Iranian Journal of animal Science

Iranian Journal of animal Science

The influence of Eucalyptus camaldulensis leaves on in vitro ruminal fermentation, protozoa population and methane production

Document Type : Research Paper

Authors
Mohammad Ebrahim Noorian Soroor 1
Yousef Roozbehan 2
1 Ph. D. Graduated, Agriculture College, Tarbiat Modares University, Tehran
2 Associate Professor, Agriculture College, Tarbiat Modares University, Tehran
10.22059/ijas.2014.54357
Abstract
The effect of three levels of eucalyptus leaf (0, 28.65 or 57.37mg/200mg substrate), which were either oven, freeze or air dried on in vitro ruminal fermentation characteristics was assessed. The studied parameters were in vitro gas production (IVGP), methane production, ammonia (NH3-N) concentration, in vitro organic matter degradability, partitioning factor (PF) (ratio of substrate truly degraded to gas volume produced at 24 h of incubation) and total volatile fatty acids (VFAs) concentration. Three subfamily of Entodiniinae, Ophryscolecinae, Diplodiniinae and family of Isotrichdaewere, also, identified and enumerated. Methods of air (L effect; P<0.01) and freeze drying (Q effect; P<0.05) have decreased the concentration of VFAs. However, oven drying method has increased propionic acid concentration (L effect; P<0.01). Adding eucalyptus leaf has significantly decreased gas production from insoluble fraction (b) (L effect; P<0.01), IVGP at 24, 54 h (L and Q effect; P<0.01), methane production (L effect; P<0.05), NH3-N concentration (L effect; P<0.01) and total VFAs (L effect; P<0.01). However, such additive has improved PF (L effect; P<0.01) only at 57.31 level. Moreover, the inclusion of eucalyptus has significantly decreased total protozoa population (P<0.01), Entoodiniinae subfamily (P<0.01) and Isotrichdae family (P<0.05). The results suggest that eucalyptus leaves has positively manipulated in vitro ruminal fermentation, and oven dried method was more effective than two other methods.
Keywords
Eucalyptus camaldulensis
gas test
methane
Ammonia nitrogen
Ruminal fermentation
sheep
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Iranian Journal of animal Science
Volume 45, Issue 4 - Serial Number 4
Winter 2015
Pages 363-374

  • Receive Date 07 May 2015
  • Revise Date 29 July 2015
  • Accept Date 14 December 2014