Evaluation of production, biochemical, immunity and antioxidant parameters in Hy-Line W- 36 laying hens fed diets containing growth promoter antibiotic and feed additive mixture

Document Type : Research Paper


1 Associate Professor, Department of Animal Science, Faculty of Agricultural Sciences, Malayer University, Malayer, Iran

2 Former M.Sc. Student, Department of Animal Science, Faculty of Agricultural Sciences, Malayer University, Malayer, Iran

3 Assistant Professors, Department of Animal Science, Faculty of Agricultural Sciences, Malayer University, Malayer, Iran


This research was conducted to evaluate the effect of some feed additives compare to Flavomycin growth promoter antibiotic. Experiment was conducted in completely randomized design manner consisting 3 treatments, 5 replicates and 20 hens per each replicate using 300 White Leghorn, Hy-Line W-36 strain. The experimental treatments included: control, Flavomycin growth promoter antibiotic at 0.05% dietary level and feed additive mixture consisting of thyme and oregano plant extracts and propionic acid and zinc supplement at 0.1% dietary level which fed to laying hens for 42 days (3 periods of two weeks each). The results of this study showed that the utilization of feed additive mixture significantly (P<0.05) improved feed conversion ratio, egg production percentage and blood serum antibody titers against avian Influanza and low-density lipoprotein compare to control group. The blood serum antioxidant indices including Selenium, vitamin E, Superoxide dismutase and Glutathione peroxidase were significantly (P<0.05) enhanced in laying hens fed diet containing feed additive mixture in comparison with two other treatment groups. The experimental treatments had no significant effect on egg qualitative traits and blood biochemical parameters with the exception of low-density lipoprotein. According to the results of this experiment, it seems that the studied feed additive mixture can be used as a replacement for growth promoter antibiotic in laying hen diets.


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