The effect of adding different levels of zinc sulfate to semen diluent on quality of frozen-thawed sperm in bull

Document Type : Research Paper


1 M. Sc. Student, University College of Agriculture and Natural Resources, University of Tehran, Karaj

2 Associate Professor, University College of Agriculture and Natural Resources, University of Tehran, Karaj

3 Assistant Professor, University College of Agriculture and Natural Resources, University of Tehran, Karaj


The aim of this study was to investigate the effect of adding different levels of zinc sulfate in semen extender on bovine sperm quality during cryopreservation. Semen was collected from 4 Holstein bull twice a week and the proper samples were pooled. Semen were allocated to four extender treatments consisted of 0, 50, 100, 150 µM of zinc sulfate in 5 replicates and then, were frozen. Post-thaw sperm quality including mobility, morphology, viability, membrane integrity, acrosome health, chromatin dispersion and malondialdehyde production were examined. No significant difference was observed in sperm motility and morphology in the Zinc groups, compared to control group. Viability and membrane integrity of 100µM group of zinc was better than control group (p


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