Filtration of home-made lecithin-based semen extender has no effect on post-thawed buck sperm quality

Document Type : Research Paper


1 Department of Poultry Sciences, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

2 Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran


The goal of this study was the evaluation of semen extender filtration on post-thawed buck sperm quality. A total 16 ejaculates were collected from four buck (4 ejaculates from each buck) twice a week. After semen collection and primary evaluation, collected semen samples were pooled and divided into two equal parts. Each part was diluted with one of the following extenders: 1) non-filtered lecithin-based extender and 2) filtered lecithin-based extender. After thawing, sperm motility and velocity parameters, plasma membrane integrity and functionality, MDA level, acrosome integrity, and apoptosis features were evaluated. The obtained results showed that all evaluated parameters, with exception of LIN, did not alter by both extenders (p > 0.05). In conclusion, it seems that filtration of lecithin-based semen extender have no detrimental effect on in vitro sperm quality parameters. However, more study is required to reveal the effect of extender filtration on advance in vitro assessments and fertility


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