sciences

4 Institute of Medical Advanced Technologies , Guilan University of Medical Sciences


In order to investigate the effects of in ovo injection of different sources of manganese on performance and tibia characteristics of broilers, a total of 480 fertile eggs from ROSS 308 were assigned to 6 treatments, 4 replicates of 20 eggs per replicate in a completely randomized design. The experimental treatments included non-injected (negative control, NC), injection of1 ml serum physiology(positive control, PC), 1 ml of manganese sulfate (MnS), 1 ml of manganese- methionine (MnM), 1 ml of nano manganese (NMn) and 1 ml of nano manganese methionine (NMnM). The results showed that the in ovo injection of different sources of manganese lead to improvement in broilers growth. The highest and lowest body weight gain in entire production period were recorded for NMn and PC group, respectively (P<0.05). NMn and NMnM groups showed the best feed conversion ratio in whole experimental period (P<0.05). The tibia length at 21 day of age was significantly higher in treatment containing MnS and fresh and dry weights of tibia at 21 and 42 days of age were significantly increased with NMn (P<0.05). The tibia breaking strength at 21 and 42 days of age was significantly increased in treatments containing NMnM and NMn (P<0.05). The tibia volume, density and ash percentage were not affected by experimental treatments on 21 and 42 days of age (P>0.05). According to these results, it seems that the in ovo injection of manganese nanoparticles as a feed additive has positive effects on performance and tibia characteristics of broilers.


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