Iranian Journal of animal Science

Iranian Journal of animal Science

The effect of addition of different levels of permeable and non-permeable cryoprotectants on freezing capacity of stallions sperm

Document Type : Research Paper

Authors
Farhad Vafayi 1
ahmad zareh
Hamid Kohram 2
Afshin Seifi-jamadi 3
1 M.Sc. Student, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Associate Professor, Facility members of Razi Vaccine and Serum Research Institute, Karaj, Iran
3 ssistant Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
10.22059/ijas.2017.214312.653465
Abstract
The aim of this study was to evaluate the effect of addition of three different permeable (Glycerol, Dimethyl formamide and Dimethyl acetamid) and two different non-permeable cryoprotectant (Trehalose and sucrose) to the extender on freezing capacity of Turkmen stallions’ sperm. Four healthy mature Turkmen stallions (8-10 years old) were used. Collected semen from stallions were processed and pooled after gel removal and before allocating to treatments [glycerol, dimethyl formamide and dimethyl acetamid (5 or 7 percent) and trehalose or sucrose (50 and 100 mM)]. The prepared treatments were frozen according to a standard method. After thawing viability and motility of all treatments were accessed.The results showed that the viability and motility of spermatozoa treated with extender containing 5 or 7 % dimethyl formamide (68.6 ± 0.93 and 63.2 ± 2.22 respectively) or dimethyl acetamid (68.6 ± 1.69 and 63.6 ± 2.76 respectively) were significantly higher than those which treated with 5 or 7 %  glycerol (56.8 ± 3.06 and 55.6 ± 1.03 respectively) (P<0.05). Also, results showed that the viability and motility of stallion spermatozoa were not influenced by the non-permeable cryoprotectants (P<0.05).We concluded that dimethyl acetamid and dimethyl formamide can be proper alternatives for glycerol as a common stallion spermatozoa cryoprotectant and the sucrose and trehalose had no significant effect on the stallion sperm quality after freeze-thawing process.
Keywords
CASA
cryoprotectant
extender
Sperm
stallion
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  • Receive Date 01 October 2016
  • Revise Date 28 May 2017
  • Accept Date 28 May 2017