Document Type : Research Paper


1 Ph.D. Student, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran

2 Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran

3 Assistant Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran and Assistant Professor of Razi Vaccine and Serum Research Institute, Karaj, Iran

4 Assistant Professor, Razi Vaccine and Serum Research Institute, Karaj, Iran

5 Medical Laboratory Expert, Razi Vaccine and Serum Research Institute, Karaj, Iran

6 Veterinary Expert, Razi Vaccine and Serum Research Institute, Karaj, Iran


Four mature stallions were used to study the effect of enzymatic antioxidant catalase on freezability of Turkmen stallion's sperm. Collected semen from stallions were processed and pooled before being divided treatments (200×106  sperm/ml) and diluted with extenders supplemented with different levels of catalase [0 (control), 100 U/ml (CAT100), 150U/ml (CAT150) and 200U/ml (CAT200)]. Extended and supplemented semen's were frozen according to a standard protocol. After thawing, motility, viability, membrane integrity, total abnormality and lipid peroxidation were assessed. Results showed that addition of 200U/ml catalase could improve progressive and total motility, viability, membrane integrity and decreased lipid peroxidation compared to control and other levels of catalase (p<0.05). In addition, 150 U/ml catalase improved total motility, viability and decreased MDA concentration (p<0.05). However, the addition of 100 U/ml catalase had no significant effect on sperm quality (p>0.05). The results showed that different levels of catalase could not decreased total abnormality (p>0.05). We concluded that addition of 200U/ml catalase could improve freezability of Turkmen stallion’s sperm.


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