Iranian Journal of animal Science

Iranian Journal of animal Science

Effect of different dietary enzyme supplemented non-starch polysaccharides on growth performance, villus morphology, enzyme activity and gene expression of nutrient transporters and mucin producer in the small intestine of broiler chickens

Document Type : Research Paper

Authors
Akbar Yaghobfar 1
Majid Kalantar 2
Faroborz Khajeali 3
1 Professor, Animal Science Research Institute, Agricultural Research, Education, and Extension Organization, Tehran, Iran
2 Assistant Professor, Animal Science Department of Agricultural Research Center of Qom, Agricultural Research, Education, and Extension Organization. Tehran, Iran, and former PhD Student of Shahrekord University, Shahrekord, Iran
3 Professor, Animal Science Department of Shahrekord University, Shahrekord, Iran
10.22059/ijas.2016.59798
Abstract
 

This experiment was conducted to evaluate the effect of different dietary enzyme supplemented non-starch polysaccharides on growth and physiological traits of broilers. 625 one day old Ross-308 broiler chickens were allocated randomly to 5 treatments with 5 replicates using a CRD statistical design through 1 to 21 and 22 to 42 days of rearing period. Treatments were included control, wheat, wheat + enzyme, barley and barley + enzyme. According to the results, effect of various diets with different source of NSP on growth traits was significant and wheat or barley diets supplemented with enzymes had higher means than those without enzymes (p<0.01). Effect of various diets with different source of NSP supplemented with or without enzymes on villus morphology was significant (p<0.01). Wheat and barley without enzymes resulted in reducing villus height, adversely increasing villus width and crypt depth in three parts of intestine, but supplementation with enzymes restored the adverse effects (p<0.01). Wheat and barley without enzymes resulted in higher pancreatic enzyme activity and serum enzyme levels (p<0.01). Also these two diets resulted in higher gene expression for nutrient transporters and mucin producer in the intestine but supplementation with enzymes restored the situation (p<0.01). As a final result using of different source of non-starch polysaccharides without enzymes in this experiment resulted in destruction of broiler growth and physiological traits but supplementation with enzymes restored the undesirable effects.
Keywords
Broilers
Enzyme activity
gene expression
non-starch carbohydrate
villus morphology
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Iranian Journal of animal Science
Volume 47, Issue 3 - Serial Number 3
Autumn 2016
Pages 429-440

  • Receive Date 13 January 2015
  • Revise Date 08 July 2016
  • Accept Date 13 July 2016