Iranian Journal of animal Science

Iranian Journal of animal Science

Identification of novel SNP in promoter of Insulin-Like Growth Factor-I (IGF1) gene in Japanese quail by PCR-SSCP assay

Document Type : Research Paper

Authors
Homa Aarabi 1
Mohammad Moradi Shahrbabak 2
Abbas Pakdel 3
Hossein Moradi Shahrbabak 4
Ali Esmailizadeh koshkoiyeh 5
1 Former Ph.D. Student, Department of Animal Science, Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Professor, Department of Animal Science, Agriculture & Natural Resources, University of Tehran, Karaj, Iran
3 Associate Professor, Faculty of Agriculture, University of Isfahan, Iran
4 Assistant Professor, Department of Animal Science, Agriculture & Natural Resources, University of Tehran, Karaj, Iran
5 Professor, Faculty of agriculture, Shahid Bahonar University of Kerman, Iran
10.22059/ijas.2016.59035
Abstract
Insulin-like growth factor-1 gene (IGF1) is a biological candidate gene for investigation traits such as growth, body weight and muscles growth in different species. In this research eight pairs of white and wild quails were crossed reciprocally as a base population. A total of 34 quails were produced in first generation (F1) and 422 quails were generated by intercrossing the F1 population. Body weights at the time of hatching and different weeks were recorded in the second generation. The association between IGF1 promoter region polymorphism and body weight in different ages was investigated in F2 generation. Using PCR-SSCP assay and sequencing, a novel single nucleotide polymorphism (SNP) was identified in IGF1 promoter region in 472 birds from three Generations. Genotypic frequencies of AA, AG and GG genotypes were 0.05, 0.62 and 0.33, respectively for all generations. The frequency of A and G alleles were 0.36 and 0.64, respectively. The AA genotype was lower than AG and GG genotypes for body weights in ages of one to five weeks and slaughter time and the average of daily gain was also lower from week first to four but these differences weren’t significant. The average of daily gain was higher in females compared to males for AG genotype (P<0.001). Comparison between detected allele in the present study with reported allele by other research groups in chickens revealed a novel SNP in promoter of IGF1 gene in Japanese quail, but association between this polymorphism with body weight and growth rate were not significant.
Keywords
growth traits
Insulin-Like Growth Factor-I
Japanese quail
PCR-SSCP assay
sequencing
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Iranian Journal of animal Science
Volume 47, Issue 2 - Serial Number 2
Autumn 2016
Pages 303-312

  • Receive Date 30 September 2015
  • Revise Date 02 March 2016
  • Accept Date 09 March 2016