Investigation of in vitro digestibility, fermentation and enzyme activity of rumen anaerobic fungi of dromedary camels fed by cultivated and pasture forages

Document Type : Research Paper


1 Ph.D Student of Animal Nutrition, Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khouzestan, Khouzestan, Iran

2 Assistant Professors, Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khouzestan, Khouzestan, Iran

3 Assistant Professor, Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khouzestan, Khouzestan, Iran

4 Associate Professor, Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khouzestan, Khouzestan, Iran


The aim of this study was to investigate the fermentation characteristics and cellulolytic enzymes activities of dromedary camels rumen anaerobic fungi in fed with cultivated and pasture forages. Hence, the inoculant of rumen anaerobic fungi was prepared by using specific medium and the gas production, digestibility and enzyme activity of rumen fungi were determined on the basis of a 2 × 2 factorial design. The results showed that gas production potential was higher in treatments containing atriplex substrate, but the rate of gas production was higher in wheat straw treatments. Also the total gas production (50.71 ml) in treatment with rumen fluid of camel fed pasture forage and atriplex substrate was the highest (P≤0.01). The medium's ammonia nitrogen at different times of incubation was affected by the type of feed and substrate (P≤0.01). The results showed that the organic matter digestibility was not affected by the type of feed but was decreased in atriplex treatments by changing substrate. Also digestibility of DM and NDF in atriplex treatment showed increasing trend at the end of experiment. Endo and exoglucanas activity of rumen fungus in treatment with rumen fluid of camel fed with pasture forage and atriplex substrate had the highest increase (P≤0.01) in the final hours of incubation. It seems that for in vitro evaluating fermentation of halophilic forages, it is better to use a rumen fluid accordance with substrate.


  1. Abu-Zanat, M. M. W. & Tabbaa, M. J. (2006). Effect of feeding Atriplex browse to lactating ewes on milk yield and growth rate of their lambs. Small Ruminant Research, 64 (1-2), 152-161.
  2. Akin, D. E. & Benner, R. (1988). Degradation of polysaccharides and lignin by ruminal bacteria and fungi. Applied Environmental Microbiology, 54, 1117-1125.
  3. Angaji, L., Souri, M. & Moeini, M. M. (2011). Deactivation of tannins in raisin stalk by polyethylene glycol-600: Effect on degradation and gas production in vitro. African Journal of Biotechnology, 10(21), 4478-4483.AOAC (2000). Official methods of analysis. )17th Edit(. Association of Official Analytical Chemists (Gaithersburg, MD, USA).
  4. Blummel, M., Makkar, H. P. S. & Becker, K. (1997). In vitro gas production: a technique revisited. Journal of Animal Physiology and Animal Nutrition, 77, 24-34.
  5. Bernalier, A., Fonty, G., Bonnemoy, F. & Gouet, P. (1992). Degradation and fermentation of cellulose by the rumen anaerobic fungi in axenic cultures or in association with cellulolytic bacteria. Current Microbiology, 25, 143-148.
  6. Broderick, G. A. & Kang, J. H. (1980). Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. Journal of Dairy Science, 63, 64-75.
  7. Chen, Y. C., Hseu, R. S. & Cheng, K. J. (2003). The genetic similarity of different generations of Neocallimastix frontalis SK. FEMS Microbiology Letters, 221, 227-231.
  8. Colombatto, D. & Beauchemin, K.A. (2003). A proposed methodology to standardize the determination of enzymic activities present in enzyme additives used in ruminant diets. Canadian Journal of Animal Science, 83, 559-568.
  9. Cone, J. W., Van Gelder, A. H., Visscher, G. J. W. & Oudshoorn, L. (1996). Influence of rumen fluid and substrate concentration on fermentation kinetics measured with a fully automated time related gas production apparatus. Animal Feed Science and Technology, 61, 113-128.
  10. Dashtban, M., Schraft, H. & Qin, W. (2009). Fungal bioconversion of lignocellulosic residues; opportunities & perspectives. International Journal Biological Science, 5, 578-595.
  11. Dereje, M. & Uden, P. (2005). The browsing dromedary camel: I. Behaviour, plant preference and quality of forage selected. Animal Feed Science and Technology, 121, 297-308.
  12. Dulphy, J. P., Dardillat, C., Jailler, M. & Ballet, J. M. (1997). Comparative study of forestomach digestion in llamas and sheep. Reproduction and Nutrition Development, 37, 709-725.
  13. El Shaer, H. M. (2010). Halophytes and salt-tolerant plants as potential forage for ruminants in the Near East region. A Review. Small Ruminant Research, 91, 3-12.
  14. Haddi, M. L., Arab, H., Yacoub, F., Hornick, J. L., Rollin, F. & Mehennaoui, S. (2009). Seasonal changes in chemical composition and in vitro gas production of six plants from Eastern Algerian arid regions. Livestock Research for Rural Development, 21(4).
  15. Hassan, A. A. (2009). Effect of some Enrichment and Nawaz biological treatments on a melioration utilization of Atriplex nummularia fed by sheep. Egyptian Journal of Nutrition and Feeds, 12 (3) Special Issue, 553-566.
  16. Hassan Sallam, S. M. A., Da Silva Bueno, I. C., De Godoy, P. B., Eduardo, F. N., Schmidt Vittib, D. M. S. & Abdalla, A. L. (2010). Ruminal fermentation and tannins bioactivity of some browses using a semi-automated gas production technique. Tropical and Subtropical Agroecosystems, 12, 1-10.
  17. Heckathorn, S. A., McNaughton, S. J. & Cleman, J. S. (1999). In: C4 Plant, Biology, Sage, R.F., Monson, R.K. (Eds.), C4 Plants and herbivory. Academic Press, San Diego, CA, pp. 285-312.
  18. Jones, G. A., McAllister, T. A., Muir, A. D. & Cheng, K. J. (1994). Effects of sainfoin (Onobrychis viciifolia Scop.) condensed tannins on growth and proteolysis by four strains of ruminal bacteria. Applied Environmental Microbiology, 60, 1374-1378.
  19. Kassily, F. N. (2002). Forge quality and camel feeding patterns in central baringo. Kenyaian Livestock Production Science, 78 (2), 175 - 182.
  20. Lee, S. S., Ha, J. K. & Cheng, K. J. (2000). Relative Contributions of Bacteria, Protozoa, and Fungi to in vitro degradation of orchard grass cell walls and their interactions. Applied Environmental Microbiology, 3807-3813.
  21. Lemosquet, S., Dardillat, C., Jailler, M. & Dulphy, J.P. (1996). Voluntary intake and gastric digestion of two hays by llamas and sheep: influence of concentrate supplementation. Journal of Agricultural Science Camb, 127, 539-548.
  22. Makkar, H. P. S. & McSweeney, C. S. (2005). Methods in Gut Microbial Ecology for Ruminants. International Atomic Energy Agency. Published by Springer. P 25.
  23. Min, B. R. Attwood, G. T., McNabbb, W. C., Molanb, A. L. & Barry, T. N. (2005). The effect of condensed tannins from Lotus corniculatus on the proteolytic activities and growth of rumen bacteria. Animal Feed Science and Technology, 121, 45-58.
  24. Minson, D. J. (1971). Influences of lignin and silicon on a summative system for assessing the organic matter digestibility of Panicum. Australian Journal of Agricultural Research, 22, 589-598.
  25. Mohammadabadi, T., Chaji, M. & Bojarpour, M. (2012). The effect of steam pressure treatment on gas production parameters of sugarcane pith using isolated Rumen microbiota. Journal of Animal Science Researches, 4(3), 240-246. (in Farsi)
  26. Nagpal, R., Puniya, A. K. & Singh, K. (2009). In vitro fibrolytic activities of the anaerobic fungus. Caecomyces sp., immobilized in alginate beads. Journal of Animal Feed Science, 18, 758-768.
  27. Orpin, C. G. (1975). Studies on the rumen flagellate Neocallimastix frontalis. General Microbiology, 91, 249-262.
  28. Orskov, E. R. & McDonald, I. (1979). The estimation of protein degradability in the rumen from incubation measurement weighted according to rat of passage. Journal of Agricultural Science, 92, 499-503.
  29. Paul, S. S., Kamra, D. N., Sastry, V. R. B. & Agarwal, N. (2004). Effect of administration of an anaerobic gut fungus isolated from wild blue bull to buffaloes on in vivo ruminal fermentation and digestion of nutrients. Animal Feed Science and Technology, 115,143-157.
  30. Russell, J. B. & Strobel, H. J. (1989). Effect of ionophores on ruminal fermentation. Applied Environmental Microbiology, 55, 1-6.
  31. Sage, R. F., Sage, T. L. & Kocacinar, F. (2012). Photorespiration and the Evolution of C4 Photosynthesis. Annual Review of Plant Biology, 63, 19-47.
  32. SAS (2005). User’s Guide. Release 6.08. SAS Institute Inc., Cary, NC.
  33. Schofield, P., Pitt, R. E. & Pell, A. N. (1994). Kinetics of fiber digestion from in vitro gas production. Journal of Animal Science, 72, 2980-2991.
  34. Schillhorn, V. V. & Leoffler, T. W. (1990). Mineral deficiencies in ruminants in Sub-Saharan Africa: a review. Tropical Animal Health and Production, 22, 197-205.
  35. Sharifi, M. & Khadem, A. A. (2012). Dynamic fermentation in ruminants animal products to biogas. Adine book. 281-282.
  36. Shawket, M. S. & Ahmed, M. H. (2009). Effect of prolonged feeding Atriplex (saltbush) to camels on digestibility, nutritive value and nitrogen utilization. Egyptian Journal of Nutrition and Feeds, 12 (3) Special Issue: 205- 214.
  37. Shawket, S. M., Youssef, K. M. & Ahmed, M. H. (2010). Comparative evaluation of Egyptian clover and Atriplex Haliums diets for growth and milk production in camel (Camelus dromedarius). Animal Science Reporter, 4, 9-20.
  38. Sirohi, S. K., Choudhury, P. K., Dagar, S. S., Puniya, A. K. & Singh, D. (2012). Isolation, characterization and fibre degradation potential of anaerobic rumen fungi from cattle. Annuals of Microbiology, DOI 10.1007/s13213-012-0577-6.
  39. Sliwinski, B. J., Soliva, C. R., Machmuller, A. & Kreuzer, M. (2002). Efficacy of plant extracts rich in secondary constituents to modify rumen fermentation. Animal Feed Science and Technology, 101, 101-114.
  40. Smith, G. S. (1992). Toxification and detoxification of plant compounds by ruminants: an overview. Journal of Range Management, 45, 25-30.
  41. Smith, A. H., Zoetendal, E. & Mackie, R. I. (2005). Bacterial mechanisms to overcome inhibitory effects of dietary tannins. Microbiology Ecology, 50, 197-205.
  42. Soltan, Y. A., Morsy, A. S., Sallam, S. M. A., Louvandini, H. & Abdalla, A. L. (2012). Comparative in vitro evaluation of forage legumes (prosopis, acacia, atriplex, and leucaena) on ruminal fermentation and methanogenesis. Animal and Feed Science, 21, 759-772.
  43. Tavendale, M. H., Meagher, L. P.,  Pacheco, D., Walker, N., Attwood, G. T. & Sivakumaran, S. (2005). Methane production from in vitro rumen incubations with Lotus pedunculatus and Medicago sativa, and effects of extractable condensed tannin fractions on methanogenesis. Animal Feed Science and Technology, 123(1), 403-419.
  44. Teferedegne, B. (2000). New perspectives on the use of tropical plants to improve ruminant nutrition. Proceedings of the Nutrition Society, 59, 209-214.
  45. Thareja, A., Puniya, A. K., Goel, G., Nagpal, R., Sehgal, J. P., Singh, P. & Singh, K. (2006). In vitro degradation of wheat straw by anaerobic fungi from small ruminants. Archive of Animal Nutrition, 60, 412-417.
  46. Theodorou, M. K., Williams, B. A., Dhanoa, M. S., McAllan, A. B. & France, J. (1994). A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology, 74, 3583-3597.
  47. Towhidi, A., Saberifar, T. & Dirandeh, E. (2011). Nutritive value of some herbage for dromedary camels in the central arid zone of Iran. Tropical Animal Health and Production, 43, 617-622.
  48. Van Soest, P. J., Robertson, J. B. & Lewis, B. A. (1991) Methods of dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74, 3583- 3597.
  49. Von-Engelhardt, W., Dycker, C. H. & Lechner-Doll, M. (2007). Absorption of short-chain fatty acids, sodium and waterfrom the forestomach of camels. Journal of Comparative Physiology, 177, 631-640.
  50. Waghorn, G. C., Shelton, I. D., McNabb, W. C. & McCutcheon, S. N. (1994). Effects of condensed tannin in Lotus pedunculatus on nutritive value for sheep. 2. Nitrogenous aspects. Journal of Agricultural Science, 123, 109-119.
  51. Wang, Y., McAllister, T. A., Yanke, L. J., Xu, Z. J., Cheeke, P. R. & Cheng, K. J. (2000). In Vitro effects of steroidal saponins from Yucca sc hidigera extract on rumen microbes. Journal of Applied Microbiology, ‌88, 887-896.
  52. Wilson, R.T. (1998). Camels. Macmillan Education Ltd., London.
  53. Zhang, Y., Gao, W. & Meng, Q. (2007). Fermentation of plant cell walls by ruminal bacteria, protozoa and fungi and their interaction with fibre particle size. Archives of Animal nutrition, 61(2), 114-125.