Iranian Journal of animal Science

Iranian Journal of animal Science

Effect of in ovo injection of different ratios of L-arginine to L-lysine on body growth, muscle production, and blood metabolites concentration of day old Ross broiler chicks

Document Type : Research Paper

Authors
Farideh Abdolalizadeh Alvanegh 1
Marziyeh Ebrahimi 2
Hossein Daghigh Kia 3
1 M.Sc. Student, Department of Animal Science, Faculty of Agriculture, University of Tabriz, Iran
2 Assistant Professor, Department of Animal Science, Faculty of Agriculture, University of Tabriz, Iran
3 Professor, Department of Animal Science, Faculty of Agriculture, University of Tabriz, Iran
10.22059/ijas.2017.221665.653484
Abstract
The aim of this research was to evaluate the effect of in ovo injection of different ratios of L- arginine to L- lysine on body growth, muscle production and blood metabolites concentration of day old Ross broilers chicks. A total of 210 fertile Ross 308 broiler breeder eggs were used in a completely randomized design with seven experimental groups of 30 individual eggs in each treatment. The experimental treatments included: sham-control (sterile distilled water injected), control (non-injected), and injection of solutions with 75.7, 80.7, 85.7, 90.7 and 95.7 percent L-arginine to L-lysine ratios. On hatching, the chicks were weighed and their blood samples were collected. Then, chicks were euthanized and their carcass traits were weighed. According to the results of this study, the maximum hatchability percentage was observed in 90.7 percent of L- arginine to L- lysine ratio. The results also showed that the in ovo injection of different L- arginine to L- lysine ratio up to 90.7 percent resulted in significant increase in chick weight (P< 0.01), relative weight of scalped carcass (P< 0.01), carcass efficiency (P<0.01), thigh relative weight (P< 0.05), breast muscle relative weight (P< 0.01) and relative weight of liver (P< 0.05), whereas blood plasma urea concentration was significantly (P< 0.05) decreased. In overall, results indicated that in ovo injection of 90.7% L-arginine to L-lysine ratio is an advisable ratio for improving muscle growth, blood serum metabolite concentrations, and hatchability percentage of broilers.
Keywords
Broilers
In ovo injection
L-arginine to L-lysine ratio
Muscle growth
  1. Al-Daraji, H. J., Al-Mashadani, A. A., Al-Hayani, W. K., Al-Hassani, A. S. & Mirza, H. A. (2012). Effect of in ovo injection with L-arginine on productive and physiological traits of Japanese quail. South African Journal of Animal Science, 42, 139-145.‏
  2. Al-Murrani, W. K. (1982). Effect of injecting amino acids into the egg on embryonic and subsequent growth in the domestic fowl. British Poultry Science, 23, 171-174.‏
  3. Arslan, C., Citil, M. & Saatci, M. (2004). Effect of L-Carnitine administration on growth performance, carcass traits, serum lipids and abdominal fatty acid compositions of geese. Revue de Médecine Vétérinaire, 155, 315-320.‏
  4. Asmawat, I., Sonjaya, H., Natsir, A. & Pakiding, W. (2015). Native chicken embryo quality improvement through in ovo feeding. Asian Journal of Microbiology Biotechnology & Environmental Sciences, 17, 69-74.
  5. Austic, R. E. & Nesheim, M. C. (1970). Role of kidney arginase in variations of the arginine requirement of chicks. Journal of Nutrition, 100, 855-868.
  6. Baker, D. H. (2009). Advances in protein– amino acid nutrition of poultry. Amino acids, 37, 29-41.‏
  7. Ball, R. O., Urschel, K. L. & Pencharz, P. B. (2007). Nutritional consequences of interspecies differences in arginine and lysine metabolism. The Journal of Nutrition, 137, 1626S-1641S.‏
  8. Balnave, D. & Barke, J. (2002). Re-evaluation of the classical dietary arginine: lysine interaction for modern poultry diets: a review. World's Poultry Science Journal, 58, 275-289.‏
  9. Barbul, A. (1986). Arginine: biochemistry, physiology, and therapeutic implications. Journal of Parenteral and Enteral Nutrition, 10, 227-238.‏
  10. Bautista- Ortega, J. & Ruiz- Feria, C. A. (2010). L-Arginine and antioxidant vitamins E and C improve the cardiovascular performance of broiler chickens grown under chronic hypobaric hypoxia. Poultry Science, 89, 2141-2146.‏
  11. Bhanja, S. K. & Mandal, A. B. (2005). Effect of in ovo injection of critical amino acids on pre and post hatch growth, immunocompetence and development of digestive organs in broiler chickens. Asian-Australasian Journal of Animal Science, 18, 524-531.
  12. Bhanja, S. K., Baran Mandal, A., Agarwal, S. K. & Majumdar, S. (2012). Modulation of post hatch-growth and immunocompetence through in ovo injection of limiting amino acids in broiler chickens. Indian Journal of Animal Sciences, 82, 993-8.
  13. Davis, S. L. (1972). Plasma levels of prolactin, growth hormone, and insulin in sheep following the infusion of arginine, leucine and phenylalanine. Endocrinology, 91, 549-555.‏
  14. Deng, K., Wong, C. W. & Nolan, J. V. (2005). Long- term effects of early life L- arginine supplementation on growth performance, lymphoid organs and immune responses in Leghorn- type chickens. British Poultry Science, 46, 318-324.‏
  15. Ebrahimi, M., Janmohammadi, H., Daghigh Kia, H., Moghaddam, G., Rajabi, Z., Rafat, S. A. & Javanmard A. (2017). The effect of L-lysine in ovo feeding on body weight characteristics and small intestine morphology in a day-old Ross broiler chicks. Revue de Médecine Vétérinaire, 168, 116-124.
  16. Ebrahimi, M., Zare Shahneh, A., Shivazad, M., Ansari Pirsaraei, Z., Tebianian, M., Adibmoradi, M. & Nourijelyani, K. (2013). Evaluation of the effect of feeding L- arginine on growth performance, carcass traits and blood parameters in broiler chickens. Iranian Journal of Animal Science, 44, 157-166.
  17. Ebrahimi, M., Zare Shahneh, A., Shivazad, M., Ansari Pirsaraei, Z., Tebianian, M., Adibmoradi, M. & Nourijelyani, K. (2014). The effects of dietary L- arginine on growth, meat production, and fat deposition in broiler chickens. Iranian Journal of Animal Science Research, 5, 281-290.
  18. Edwards, N. M., Heberle, N. D. & Hynd, P. I. (2016). The effect of in ovo administration of L-arginine on the hatchability and embryological development of broiler chicks. SAP Animal Production 2016, Adelaide.
  19. Emadi, M., Kaveh, K., Bejo, M. H., Ideris, A., Jahanshiri, F., Ivan, M. & Alimon, R. A. (2010). Growth performance and blood parameters as influenced by different levels of dietary arginine in broiler chickens. Journal of Animal and Veterinary Advances, 9, 70-74.‏
  20. Fernandes, J. I. M., Murakami, A. E., Martins, E. N., Sakamoto, M. I. & Garcia, E. R. M. (2009). Effect of arginine on the development of the pectoralis muscle and the diameter and the protein: deoxyribonucleic acid rate of its skeletal myofibers in broilers. Poultry Science, 88, 1399-1406.‏
  21. Floyd Jr., J. C., Fajans, S. S., Conn, J. W., Knopf, R. F. & Rull, J. (1966). Stimulation of insulin secretion by amino acids. Journal of Clinical Investigation, 45, 1487- 1502.‏
  22. Foye, O.T., Uni, Z., McMurty, J.P. & Freket, P.R. (2006). The effects of nutrient administration, "In ovo feeding" of Arginine and/or β-hydroxy-β-methyle butyrate (HMB) on insulin–like growth factors, energy metabolism and growth in turkey poults. International Journal of Poultry Science, 5, 309-317.
  23. Hall, J. E. (2015). Guyton and Hall Textbook of Medical Physiology. Elsevier Health Sciences.‏ 13th ed.‏ Philadelphia, PA.
  24. Jobgen, W. S., Fried, S. K., Fu, W. J., Meininger, C. J. & Wu, G. (2006). Regulatory role for the arginine-nitric oxide pathway in metabolism of energy substrates. The Journal of Nutritional Biochemistry, 17, 571-588.‏
  25. Khajali, F. & Wideman, R. F. (2010). Dietary arginine: metabolic, environmental, immunological and physiological interrelationships. World's Poultry Science Journal, 66, 751-766.‏
  26. Kita, K., Kato, S., Yaman, M. A., Okumura, J. & Yokota, H. (2002). Dietary L-carnitine increases plasma insulin-like growth factor-I concentration in chicks fed a diet with adequate dietary protein level. British Poultry Science, 43, 117-121.‏
  27. Labadan, M. C., Hsu, K. N. & Austic, R. E. (2001). Lysine and arginine requirements of broiler chickens at two-to three-week intervals to eight weeks of age. Poultry Science, 80, 599-606.‏
  28. Le Roith, D., Bondy, C., Yakar, S., Liu, J. L. & Butler, A. (2001). The somatomedin hypothesis. Endocrine Reviews, 22, 53-74.‏
  29. Lorenzoni, A. G. & Ruiz- Feria, C. A. (2006). Effects of vitamin E and L-arginine on cardiopulmonary function and ascites parameters in broiler chickens reared under subnormal temperatures. Poultry Science, 85, 2241-2250.‏
  30. Nayak, N., Rajini, R. A., Ezhilvalavan, S., Sahu, A. R. & Kirubaharan, J. J. (2016). Influence of in-ovo arginine feeding on post-hatch growth performance and economics of broilers. Journal of Animal Science, 6, 585-591.‏
  31. Ohta, Y., Tsushima, N., Koide, K., Kidd, M. T. & Ishibashi, T. (1999). Effect of amino acid injection in broiler breeder eggs on embryonic growth and hatchability of chicks. Poultry Science, 78, 1493-1498.
  32. Popovic, P. J., Zeh, H. J. & Ochoa, J. B. (2007). Arginine and immunity. The Journal of Nutrition, 137, 1681S-1686S.‏
  33. SAS Institute Inc. (2008). SAS/STAT User's Guide, Version 9.2. Cary, NC: SAS Institute Inc.
  34. Selle, P. H., Ravindran, V., Ravindran, G. & Bryden, W. L. (2007). Effects of dietary lysine and microbial phytase on growth performance and nutrient utilisation of broiler chickens. Asian- Australasian Journal of Animal Sciences, 20, 1100-1107.‏
  35. Smith, J. H. (1963). Relation of body size to muscle cell size and number in the chicken. Poultry Science, 42, 283-290.‏
  36. Taboada, M. C., Rodriguez, B., Millan, R. & Miguez, I. (2006). Role of dietary L-arginine supplementation on serum parameters and intestinal enzyme activities in rats fed an excess- fat diet. Biomedicine and Pharmacotherapy, 60, 10-13.‏
  37. Tesseraud, S., Maaa, N., Peresson, R. & Chagneau, A. M. (1996). Relative responses of protein turnover in three different skeletal muscles to dietary lysine deficiency in chicks. British Poultry Science, 37, 641-650.‏
  38. Uni, Z. & Ferket, R. P. (2004). Methods for early nutrition and their potential. World's Poultry Science Journal, 60, 101-111.‏
  39. Wideman Jr., R. F. & Bottje, W. G. (2000). U.S. Patent No. 6,127,421. Washington, DC: U.S. Patent and Trademark Office.‏
  40. Zhao, J. P., Jiao, H. C., Song, Z. G. & Lin, H. (2009). Effects of L-arginine supplementation on glucose and nitric oxide (NO) levels and activity of NO synthase in corticosterone- challenged broiler chickens (Gallus gallus). Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 150, 474-480.‏
Iranian Journal of animal Science
Volume 48, Issue 2 - Serial Number 2
Summer 2017
Pages 207-217

  • Receive Date 05 December 2016
  • Revise Date 22 June 2017
  • Accept Date 10 July 2017