Effect of fiber-rich molting diets on performance, gastro intestinal fermentation and microbial activity of laying hens

Document Type : Research Paper


Researcher, Department of Animal Sciences, Qazvin Agricultural and Natural Resources Research and Education Center, Qazvin-Iran


The objective of this experiment was to studythe effects of fiber-rich molting diets as alternative to the conventional feed withdrawal method, on performance, gastro intestinal fermentation and microbial activity of laying hens. One hundred eighty Hy-line (W36) laying hens were used in a completely randomized design with 5 treatments and 6 replicates by 12 birds in each replication during 12 days. Treatments were: 1- control group (hens fed with a layer diet), 2- feed withdrawal group, 3- laying hen diet containing 20000 mg zinc oxide/kg, 4- laying hen diet diluted by adding 90% alfalfa meal, 5- laying hen diet diluted by adding 90% palm kernel meal. Egg production of hen was monitored for 12 weeks during post molting period. Results show that lactobacilli and Escherichia coli bacteria population was significantly greater (P<0.05) in cecal contents of hens fed by palm kernel meal and withdrawal hens respectively than other treatments in the end of molting period. Also cecal total volatile fatty acids concentration was greater in hens fed by alfalfa than other treatments (P<0.05). The means of egg mass in fed treatments was generally higher than feed withdrawal and control treatments (P<0.05). The results suggest that fiber-rich diets compared to non-fiber diets and feed withdrawal lead to an increase in populations of useful bacteria and fermentation products and improvement of post molting production.


  1. Bach Knudsen, K.E. (2001). The nutritional significance of dietary fiber analysis. Animal Feed Science and Technology, 90, 3-20.
  2. Baurhoo, B., Letellier, A., Zhao, X. & Ruiz-Feria, A. (2007). Cecal populations of lactobacilli and bifidobacteria and eacherichia coli populations after in vivo Escherichia coli challenge in birds fed diets with purified lignin on mannanoligosaccharides. Poultry Science, 86, 2509-2516.
  3. Barnes, E.M., Impey, C.S & Cooper, D.M. (1980). Manipulation of the crop and intestinal flora of the newly hatched chick. American Journal of Clinical Nutrition, 33, 2426-2433.
  4. Berry, W.D. (2003). The physiology of induced molting. Poultry Science, 82, 971-980.
  5. Brink, M.F., Becker, D.E., Terrill, S.W. & Jensen, A.H. (1950). Zinc toxicity in the weanling pig. Journal of Animal Science, 18, 836-842.
  6. Cummings, J.H. & Macfarlane, G.T. (2002). Gastrointestinal effects of prebiotics. British Journal of Nutrition, 87, 145-151.
  7. Corrier, D.E., Nisbet, D.J., Scanlan, C.M., Hollister, A.G. & DeLoach, J.R. (1995). Control of Salmonella typhimurium colonization in broiler chicks with a continuous-flow characterized mixed culture of cecal bacteria. Poultry Science, 74, 916-924.
  8. Corrier, D.E., Nisbet, D.J., Hargis, B.M., Holt, P.S. & DeLoach, J.R. (1997). Provision of lactose to molting hens enhances resistance to Salmonella Enteritidis colonization. Journal of Food Protection, 60, 10-15.
  9. Donalson, L.M., Kim, W.K., Hererra, P., Woodward, C.L., Kubena, L.F., Nisbet, D.J. & Ricke, C.S. (2004). Combining a prebiotic with an alfalfa molting diets to increase in vitro fermentation by laying hen cecal bacteria. Poultry Science, 83(suppl. 1), 1797.
  10. Donalson, L.M., Kim, W. K., Herrera, P., Woodward, C. L., Kubena, L. F., Nisbet, D. J. & Ricke, S. C. (2005). Utilizing different ratios of alfalfa and layer ration for molt induction and performance in commercial laying hens. Poultry Science, 84, 362-369.
  11. Donalson, L.M., McReynolds, J.L., Kim, W.K., Herrera, P., Chalova, V.I., Woodward, C.L., Kubena, L.F., Nisbet, D.J. & Ricke, S.C. (2008). The influence of a fructooligosaccharide prebiotic combined with alfalfa molt diets on the gastrointestinal tract fermentation, salmonella enteritidis and intestinal shedding in laying hens. Poultry Science, 87, 1253-1262.
  12. Dunkley, C.S., McReynolds, J.L., Dunkley, K.D., Kubena, L.F., Nisbet, D.J. & Ricke S.C. (2007). Molting in salmonella enteritidis-challenged laying hens fed alfalfa crumbles. III. Blood plasma metabolite response. Poultry Science, 86, 2492-2501.
  13. Fernandez, F., Hintaon, M. & Van Gils, B. (2000). Evaluation of the effect of mannan oligosaccharides on the competitive exclusion of salmonella enteritidis colonization in broiler chicks. Avian Pathology, 29, 575-581.
  14. Fernandez, F., Hintaon, M. & Van Gils, B. (2002). Dietary mannan oligosaccharides and their effect on chicken caecal microflora in relation to salmonella enteritidis colonization. Avian Pathology, 31, 49-58.
  15. Flickinger, E.A & Fahey, G.C. (2002). Pet food and feed applications of inulin, oligofructose and other oligosaccharides. British Journal of Nutrition, 87, 297-300.
  16. Fox, M.R.S. (1989). Zinc excess. In: C.F. Mills, (Ed), Zinc excess in human biology. (pp.365-234.) Spring-Verlag, New York.
  17. Johnson, A.L & Brake, J. (1992). Zinc-induced molt: evidence for a direct inhibitory effect on granulosa cell steroidogenesis. Poultry Science, 71, 161-167.
  18. Landers, K.L., Woodward, C.L., Kubena, L.F., Nisbet, D.J. & Ricke, S.C. (2005). Alfalfa as a single dietary source for molt induction in laying hens. Bioresource Technology, 96, 565-570.
  19. Landers, K. L., Moore, R.W., Dunkley, C.S., Herrera, P., Kim, W.K., Landers, D.A., Howard, Z.R., McReynolds, J.L., Byrd, J.A., Kubena, L.F., Nisbet, D. J. & Ricke, S.C. (2007). Immunological cell and serum metabolite response of 60-week-old commercial laying hens to an alfalfa meal molt diet. Bioresource Technology, 99, 604-608.
  20. Matsushima, J.K. (1972). Feedlot Feeding. Alfalfa Science and Technology. (pp.632-640). C. H. Hanson, ed. American Society of Agronomy, Madison, WI.
  21. Moore, R.W., Park, S.Y., Kubena, L.F., Byrd, J.A., McReynolds, J.L., Burnham, M.R., Hume, M.E., Birkhold, S.G., Nisbet, D.J. & Ricket, S.C. (2004). Comparison of zinc acetate and propionate addition on gastrointestinal tract fermentation and susceptibility of laying hens to salmonella enteritids during forced molt. Poultry Science, 83, 1276-1286.
  22. Mrosovsky, N & Sherry, D.F. (1980). Animal anorexias. Science, 207, 837-842.
  23. Okumura, J., Furuse, M., Kawamura, T., Toyoshima, K. Sugawara, M., Suzuki, T., Seo, G. & Soga. H. (1994). Effects of glucooligosaccharides and bacteria on egg production rate and caecal bacteria population in the chicken. Japanese Poultry Science, 31, 189-194.
  24. Onifade, A.A. & Babatunde, G.M. (1998). Comparison of the utilisation of palm kernel meal, brewers dried grains and maize offal by broiler chicks. British Poultry Science,39, 245-250.
  25. Park, S.Y., Birkhold, S.G., Kubena, L.F., Nisbet, D.J. & Ricke, S.C. (2004). Effects of high zinc diets using zinc propionate on molt induction, organs, and post molt egg production and quality in laying hens. Poultry Science, 83, 24-33.
  26. Ponte, P.I.P., Mendes, I., Quaresma, M., Aguiar, M.N.M., Lemos, J.P.C., Ferreira, L.M.A., Soares, M.A.C., Alfaia, C.M., Prates, J.A.M & Fontes, C.M.G.A. (2004). Cholesterol levels and sensory characteristics of meat from broilers consuming moderate to high levels of alfalfa. Poultry Science, 83, 810-814.
  27. Ricke, S. (2003). The gastrointestinal tract ecology of Salmonella enteritidis colonization in molting hens. Poultry Science, 82, 1003-1007.
  28. Rijnen, M. M. J. A., Heetkemp, J. W., Verstegen, M. W. A. & Schrama, J. W. (1999). Effects of dietary fermentable carbohydrates on physical activity and energy metabolism in group-housed sows. Proceedings of the ASAS Meetings, (pp.182).
  29. SAS, Institue INC. (2008). SAS User’s Guide. Version 9.2. Statistical Analysis Systems Institue Inc., Cary, NC, USA.
  30. Sen, S., Makkar, H. P. S. & Becher. K. (1998). Alfalfa saponin and their implications in animal nutrition. Journal of Agricultural Food Chemistry, 46, 131-140.
  31. Sundu, B., Kumar, A. & Dingle, J. (2005). Comparison of feeding values of palm kernel meal and copra meal for broilers. Recent Advances in Animal Nutrition Australia, 15, 16A.
  32. Tungland, B. C & Meyer, D. (2002). Nondigestible oligo- and polysaccharides (dietary fiber): Their physiology and role in human health and food. Comprehensive. Reviews Food Science and Food Safety, 1, 73-92.
  33. Ueda, H., Takagi, A., Katou, K. & Matsumoto, S. (2002). Feeding behavior in chicks fed tea saponin and quinine sulfate. Poultry Science, 39, 34-41.
  34. Wenk, C. (2001). The role of dietary fiber in the digestive physiology of the pig. Animal Feed Science and Technolog, 90, 21-33.