Effect of different dietary carbohydrate sources on in vitro utilization efficiency of corn steep liquor

Document Type : Research Paper


1 Ph.D. Candidate, Department of Animal Science, Faculty of Agricuture, Lorestan University, Khorramabad, Iran

2 Associate Professor, Department of Animal Science, Faculty of Agricuture, Lorestan University, Khorramabad, Iran

3 Assistant Professor, Department of Animal Science, Faculty of Agricuture, Lorestan University, Khorramabad, Iran


This study was carried out to evaluate the effects of supplementing corn steep liquor (CSL), as a nitrogen source, with different dietary carbohydrate sources on in vitro gas production, fermentation parameters, digestibility and activity of rumen microbial enzymes of male lamb's diets. The 8 experimental diets contained different energy sources (including different levels of barley, corn and molasses), in which a constant level of CSL was used (13% of diet dry matter (DM)), incubated in vitro. Experimental diets were 1) corn, 2) barley, 3) mixture of corn/barley and replacing the former with4) 5, 5) 10, 6) 15, 7) 20 and 8) 25% molasses. The maximum and minimum gas production at 72 and 96 (h) of incubation, and potential of gas production (b) were obtained by incubation of diet containing15% molasses and corn diets, respectively (P<0.05). However, the greatest and lowest values for fractional rate of gas production (c) and microbial protein synthesiswere observed in the diets containing 10 and 25% molasses, respectively (P<0.05). The concentration of NH3-N was greatest by supplementing CSL with 25% molasses, while it was lowest in the diet containing 10% molasses (P<0.05). The addition 10% molasses along with CSL increased the activity of carboxymethyle cellulase and microcrystalline cellulase compared with the other treatments (P<0.05). The highest activity of filter paper-degrading (FDP) was obsereved with the diet in which CLS was supplemented with 5% molasses, while supplementing CSL conating diet with 25% molasses led to the lowest FPD activity (P<0.05). In conclusion, the results of present study showed that supplementing CSL containing diet with 10% of molasses improved nutrient digestion, rumen fermentation and nitrogen metabolism in vitro.


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