Identification of new miRNAs and isomirs in liver tissue of dairy cows

Document Type : Research Paper


1 M. Sc. Student, Department of Animal and Poultry Sciences, Aburaihan Campus, University of Tehran, Iran

2 Assistant Professor, Department of Animal and Poultry Sciences, Aburaihan Campus, University of Tehran, Iran

3 Associate Professor, Department of Animal and Poultry Sciences, Aburaihan Campus, University of Tehran, Iran


Negative energy balance (NEB) occurs inhigh-producing dairy cows in first few weeks after parturition, that energy demand for maintenance and milk production exceeds the dietary energy intake. NEB has a considerable economic importance due to negative effect on health and fertility in dairy herds, therefore, the identification of its effective regulatory mechanism is important. miRNAs are one of these effective regulatory factors in NEB. Despite of the importance of NEB, the regulatory mechanisms related to miRNAs has not been well documented. In this study miRNA-seq data from liver tissue of eight Holstein dairy cows were analyzed to identify new miRNAs and isomirs. All data have been achieved from GEO in NCBI database. A total of 291 new miRNAs with homologous gene in other species were identified. Moreover, 164 new miRNAs without homologous were identified. Investigation of target genes of these miRNAs lead to identify biological paths related to NEB. Also 466 new isomiR and 95 new miRNA* were detected for the first time in cow genome. The results of the current study provide new information for better understanding of the regulatory roles of miRNAs in NEB.


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