Efficient production and application of egg yolk antibody powder against Staphylococcus aureus

Document Type : Research Paper

Authors

1 M.Sc. Student, Department of Animal Sciences, College of Agriculture, Isfahan University of Technology, Isfahan, Iran

2 Associate Professor, Department of Animal Sciences, College of Agriculture, Isfahan University of Technology, Isfahan, Iran

3 Professor, Department of Animal Sciences, College of Agriculture, Isfahan University of Technology, Isfahan, Iran

Abstract

The aim of present experiment was to investigate the immunochemical characteristics as well as in vitro inhibitory effects of specific and nonspecific egg yolk antibody powder on Staphylococcus aureus growth, as one of the main pathogenic serotypes of Staphylococcus in human and animals. A total of 16 White Leghorn hens (Hy-Line W36) of forty weeks in age were immunized by killed sonicated vaccine after plotting the growth curve and incubating the bacteria until the end of exponential growth phase. This vaccine was prepared eggs were collected daily for 8 weeks, IgY was then isolated and lyophilized by Polson method. The immunochemical properties and growth inhibitory effects of specific and nonspecific sIgY were tested against Staphylococcus aureus. The results showed that the plasma and egg specific activity of IgY against Staphylococcus aureus markedly increased after the first immunization and remained on the highest titer throughout the experimental period (P<0.01). Total IgY concentration and purity of specific IgY powder were 71% higher rather than the non-specific IgY powder (P<0.05). The administration of specific IgY powder at 100 and 200 mg significantly (P<0.05) reduced the bacterial counts during the incubation hours of 4 and 6. Compared to control group, using the highest level of specific IgY could significantly decrease 1.20 log CFU/mL (P<0.01). Therefore, the present findings indicated that Staphylococcus aureus accompanying with Freund’s adjuvant (complete form in the first immunization and incomplete form in booster immunization) have a notable potential to produce high antibody levels for a long time period. In addition, Polson method is an effective way to obtain remarkable purity for IgY with high antibacterial efficiency and long term production.

Keywords

References

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History

  • Receive Date: 07 March 2018
  • Revise Date: 07 May 2018
  • Accept Date: 14 May 2018

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