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چندشکلی ژن دی آسیل گلیسرول آسیل ترانسفراز 1 (DGAT1) در جمعیت گاومیش‌های ایران

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری گروه علوم دامی، دانشکده علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران

2 استاد، گروه علوم دامی، دانشکدۀ علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

3 دانشیار گروه علوم دامی، دانشکده علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران

4 استاد گروه بیوتکنولوژی حیوانی، دانشگاه هنک‌یونگ، انسئونگ، کرۀ جنوبی

چکیده

دی آسیل گلیسرول آسیل ترانسفراز1 (DGAT1) آنزیم کلیدی در ساخت (سنتز) تری گلیسیرید است. ایجاد یک جهش جایگزینی باعث تبدیل گوانین به آدنین و منجر به جایگزینی لیزین با آلانین در آنزیم می­شود که در گاو تأثیری بزرگ بر شیر و ترکیب‌های آن دارد. در این تحقیق، چندشکلی در ناحیۀ اگزون 3 تا اگزون 17 ژن DGAT1 در سه تودۀ بومی گاومیش­های ایران بررسی شد. استخراج DNA از پیاز مو با استفاده از کیت استخراج DNA از 200 رأس گاومیش (خوزستانی، شمالی و آذری) انجام گرفت و قطعه­های موردنظر از روش واکنش زنجیره­ای پلیمراز (PCR) افزایش شدند. تعیین ژنوتیپ با روش توالی­یابی مستقیم انجام شد و با نرم‌افزار BioEdit تجزیه‌وتحلیل صورت گرفت. تجزیۀ توالی‌یابی در موقعیت­های نوکلئوتیدی g.6097A>G، g.7036C>T، g.7338G>A، g.7710C>T،g.8087C>T ،g.8259G>A ،g.8275G>A  و g.8426C>T چندشکلی­های گزارش نشده‌ای (جدیدی) را در ژن DGAT1 در مقایسه با توالی موجود در بانک ژن (DQ886485) درون جمعیت گاومیش­ها نشان داده است. در مقایسه با گاومیش هندی 3 جهش در ناحیۀ اگزونی نشان داده شد که یکی از آن‌ها جهش نامترادف بود. یک جهش درج­شدگی 22 نوکلئوتیدی در اینترون 10 مشاهده شد. این نتایج گویای تنوع در توالی ژنتیکی قطعۀ بررسی‌شده در جمعیت گاومیش­های ایران است و از این تنوع می­توان در بررسی‌های ژنتیکی و اصلاح نژادی بهره جست.

کلیدواژه‌ها


عنوان مقاله [English]

Investigation of polymorphism of DGAT1 gene in Iranian buffaloes

نویسندگان [English]

  • Masoumeh Naserkheil 1
  • Seyed Reza Miraie-Ashtiani 2
  • Mostafa Sadeghi 3
  • Ardeshir Nejati-Javaremi 3
  • Deukhwan Lee 4
1 Ph.D. Student, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj
2 Professor, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 Associate Professors, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran
4 Professor, Department of Animal Biotechnology, Graduate School of Bio. & Technology, Hankyong National University
چکیده [English]

Diacylglycerol acyltransferase1 (DGAT1) is a key enzyme in synthesis of triglycerides. A transition mutation resulting substitution of guanine by adenine in DGAT1 gene and substitution of lysine by alanine in diacylglycerol-acyltransferase enzyme in cattle has a major effect on milk yield and milk composition traits. In this research, the polymorphism of the region spanning exon 3 to exon 17 of the DGAT1 gene of three indigenous Iranian buffalo populations was investigated. A total number of samples of 200 buffaloes (Khuzestani, Shomali and Azari) were collected to extract DNA and the desired fragments were amplified by polymerase chain reaction (PCR). Genotyping was performed using direct sequencing and analyzed by BioEdit software. Sequence analysis showed novel SNPs in comparative to the reference sequence GenBank (DQ886485) at nucleotide positions g.6097A>G, g.7036C>T, g.7338G>A, g.7710C>T, g.8087C>T, g.8259G>A, g.8275G>A and g.8426C>T of DGAT1 gene in buffalo populations. A comparison with Indian buffalo revealed three exonic SNPs, one of which was non-synonymous. A Unique 22 base insertion has been observed in the intron 10. The results described here genetic diversity and could be useful in genetic and breeding programs in Iranian buffalo.

کلیدواژه‌ها [English]

  • DGAT1 gene
  • Iranian buffalo
  • sequence analysis
  • SNPs
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