Iranian Journal of animal Science

Iranian Journal of animal Science

Effects of different levels of rice bran with and without multi-Enzyme supplementation on performance, egg quality and serum biochemical parameters in commercial ̎Hy-line W-36̎ laying hens

Document Type : Research Paper

Authors
Alireza Safamehr 1
Hadi Attarhoseini 2
1 Professor, Department of Animal Sciences, Maragheh Branch, Islamic Azad University, Maragheh, East Azerbaijan, Iran
2 Former Graduate Student, Department of Animal Science Islamic Azad University, Maragheh Branch
10.22059/ijas.2016.61664
Abstract
The present study was conducted to determine the effects of rice bran and dietary multi-enzyme (Natuzyme) on performance, egg quality, and blood biochemical parameters of 288 laying hens during 12 week. This experiment was conducted in a completely randomized design in a factorial arrangement of 4×2, with four different levels of rice bran (0, 5, 10 and 15%) and two levels of multi enzyme (0 and 0.05%) with three replicates (n=12 birds). Egg production, egg weight, feed intake, feed conversion ratio and egg mass were not influenced by rice bran and enzyme supplementation. The effects of different levels of rice bran and supplementation of enzyme were not significant on Haugh unit, specific gravity, eggshell weight, eggshell thickness and shell weight/surface area. Also the effects of different levels of rice bran and supplementation of enzyme were not significant on albumin, blood total protein, uric acid, globulin, cholesterol, triglycerid and glucose. It was concluded that rice bran could be used up to 15% in laying hens diets without any detrimental effect on performance and egg quality.
Keywords
Biochemical
egg quality
laying hens
multi-enzyme
rice bran
  1. Aimonen, EM. & Uusi-Rauva, E. (1991). Replacement of barley by oats and enzyme supplementation in diets for laying hens. 2. Interior quality and chemical composition of eggs. Poultry Science, 4, 193-205.
  2. Annison, G., Moughan, PJ. & Thomas D.V. (1995). Nutritive activity of soluble rice bran arabinoxylans in broiler diets.  British Poultry Science, 36, 479-488.
  3. AOAC. (2002). Official methods of analysis of the association of official analytical. Eds. Washington. DC. pp:125-193.
  4. Bedford, MR. & Schulze, H. (1998). Exogenous enzymes for pigs and poultry. Nutrition Research Review, 11, 91-114.
  5. Berg, L.R. (1959) Enzyme supplementation of barley diets for laying hens. Poultry Science, 38, 1132-1139.
  6. Brenes, A., Guenter, W., Marquardt, R.R. & Rotter, B.A. (1993). Effect of ß-glucanase/pentosanase supplementation on the performance of chickens and laying hens fed wheat, barley, naked oats and rye diets. Canadian Journal Animal Science, 73, 941-951.
  7. Carlos. A.B. & Edwards Jr. (1998). The effects of 1.25-dihydroxy cholecalciferol and phytase on the natural phytate phosphorus utilization by laying hens. Poultry Science, 77, 850-858.
  8. Chowdhury, S.R. & Smith T.K. (2002). Dietary interaction of 1, 4-diaminobutane (putrescine) and calcium on eggshell quality and performance in laying hens. Poultry Science, 81, 84-91.
  9. Ersin-samli, H.N., Senkoylu, H., Akyurek & Agma, A. (2006). Using rice bran in laying hens diets. Cent Eurp Agic, 1, 135-140.
  10. Farkhoy, M., Sigharody, F. & Niknafas, F. (1994). Poultry nutrition. Second Edition. Coasar Publication. pp: 150-266.
  11. Farrell, D.J. & Martin, E.A. (1998a). Strategies  to improve the  nutritive  value  of  rice  bran  in  poultry  diets. I. The addition of food enzymes to target the non-starch polysaccharide fractions in diets of chickens and ducks give no response. British Poultry Science, 39, 549-554.
  12. Farrell, D.J. & Martin, E.A. (1998b). Strategies to improve the nutritive value of rice bran in poultry diets. III. The addition of inorganic phosphorus and a phytase to duck diet. British Poultry Science, 39, 601-611.
    1. Folch, J., Less, M. & Solane Stanley, G.H. (1956). A simple method for the isolation and purification of total lipid from animal tissues. Journal of Biological Chemistry, 226, 497-509.
    2. Gollinger, C.I., Suarez, D.M. & Irazusta, A (2004). Effects of rice bran inclusion on performance and bone mineralization in broiler chicks. Journal Applied  Poultry Research, 13, 183-190.
    3. Gordon, R.W. & Roland, D.A. (1998). Influence of supplemental phytase on calcium and phosphorus utilization in laying hens. Poultry Science 77, 290-294.
    4. Grobas, S, Me´ndez, J. La´zaro de, R.  Blas, C & Mateos, G.G. (2001). Influence of source and percentage of fat added to diet on performance and fatty acid composition of egg yolks of two strains of laying hens. Poultry Science, 80, 1171–1179.
    5. Hy-line International Publication. (2006). Hy-Line variety W-36 commercial management guide. 2006-2008. West Des Moines, Iowa. U.S.A.
    6. Iji, P.A. (1999). The impact of cereal non-starch polysaccharides on intestinal development and function in broiler chickens.  World's British Poultry Science Journal, 55, 375-387.
    7. Jalal, M.A. & Scheideler, S.E. (2001). Effect of supplementation of two different sources of phytase on egg production parameters in laying hens and nutrient digestibility. Poultry Science, 80, 1463-1471.
    8. Jin, L.Z.Ho-Y., Abdullah, T.W. & jalaludin, S. (1997). Probiotic in poultry modes of action. Poultry Science Journal, 53, 351-368.
    9. Kang, C.W. (1997). Nutritional values of rice bran and effects of its dietary Supplementation on the performance of broiler chickens. Konkuk University. Seoul, Korea.
    10. Lesson S, Summers JD. (1991). Commercial poultry nutrition. University Books Guelph Ontario Canada.
    11. Malathi, V. & Devegowda, G. (2001). In vitro evaluation of nonstarch polysaccharide digestibility of feed ingredients by enzymes. Poultry Science, 80, 302–305.
    12. Martin, E.A., Nolan, J.V., Nitsan, J. & Farrell, D.J (1998). Strategies to improve the nutritive value of rice bran in poultry diets. IV. Effects of addition of fishmeal and a microbial phytase to duckling diets on bird performance and amino acid digestibility.  British Poultry Science, 39, 612-621.
    13. McNaughtun, J.L. (1978). Effect of dietary fiber on egg yolk, liver, and plasma cholesterol concentrations of the laying hen. Journal Nutrition, 108, 1842-1848.
    14. Mogaddam Tabrizi, M. & Salarmoieni, M. (2011). The Effect of Using Different Levels of Rice Bran either with or without Multi-Enzyme Supplementation on Broiler Chicks' Performance. Iranian Journal Animal Science, 42(1), 29-48.
    15. Nahashon, S.N., Nakaue, H.S. & Mirosh, I.W. (1996). Performance of single comb white leghorn fed a diet supplemented with a live microbial during the growth and egg laying phases. Animal Feed Science and Technology, 57, 25-38.
    16. National Research Council. (1994). Nutrient Requirements of Poultry. 9th ed., Washington DC, National Academy Press. pp: 44-45.
    17. Nazifi S. (1997). Hematology and Clinical Biochemistry of Birds. Shiraz University Publication.
    18. Nelson, T.S., Shieh, T.R., Wodsinski, R.J. & Ware, J.H. (1968). The availability of phytate phosphorus in soybean meal before and after treatment with a mold phytase. British Poultry Science, 47, 1842-1848.
    19. Nobakht, A. (2007). The effects of Inclusion different levels of rice bran in laying hens diets on performance and plasma and Egg yolk cholesterol contents. International Poultry Science, 6(9), 1120-1124.
    20. Ravindran, V. Bryden, W.L. & Kornegay, E.T. (1995). Phytase: Occurrence, bioavailability and implications in poultry nutrition. Poultry Avain Biology Review, 6, 125-143.
    21. SAS Institute.  (1998) SAS User’s Guide: Statistics. SAS institute Inc., Cary, NC.
    22. Scheideler, S.E., Beck, M.M., Abudabos, A. & Wyatt, C.L. (2005). Multiple-enzyme (Avizyme) supplementation of corn-soy based layer diets. Journal Applied Poultry Research, 14, 77-86.
    23. Schneitz, C., Kiiskinen, T., Toivonen, V. & Nási, M. (1998). Effect of Broilact on the physicochemical conditions and nutrient digestibility in the gastrointestinal tract of broilers. Poultry Science, 77, 426-432.
    24. Scott, T.A., Kampen, R. & Silversides, F.G. (1999). The effect of  phosphorus,  phytase enzyme,  and  calcium  on  the  performance  of  layers  fed  corn-based  diets.  Poultry Science, 78, 1742-1749.
    25. Simons, P.C.M., Jongbloed, A.W., Versteegh, H.A.J. & Kemme, P.A. (1992). Improvement of phosphorus availability by microbial phytase in poultry and pigs. P. 100-108 in: Proceedings of the Georgia Nutrition Conference for the Feed Industry, Atlanta, GA.
    26. Smits, C.H.M. & Annison, G. (1996). Non-starch plant polysaccharides in broiler nutrition towards a physiologically valid approach to their determination. World's Poultry Science, J 52, 203-221.
    27. Sohail, S.S. & Roland, D.A (1999). Influence of supplemental phytase on performance of broilers four to six weeks of age.Poultry Science, 78, 550-555.
    28. Stanley, V.G., Sefton, A.E. & Chukwe, H. (1995). The interaction of temperature, mannoligocharides and aflatoxin on broiler chicks. 10th World’s Poultry Science Association Conference on Poultry Nutrition. Antalya, Turkey. Pages 2-7 in Proc.
    29. Stanley, V.G., Sefton, A.E. & Chukwe, H. (1995). The interaction of temperature, mannoligocharides and aflatoxin on broiler chicks. 10th World’s Poultry Science Association Conference on Poultry Nutrition. Antalya, Turkey. Pages 2-7 in Proc.
    30. Sturkie, P. D. (1995). Avian physiologhy. 4th ed. Springer Verlag. New York, pp: 115 -270.
    31. Tangendjaja, B., Chung, T.K. & Broz, J. (2002). Effects of different sources of microbial phytase on production performance of Brown-Egg layers fed diets containing a High level of Rice bran. Applied World’s Poultry Science Association Conference on Poultry Nutrition, 11, 212-216.
    32. Van der Klis, J.D., Versteegh, H.A.J., Simons, P.C.M. & Kies, A.K. (1997). The efficacy of phytase in corn-soybean meal based diets for laying hens. Poultry Science, 76, 1535-1542.
    33. Warren, B.E. & Farrell, D.J. (1990). The nutritive value of full fat and defatted Australian rice bran. II. Growth studies with chickens, rats and pigs. Animal Feed Science Technology, 27, 229-246.
    34. Yalçin, S., Oğuz, F., Güçlü, B. & Yalçin, S. (2009). Effects of dietary dried baker's yeast on the performance, egg traits and blood parameters in laying quails. Tropical Animal Health Production, 41(1), 5-10
    35. Yurong, Y., Ruiping, S., Shimin, Z. & Yibao, J. (2005). Effect of probiotics on intestinal mucosal immunity and ultrastructure of cecal tonsils of chickens. Arch Animal Nutrition, 59, 237-46.

  • Receive Date 23 February 2015
  • Revise Date 13 December 2015
  • Accept Date 13 December 2015