Document Type : Research Paper
Ph.D. Student, Department of Animal Science, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
Professor, Department of Animal Science, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
Professor, Department of Animal Science, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
Assistant Professor, Department of Nutrition and Physiology, Animal Science Research Institute, Karaj, Iran
Assistant Professor, Department of Animal Science, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
The aim of this study was to investigate the effect of reducing metabolizable protein with rumen-protected Lysine and Methionine in diet during the last 5 weeks of gestation on performance and blood parameters of Afshari ewes and lambs. Twenty five mature (once lambing) ewes in a Unbalanced complete randomized design fed with diets containing low metabolizable protein but containing rumen-protected lysine and methionine (LMP + LMRP) and high (HMP), respectively, 20 percent less than and equal to recommendations of NRC (2007), The results showed no difference between the treatments regarding dry matter intake, body weight of ewes at the end of gestation, body weight changes from start of the experiment until the birth, body weight after lambing and 20 days post lambing (p>0.05). Decline changes in Body weight at lambing was increased in the HMP group rather than LMP+LMRP group and the difference was tend to significant (p
- Abdelrahman, M. M. & Hunaiti, D. A. (2007). The effect of dietary yeast and protected methionine on performance and trace minerals status of growing Awassi lambs. Journal of Livestock Science, 115, 235-241.
- Ali, C.S., Din, I., Sharif, M., Nisa, M., Javaid, A., Hashmi, N. & Sarwar, M. (2009). Supplementation of ruminally protected proteins and amino acids: feed consumption, digestion and performance of cattle and sheep. Internatinal Journal of Agriculture Biology, 11, 477-482.
- Amanlou, H., Karimi, A., Mahjoubi, E. & Milis, C. (2011). Effects of supplementation with digestible undegradable protein in late pregnancy on ewe colostrum production and lamb output to weaning. Journal of Animal Physiology and Animal Nutrition, 95, 616-622.
- Ammann, H. M. (1991). Influence of protected fat and protected protein given separately or together on physiology of digestion in the rumen, abomasum and feces of sheep. Institute Fur Tierernahrung, TierarzticheHochschule Hannover, Germany.
- Annett, R. W., Carson, A. F. & Dawson, L. E. R. (2008). Effects of digestible undegradable protein (DUP) supply and fish oil supplementation of ewes during late pregnancy on colostrums production and lamb output. Animal Feed Science and Technology,146, 270-288.
- Annett, R.W., Carson, A.F. & Dawson, L. E. R. (2005). The effect of digestible undegradable protein (DUP) content of concentrates on colostrum production and lamb performance of triplet-bearing ewes on grass-based diets during late pregnancy. Animal Science, 80, 101-110.
- Armentano, L.E., Bertics, S.J. & Ducharme, G. A. (1997). Response of lactating cows to methionine or methionine plus lysine added to high protein diets based on alfalfa and heated soybeans. Journal of Dairy Science, 80, 1194-1199.
- Bateman, H.G., Spain, J.N., Kerly, M.S., Belyea, R.L. & Marshall, R.T. (1999). Evaluation of ruminally protected methionine and lysine or blood meal and fishmeal as protein sources for lactating Holsteins. Journal of Dairy Science, 82, 2115–2120.
- Benefield, B.C., Patton, R.A., Stevenson, M.J. & Overton, T.R. (2009). Evaluation of rumen protected methionine sources and period length on performance of lactating dairy cows within Latin squares. Journal of Dairy Science, 92, 4448-4455.
- Benefield, B.C., Patton, R.A., Stevenson, M.J. & Overton, T.R. (2006). Evaluation of rumen-protected methionine (RP-Met) sources and period length on performance of lactating dairy cows within Latin squares. Journal of Dairy Science, 89 (Suppl. 1).
- Bobe, G., Young, J. W. & Beitz, D. C. (2004). Invited review: pathology, etiology, prevention, and treatment of fatty liver in dairy cows. Journal of Dairy Science, 87, 3105-3124.
- Broderick, G. A. & Reynal, S. M. (2009). Effect of source of rumen-degraded protein on production and ruminal metabolism in lactating dairy cows. Journal of Dairy Science, 92, 2822-2834.
- Broderick, G.A., Stevenson, M.J., Patton, R.A., Lobos, N.E. & Olmos Colmenero, J.J. (2008). Effect of supplementing rumen-protected methionine on production and nitrogen excretion in lactating dairy cows. Journal of Dairy Science, 91, 1092-1102.
- Broderick, G. A. (2006). Nutritional strategies to reduce crude protein in dairy diets, 21st Annual Southwest Nutrition & Management, Conference February 23-24.
- Brosnan, J. T. & Brosnan M. E. (2006). The Sulfur-Containing Amino Acids: An Overview. The Journal of Nutrition, 136, 1636S-1640S.
- Cannas, A., Tedeschi, L. O., Fox, D. G., Pell, A.N. & Van Soest, P.J. (2004). A mechanistic model to predict nutrient requirements and feed biological values for sheep. Journal of Animal Science, 82, 149-169.
- Cho, J., Overton, T. R., Schwab, C. G. & Tauer, L. W. (2007). Determining the amount of rumen-protected methionine supplement that corresponds to the optimal levels of methionine in metabolizable protein for maximizing milk protein production and profit on dairy farms. Journal of Dairy Science, 90, 4908-4916.
- Christenson, R. K. & Prior, R. L. (1976). Influence of dietary protein and energy on reproductive performance and nitrogen metabolism in Finn cross ewes. Journal of Animal Science, 43, 1104-1113.
- Davidson, S., Hopkins, B.A., Odle, J., Brownie, C., Fellner, V. & Whitlow, L.W. (2008). Supplementing limited methionine diets with rumen-protected methionine, betaine, and choline in early lactation Holstein cows. Journal of Dairy Science, 91, 1552-1559.
- Davidson, S., Hopkins, B. A., Diaz, D. A., Bolt, S. M., Brwnie, C., Fellner, V. & Witlow, L. W. (2003). Effect of Amount and Degradability of Dietary Protein on Lactation, Nitrogen Utilization, and Excretionin Early Lactation Holstein Cows. . Journal of Dairy Science, 86, 1681-1689.
- Dawson, L. E. R., Carson, A. F. & Kilpatrick, D. J. (1999). The effect of digestible undegradable protein concentration of concentrates and protein source offered to ewes in late pregnancy on colostrum production and lamb performance. Animal Feed Science and Technology, 82, 21-36.
- DeLouis, C., Djiane, J., Houdebine, L. M. & Terqui, M. (1980). Relation between hormones and mammary gland function. Journal of Dairy Science, 63, 1492-1513.
- DePeters, E. J. & Cant, J. P. (1992). Nutritional factors influencing the nitrogen composition of bovine milk: A review. Journal of Dairy Science, 75, 2043-2070.
- Doepel, L., Pacheco, D., Kennelly, J. J., Hanigan, M. D., Lopez, I. F. & Lapierre, H. (2004). Milk protein synthesis as a function of amino acid supply. Journal of Dairy Science, 87, 1279-1297.
- Drackley, J. K., Overton, T. R. & Douglas, N. (2001). Adaptations of glucose and long chain fatty acid metabolism in liver of dairy cows during the periparturient period. Journal dairy science, 84(E. Suppl.) E 100-E112.
- Fenderson, C. L. & Bergen, W. G. (1975). An assessment of essential amino acid. Journal of Animal Science, pp: 12.
- Girard, C. L., Lapierre, H., Matte, J. J. & Lobley, G. E. (2004). Effects of dietary supplements of folic acid and rumen-protected methionine on lactational performance and folate metabolism of dairy cows. Journal of Dairy Science, 88, 660-670.
- Graulet, B., Richard, C. & Robert, J. C. (2005). Methionine Availability in Plasma of Dairy Cows 230 Supplemented with Methionine Hydroxy Analog Isopropyl Ester. Journal of Dairy Science, 88, 3640-3649.
- Holter, J. B., Byrne, J. A. & Schwab, C. G. (1982). Crude Protein for High Milk Production. Journal of Dairy Science, 65, 1175-1188.
- Hatfield, P. G., Snowder, G. D., Head, J. W. A., Glimp, H. A., Stobart, R. H. & Besser, T. (1995). Production by ewes rearing single or twin lambs: effects of dietary crude protein percentage and supplemental zinc methionine. Journal of Animal Science, 73, 1227-1238.
- Henze, P., Bickhardt, K., Fuhrmann, H. & Sallmann, H. P. (1998). Spontaneous pregnancy toxemia (ketosis) in sheep and the role of insulin. Journal of Veterinary Medicine, 45, 255-266.
- Houdijk, J. G. M., Kyriazakis, I., Jackson, F., Huntley, J. F. & Coop, R. L. (2000). Can an increased intake of metabolizable protein affect the periparturient relaxation in immunity against Teladorsagia circumcincta in sheep? Veterinary parasitology, 91, 43-62.
- Illg, D. J., Sommerfeldt, J. L. & Schingoethe, D. J. (1987). Lactational and systemic responses to the supplementation of protected methionine in soybean meal diets. Journal of Dairy Science, 70, 620-629.
- Johnson, H. E., Whitehouse, N. L., Garthwaite, B. D., Piepenbrink, M. S. & Schwab, C. G. (1999). Supplementation of corn and barley-based diets of late gestation and early lactation cows with liquid methioninehydroxy analog. Journal of Dairy Science, 82(Suppl. 1), 65.
- Koenig, K. M., Rode, L. M., Knight, C. D. & Vasquez-anon, M. (2002). Rumen degradation and availability of various amounts of liquid methionine hydroxyl analog in lactating dairy cows. Journal of Dairy Science, 85, 930-938.
- KrÖber, T. F., Kreuzer, M., Senn, M., Langhans, W. & Sutter, F. (2000). Effect of rumen-protected methionine in a low protein ration on metabolic traits and performance of early lactating cows as opposed to rations with elevated crude protein content. Journal of Animal Physiology and Animal Nutriti, 8, 148-164.
- Lara, A., Mendoza, G., Landois, L., Barcena, R., Sánchez-Torres, M., Rojo, R., Ayala, J. & Vega, S. (2006). Milk production in Holstein cows supplemented with different levels of ruminally protected methionine. Livestock Science, 105, 105-108.
- Leonardi, C., Stevenson, M. & Armentano, L. E. (2003). Effect of two levels of crude protein and methionine supplementation on performance of dairy cows. Journal of Dairy Science, 86, 4033-4042.
- McMurray, C. H., Blanchflower, W. J. & Rice, D. A. (1984). Automated kinetic method for D- hydroxybutyrate in plasma or serum. Clinical chemistry, 30, 421-425.
- Mcneil, D. M., Sepetis, R., Ehrhardt, R. A., Smith, D. M. & Bell, A. W. (1997). Protein requirement of sheep in late pregnancy: partitioning of nitrogen between gravid uterus and maternal tissues. Journal of Animal Science, 75, 809-816.
- McNeill, T. H., Mori, N. & Cheng, H. W. (1999). Differential regulation of the growth-associated proteins, GAP-43 and SCG-10, in response to unilateral cortical ablation in adult rats. Neuroscience, 90, 1349-1360.
- Metges, C. C. (2001). Does dietary protein in early life affect the development of adiposity in mammals? Journal Nutrtion, 131, 2062-2066.
- Milis, C. h., Liamadis, D., Roubies, N., Christodoulou, V. & Giouseljiannis, A. (2005). Comparison of corn gluten products and a soybean-bran mixture as sources of protein for lactating Chios ewes. Small Ruminant Research, 58, 237-244.
- Mousavi, S. S., Amanlou, H., Nikkhah, A., Mirzaei Alamouti, H. R. & Tehrani, A. M. (2012). Effect of different levels balanced metabolizable protein for essential amino acids in late gestation on milk production and health status Afshari ewes. Report Research Project Agriculture Natural Resources Research and Education Center of Zanjan.
- Muniyappa, R., Lee, S. & Chen, H. (2008). Current approaches for assessing insulin sensitivity and resistance in vivo: advantages, limitations, and appropriate usage. American Journal of Physiology Endocrinology Metabolism, 294(1), E15-26.
- National Research Council. (2007). Nutrient Requirement of sheep. Sixth rev. ed. Nati. Acad. Sci. Washington DC.
- Nathalie, L. F., Delphine, M. & Christiane, O. (2004). Modifications of protein and amino acid metabolism during inflammation and immune system activation. Livestock Production Science, 87, 37-45.
- Nicols, J. R., Schingoethe, D. J., Moiga, H. A., Brouk, M. J. & Piepenbrink, M. S. (1997). Evaluation of corn distillers’ grains and ruminally protected lysine and methionine for lactating dairy caws. Journal of Dairy Science, 81, 482-491.
- Nonnecke, B.J., Foote, M.R., Smith, J.M., Pesch, B.A. & Van Amburgh, M.E. (2003). Composition and functional capacity of blood mononuclear leukocyte populations from neonatal calves on standard and intensified milk replacer diets. Journal of Dairy Science, 86, 3592-3604.
- Ocak, N., Cam, M.A. & Kuran, M. (2005). The effect of high dietary protein levels during late gestation on colostrum yield and lamb survival rate in singleton–bearing ewes. Small Ruminant Research, 56, 89-94.
- O'Doherty, J.V. & Crosby, T.F. (1997). The Effect of diet in late pregnancy on colostrum production and immunoglobulin absorption in sheep. Journal of Animal Science, 64, 87.
- .O’Doherty, J.V. & Crosby, T.F. (1996). The effect of diet in late pregnancy on progesterone concentration and colostrum yield in ewes. Theriogenology, 46, 233-241.
- Overton, T.R. (1999). Energy nutrition of transition dairy cows. Animal Science, Mimeograph series. No. 201.
- Overton, T. R., Emmert, L. S. & Clark, J. H. (1998). Effects of source of carbohydrate and protein and rumen-protected methionine on performance of cows. Journal of Dairy Science, 81, 221-228.
- Overton, T. R., LaCount, D. W., Cicela, T. M. & Clark, J. H. (1996). Evaluation of a ruminally protected methionine product for lactating dairy cows. Journal of Dairy Science, 79, 631-638.
- Patterson, M. C., Di Bisceglie, A. M., Higgins, J. J., Abel, R. B., Schiffmann, R., Parker, C. C., Argoff, C. E., Grewal, R. P., Yu, K. & Pentchev, P. G. (1993). The effect of cholesterol-lowering agents on hepatic and plasma cholesterol in Niemann-Pick disease type C. Neurology, 43, 61-4.
- Piepenbrink, M. S., Overton, T. R. & Clark, J. H. (1996). Response of cows fed a low crude protein diet to ruminally protected methionine and lysine. Journal of Dairy Science, 79, 1638-1646.
- Pisek, L., Travnicek, J., Salat, J., Kroupova, V. & Soch, M. (2008). Changes in white blood cells in sheep blood during selenium supplementation. Veterinarni Medicina. 53, 255–259.
- Pisulewski, P. M., Rulquin, H., Peyraud, J. L. & Verite, R. (1996). Lactational and systemic responses of dairy cows to postruminal infusions of increasing amounts of methionine. Journal of Dairy Science, 79, 1781-1791.
- Powell, J. M., Broderick, G. A. & Misselbrook, T. H. (2008). Seasonal diet affects ammonia emissions from tie-stall dairy barns. Journal of Dairy Science, 91, 857-869.
- Purchas, B. J., Waghorn, G. C. & Wickham G. A. (1998). Effect of pasture supplemented with methionine on wool growth and selected growth parameters. Proceedings of the New Zealand Society of Animal Production, Volume 58, pp 189-191.
- Purroy, A. & Jaime, C. (1995). The response of lactating and dry ewes to energy intake and protein source in the diet. Small Ruminant Research, 17, 17-24.
- Richardson, C. R. & Hatfield, E. E. (1978). The limiting amino acids in growing cattle. Journal of Dairy Science, 46, 740-745.
- Robinson, P.H. (1996). Rumen protected amino acids for dairy cattle: what is the future? In: Blair, R. (Ed.), Proceedings of the North American Nutrition Conferences III; Animal Feed Science Technology, 89, 51-56.
- Robinson, J. J. (1985). Nutritional requirement of the pregnant and lactating ewe. In: land, R. B., Robinson, O. W. (Eds), Genetics of Reproduction in sheep, Butterworths, London. Pp, 361-371.
- Robinson, P. H., Chalupa, W., Sniffen, C. J., Julien, W. E., Sato, H., Watanabe, K., Fujieda, T. & Suzuki, H. (1998). Ruminally protected lysine or lysine and methionine for lactating dairy cows fed a ration designed to meet requirements for microbial and postruminal protein. Journal of Dairy Science, 81, 1364-1373.
- Rulquin, H. & Delaby, L. (1997). Effects of the energy balance of dairy cows on lactation responses to rumen-protected methionine. Journal of Dairy Science, 80, 2513-2522.
- Sargison, N. D. (2007). Pregnancy toxemia. In: Diseases of Sheep. I. D. Aitken, ed. Blackwell Publishing, Oxford, UK. Pp, 359-362.
- SAS. (2004). Users Guids version 9.1: statistics. SAS Institute, Cary, NC.
- Schlumbohm, C. & Harmeyer, J. (2006). Pregnancy impairs ketone body disposal in late gestating ewes: Implications for onset of pregnancy toxaemia. Research in veterinary Science, 81, 254-264.
- Schlumbohm, C. & Harmayer, J. (2003). Hypocalcemia reduces endogenous glucose production in Hyperketonemic sheep. Journal of Dairy Science, 86, 1953-1962.
- Schlumbohm, C., Sporleder, H. P., Gurtler, H. & Harmeyer, J. (1997). The influence of insulin on metabolism of glucose, free fatty acids and glycerol in normo- and hypocalcaemic ewes during different reproductive stages. Deutsche Tierarztliche Wochenschrift, 104, 359-365.
- Schoenberg, K.M., Ehrhardt, R.M. & Overton, T.R. (2012). Effects of plane of nutrition and feed deprivation on insulin responses in dairy cattle during late gestation. Journal of Dairy Science, 95, 670-682.
- Schwab, C. G. Boucher, S. E. & Sloan, B. K. (2007). Metabolizable protein and amino acid nutrition of the cow. Proceedings of the Cornell Nutrition Conference for Feed Manufacturers, Pages 121-138.
- Schwab, C. G., Ordway, R. S. & Whitehouse, N. L. (2004). Amino acid balancing in the context of MP and RUP requirements. 15th Annual Florida ruminant nutrition symposium, pp. 10-25.
- Schwab, C. G. & Ordway, R. S. (2003). Methionine supplementation options. Department of animal and nutritional science. University of New Hampshire.
- Schwab, C. G., Bozak, C. K., Whitehouse, N. L. & Messbah, M. M. A. (1992). Amino acid limitation and Qow to duodenum at four stages of lactation. 1. Sequences of lysine and methionine limitation. Journal of Dairy Science, 75, 3486-3502.
- Sejrsen, K., Hvelplund, T. & Nielson, M. O. (2006). Ruminant physiology.Wageningen Academic Publishing.
- Socha, M. T., Schwab, C. G., Putnam, D. E., Whitehouse, N. L., Garthwaite, B. D. & Ducharme, G. A. (2008). Extent of methionine limitation in peak-, early-, and mid-lactation dairy cows. Journal of Dairy Science, 91, 1996-2010.
- Staples, L. D., Mcphee, S. R., Williams, A. H. & Johnson, R. J. (1992). Rumen protected DL-methionine stimulates wool and body growth in grain supplemented Merino ewe lambs on summer pasture. Proc. Nutr. Soc. Aust 17.
- Tietze, M., Gruszecki, T., Lipecka, C., Szymanowska, A., Markiewicz, J. & Bryl, M. (2001). Level of selected biochemical indices in blood serum and health state of mainmary glands in sheep under different environment Systems. Arch. Tierz., Dummerstorf 44, Special Issue, 219-223.
- Van Emon, M.L., Schauer, C. S., Lekatz, L. A., Eckerman, S. R., Maddock, C. K. & Vonnahme, K. A. (2014). Supplementing metabolizable protein to ewes during late gestation: I. Effects on ewe performance and offspring performance from birth to weaning. Journal of Animal Science, 92, 339-348.
- Weekes, T. L., Luimes, P. H. & Cant J. P. (2006). Responses to amino acid imbalances and deficiencies in lactating dairy cows. Journal of Dairy Science, 89, 2177-2187.
- Yang, C. M. J., Schingoethe, D. J. & Casper, D. P. (1986). Protected Methionine and Heat-Treated Soybean Meal for High Producing Dairy Cows. Journal of Dairy Science, 69, 2348-2357.