Iranian Journal of animal Science

Iranian Journal of animal Science

Effects of alfalfa maturity and cutting times on availability of alfalfa hay protein for dairy cows

Document Type : Research Paper

Authors
Safora Yousefinezhad 1
Farshid Fattahnia 2
Mehdi Rashnavadi 3
Sayed Gholamreza Mossavi 1
Golnaz Taasoli 3
1 Former M.Sc. Student, Department of Animal Science, University of Ilam, Ilam, Iran
2 Associate Professor, Department of Animal Science, University of Ilam, Ilam, Iran
3 Assistant Professor, Department of Animal Science, University of Ilam, Ilam, Iran
10.22059/ijas.2016.59023
Abstract
This experiment was conducted to consider the effects of maturity and cutting time on chemical composition and available protein content of alfalfa hay for dairy cows.. Alfalfa hay harvested at two maturity stages (Pre-bloom and Early bloom) and two cutting times (Morning and Afternoon). Available protein was predicted using NRC (2001) and DVE/OEB (1994) models. In the NRC model (2001), alfalfa hay harvested pre-bloom and in the afternoon had higher microbial protein synthesized in the rumen from energy and absorbable microbial protein and lower ruminal degraded protein balance (P<0.05). But, metabolizable protein content was not affected by maturity stage and cutting time (P>0.05). In the DVE/OEB model, pre-bloom alfalfa harvested in the afternoon had higher fermentable organic matter, microbial protein synthesized from organic matter and absorbable microbial protein and lower ruminal degraded protein balance (P<0.05). In conclusion, pre-bloom alfalfa hay harvested in the afternoon had higher available protein for dairy cows.
Keywords
Alfalfa
Degradability
Microbial protein
protein evaluation
  1. Aliarabi, H. (1997). Evaluation of nutritional value of Vicia ervilia grain by in vitro and in vivo methods in Hamedan province. Msc Thesis. Tehran University. (in Farsi)
  2. AOAC. (2000). Official Methods of Analyses. Association of Official Analytical Chemists. Washington, D.C., USA.
  3. Alomar, D., Fuchslocher, R. & Stockebrand, S. (1999). Effects of oven- or freeze-drying on chemical composition and NIR spectra of pasture silage. Animal Feed Science and Technology, 80, 309-319.
  4. Balde, A. T., Vandersall, J. H., Erdman, R. A.,  Reeves, J. B. & Glenn, B. P. (1993). Effect  of  stage  of  maturity  of  alfalfa  and  orchard grass  on  in  situ  dry  matter  and  crude protein  degradability  and  amino  acid  composition.  Animal Feed Science and Technology, 44, 29-43.
  5. Bach, A., Calsamiglia, S. & Stern, M. D. (2005). Niterogen metabolism in the rumen. Journal of Dairy Science, 88 (E. Suppl.), E9-E21.
  6. Brito, F., Tremblay, G. F., Bertrand, A., Castonguay, Y., Belanger, G., Michaud, R., Lapierre, H., Benchaar, C., Petit, H. V., Ouellet, D. R. & Berthiaume, R. (2008). Alfalfa cut at sundown and harvested as baleage improves milk yield of late-lactation dairy cows. Journal of Dairy Science, 3968-3982.
  7. Brito, F., Tremblay, G. F., Bertrand, A., Castonguay, Y., Belanger, G., Michaud, R., Lapierre, H., Benchaar, C., Petit, H. V., Ouellet, D. R. & Berthiaume, R. (2009). Alfalfa  cut  at  sundown  and  harvested  as  baleage  increases  bacterial  protein  synthesis  in  late-lactation  dairy  cows.  Journal of. Dairy Science, 92, 1092-1107.
  8. Burns, J. C., Fisher, D. S.  & Mayland, H. F. (2007).  Diurnal  shifts  in  nutritive  value  of  alfalfa  harvested  as  hay  and  evaluated  by  animal  intake  and  digestion.  Crop Science, 47, 2190-2197.
  9. Chen, D., Peel, M. D., Olson, K. C., Weimer, B. C.  & DeWald, D. B. (2009). Differential ruminal degradation of alfalfa proteins. Canadian Journal of Plant Science, 89, 1065-1074.
  10. Edmunds, B., Sudekum, K. H., Spiekers, H. & Schwarz, F. J. (2012). Estimating  ruminal  crude  protein  degradation  of  forages  using  in  situ and  in  vitro  techniques. Animal Feed Science and Technology, 175, 95-105.
  11. Elizalde, J. C., Merchen, N. R. & Faulkner, D. B. (1999). Fractionation of fiber and crude protein in fresh forages during the spring growth. Journal of Animal Science, 77,476-484.
  12. Eshghi1, M.J., Mirzaei Aghjeh Qeshlagh, F., Seif Davati J. & Ghorbani. A. (2013). Determination of nutritional value and degradability of dry matter and cell wall of Agropyrontauri at different phenological stages in Neorregion (Ardabil province). Journal of Ruminant Researches, 1, 77-94. (in Farsi)
  13. Gomes, M. J., Hovell, F. D. D., Chen, X. B., Nengomasha, E. M., & Fikremariam, D. (1994). The effect of starch supplementation of straw on microbial protein supplies in sheep. Animal Feed Science and Technology, 49, 277-286.
  14. Hoffman, P.C., Sievent, S. J., Shaver, R. D., Welch, D. A. & Combs, D. K. (1993). In situ dry matter protein and fiber degradation of perennial forage. Journal of Dairy Science, 76, 2632-2642.
  15. Horadagoda, A., Fulkerson, W. J., Barchia, I., Dobos, R. C. & Nandra, K. S. (2008). The effect of grain species, processing and time of feeding on the efficiency of feed utilization and microbial protein synthesis in sheep. Livestock Science, 114, 117-126.
  16. Hoy, M. D., Kenneth, J. M., George, J. R., & Brummer, E. C. (2002). Alfalfa yield and quality as influenced by establishment method. Agronomy Journal, 94, 65-71.
  17. Jonker, A., Gruber, M. Y., Wang, Y., Coulman, B., Azarfar, A., McKinnon, J. J., Christensen, D. A. & Yu, P. (2011). Modeling degradation ratios and nutrient availability of anthocyanidin-accumulating Lc-alfalfa populations in dairy cows. Journal of Dairy Science, 94, 1430-1444.
  18. Karabulut. A., Canbulat, O. & Kamalak, A. (2006). Effect of maturity stage on the nutritive value of birdsfoot trefoil (Lotus corniculatus L.) hays. Lotus Newsletter, 36, 11-21.
  19. Karsli, M. A. & Russell, J. R. (2001). Effects of some dietary factors on ruminal microbial   protein synthesis. Turkish Journal of Veterinary Animal Science, 252, 681-686.
  20. Licitra, C., Hernandez, T. N. & Van Soest, P. J. (1996). Standardization of procedures for nitrogen fractionation of ruminant feeds Animal Feed  Science and  Technology, 57, 347-358
  21. Mehrdad, N., Alikhani, M. & Ghorbani, G.R. (2002). The effect of cuttings and growth stage on chemical composition and degradability of alfalfa. Agricultural and natural resource Science and technology Magazine, 8(2), 159-168. (in Farsi)
  22. NRC. (2001). Nutrient requirement of dairy cattle. 7th revised ed. National Academy press, Washington DC, USA.
  23. Nugent, J. H. A. & Mangan, J. L. (1981). Characteristics of the rumen proteolysis of fraction 1 (18S) leaf protein in Lucerne (Medicago sativa L.). British Journal of Nutrition, 46, 39-58.
  24. Orskov, E.R. & McDonald, I. (1979). The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of Agricultural Science, 92, 499-503.
  25. SAS. (2000). User’s Guide: Statistics. 8th edn. Statistical Analysis System Institute SAS/STAT, Cary, NC.
  26. Steacy, G. M., Christensen, D. A., Cochran, M. I. & Horton, G. M. J. (1983). An  evaluation  of  three  stages  of  maturity  of  hay  fed  with  two  concentrate  levels  for lactating  dairy  cows.  Canadian Journal of Animal Science, 63, 623-629.
  27. Tamminga,  S.,  Brandsma,  G.G.,  Dijksta,  J.,  Duinkerken,  G.V.,  Vuuren,  A.M.V. &  Blok, M.C. (2007).  Protein evaluation for ruminants:  the DVE/OEB 2007 system, CVB documentation report nr. 53.  Centraal Veevoeder Bureau, Lelystad, The Netherlands.
  28. Tamminga, S., Van Straalen, W. M., Subnel, A. P. J., Meijer, R. G. M., Steg, A., Wever, C. J. G. & Block, M. C. (1994).  The Dutch protein evaluation system: the DVE/OEB system. Livestock Production Science, 40, 139-155.
  29. Van Soest, P.J., Robertson, J.B. & Lewis, B.A.  (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74, 3583-3597.
  30. Van Soest, P. J. (1994). Nutritional Ecology of the Ruminant, 2nd ed. Cornell University Press, Ithaca, NY, USA.
  31. Webster, A.J.F., Kaya, S., Djouvinov, D.S., Kitcherside, M.A. & Glen, E.F. (2003). Purine excretion and estimated microbial protein yield in sheep fed diets differing in protein degradability. Animal Feed Science Technology, 105,123-134.
  32. Yari, M., Valizadeh, R., Naserian, A.A., Ghorbani, G.R., Rezvani Moghadam, P., Jonker, A. & Yu, P. (2012a). Botanical traits, protein and carbohydrate fractions, ruminal degradability and energy contents of alfalfa hay harvested at three stages of maturity and in the afternoon and morning. Animal Feed Science and Technology, 172, 162-170.
  33. Yari, M., Valizadeh, R., Naserian, A. A., Jonker, A. & Yu, P. (2012b). Modeling nutrient availability of alfalfa hay cut at three stages of maturity and in the afternoon and morning. Animal Feed Science and Technology, 178, 12-19.
  34. Yu, P., Christensen, D. A., Mckinnon, J. J. & Markert, J. D. (2003a). Effect of variety and maturity stage on chemical composition, carbohydrate and protein subfractions, in vitro rumen degradability and energy values of timothy and alfalfa. Canadian Journal of Animal Science, 83, 279-290.
  35. Yu, P., Christensen, D. A. & McKinnon, J. J. (2003b). Comparison of the national research council-2001 model with the Dutch system (DVE/OEB) in the prediction of nutrient supply to dairy cows from forages. Journal of Dairy Science, 86, 2178-2192.
Iranian Journal of animal Science
Volume 47, Issue 2 - Serial Number 2
Autumn 2016
Pages 185-191

  • Receive Date 11 January 2016
  • Revise Date 15 March 2016
  • Accept Date 11 April 2016