Storage of rooster semen in liquid form using alcoholic extract of rosemary

Document Type : Research Paper


1 Former M. Sc. Student, Department of Animal Science, Faculty of Agriculture, University of Guilan, Iran

2 Associate Professor, Department of Animal Science, Faculty of Agriculture, University of Guilan, Iran


Current experiment was conducted to evaluate the effect of alcoholic extract of rosemary leaves on rooster sperm storage at 4 °C by using eight mature roosters. Semen collection was performed three days’ interval in 5 times. In each session, ejaculates were pooled, split into six parts and the amounts of 0 (R0), 10(R10), 20 (R20), 30 (R30), 40 (R40) and 50 µg/mL (R50) of rosemary extract were added to each part. After that, samples were chilled to 4 ℃ and kept until 72 h. Sperm viability (by staining Hoechst 33258), motility and functional membrane integrity were evaluated at 0 (T0), 24 (T24), 48 (T48) and 72 h (T72). Concentration of malondialdehyde (MDA) was determined to assay lipid peroxidation by one million spermatozoa at 48 h. The results showed that the concentration of MDA was lower in R50 (0.93 nM) than control (1.15 nM), but, it was higher than R40 (0.82 nM, P<0.05). Membrane integrity and sperm viability were higher in 40 µg/mL rosemary extract (75.72% and 73.56%, respectively) than control (70.6% and 69.08%, respectively) and 10 µg/mL extract (71.56% and 69.72%, respectively; P<0.05). There was interaction between rosemary extract and storage time on sperm motility (P<0.05). After 72 h, sperm motility was higher R40 (46%) than R0 (34%), R10 (35.6%) and R20 (38.8%, P<0.05). Therefore, rosemary extract improves quality of rooster spermatozoa during storage at 4 °C.


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