Effect of in ovo injection of different ratios of L-arginine to L-lysine on body growth, muscle production, and blood metabolites concentration of day old Ross broiler chicks

Document Type : Research Paper


1 M.Sc. Student, Department of Animal Science, Faculty of Agriculture, University of Tabriz, Iran

2 Assistant Professor, Department of Animal Science, Faculty of Agriculture, University of Tabriz, Iran

3 Professor, Department of Animal Science, Faculty of Agriculture, University of Tabriz, Iran


The aim of this research was to evaluate the effect of in ovo injection of different ratios of L- arginine to L- lysine on body growth, muscle production and blood metabolites concentration of day old Ross broilers chicks. A total of 210 fertile Ross 308 broiler breeder eggs were used in a completely randomized design with seven experimental groups of 30 individual eggs in each treatment. The experimental treatments included: sham-control (sterile distilled water injected), control (non-injected), and injection of solutions with 75.7, 80.7, 85.7, 90.7 and 95.7 percent L-arginine to L-lysine ratios. On hatching, the chicks were weighed and their blood samples were collected. Then, chicks were euthanized and their carcass traits were weighed. According to the results of this study, the maximum hatchability percentage was observed in 90.7 percent of L- arginine to L- lysine ratio. The results also showed that the in ovo injection of different L- arginine to L- lysine ratio up to 90.7 percent resulted in significant increase in chick weight (P< 0.01), relative weight of scalped carcass (P< 0.01), carcass efficiency (P<0.01), thigh relative weight (P< 0.05), breast muscle relative weight (P< 0.01) and relative weight of liver (P< 0.05), whereas blood plasma urea concentration was significantly (P< 0.05) decreased. In overall, results indicated that in ovo injection of 90.7% L-arginine to L-lysine ratio is an advisable ratio for improving muscle growth, blood serum metabolite concentrations, and hatchability percentage of broilers.


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