Document Type : Research Paper

Authors

1 .Sc. Student, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

2 M.Sc. Student, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

3 Assistant Professor, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

Abstract

Specific structure of ewe's cervix is one of artificial insemination limitation in sheep. Successful insemination in ewes has a close relation with morphology of their cervix. The objective of this experiment was to compare anatomical structure of cervix in Makui, Kurdish and Afshari ewes. In this study, reproductive tract of 300 ewes of aforesaid sheep (100 ewes of each strain) were collected during breeding season in slaughterhouses of Alborz and Tehran provinces. Exterior shape, opening, length and diameter of cervix, measure of penetration, kinds and number of rings were studied. The most frequent exterior shape and length were the shape of papilla and the length of 3 to 4 centimeter respectively. The dominant shape of opening in cervix in Kordi and Afshari ewes was papilla where as in Makui ewes, the dominant shape was flop. The results showed that average cervix diameter in collected respectively was 9.34±1.7. The average number of rings for all sheep between these strains was 5.59±0.8. The most frequent layout and arrangement of rings in cervical canal was the second type. The correlation between the length of cervix and number of rings, and the length and diameter of cervix were 0.24 and 0.25, respectively. There was not a significant correlation between length of cervix with ring’s angle, opening shape and age. Length of cervix, diameter and number of rings were not significantly different among 3 breeds. Average penetration of insemination gun into cervical canal for all data was 3.92±2.5 mm and were approximately 4.24± 2.2 3.46±1.1, 3.84±2.5 in Makui, Kordi and Afshari ewes, respectively. The measurement of gun’s penetration into cervix is affected by cervix diameter. Estrus cycle had a significant effect on measurement of penetration, while depth of penetration was lower during luteal phase and higher in non- luteal phase. Also the measurement of penetration was affected by number of rings, while cervixes with more number of rings resulted in less measurement of penetration. Evaluated parameters did not show any differences among breeds except the exterior shape of cervix opening.
 

Keywords

  1. Anel, L., Kaabi, M., Abroug, B., Alvarez, M., Anel, E. & Boixo, J.C. (2005). Factors influencing the success of vaginal and laparoscopic artificial insemination in churra ewes: a field assay.Theriogenology, 63, 1235-47.
  2. Ayad, VJ., Leung, ST., Parkinson, TJ. &Wathes, DC. (2004). Coincident increases in oxytocin receptor expression and EMG responsiveness to oxytocin in the ovine cervix at oestrus. Animal Reproducation Science, 80, 237-50.
  3. Bunch, T.D. & Ellsworth, H.S. (1981). Gross anatomy of the ovine cervix. Int. Goat and Sheep Research,13,282-28.
  4. Claire, M., Muhammad, Kh., Michael, R., Kate, In & Sukanya L. (2005). The anatomy of the sheep cervix and its influence on the transcervical passage of an inseminatingpipette into the uterine lumen. Theriogenology Journal, 64, 1225-1235.
  5. Dun, RB. (1955). The cervix of the ewe. Its importance in artificial insemination in sheep. Veterinaria Journal, 31,101-103.
  6. Eppleston, J., Salamon, S., Moore, NW. & Evans, G. (1994). The depth of cervical insemination and site of intrauterine insemination and their relationship to the fertility of frozen-thawed ram semen. Animal Reproducation Science, 36, 211-25.
  7. Evans, G., Maxwell, W. & Salamon, S. (1987). Artificial insemination of sheep and goats. Butterworths, 28, 1.
  8. Fair, S., Hanrahan, J.P., O'Meara, C.M., Duffy, P., Rizos, D. & Wade, M. (2005). Differences between Belclare and Suffolk ewes in fertilization rate, embryo quality and accessory sperm number after cervical or laparoscopic artificial insemination. Theriogenology, 63, 1995-2005.
  9. Fukui, Y. & Roberts, EM. (1978). Further studies on non-surgical intrauterine technique for artificial insemination in the ewe. Theriogenology, 10, 381-93.
  10. Hanrahan, JP. (2003). Aspects of reproductive performance in small ruminants opportunities and challenges. Reproduction (Cambridge, England) Supplement, 61, 15-26.
  11. Halbert, G., Dobson, H., Walton, JS. & Buckrell, BC. (1990). The structure of the cervical canal of the ewe. Theriogenology, 33, 977-92.
  12. Halbert, G., Dobson, H., Walton, J., Sharpe, P. & Buckrell, B. (1990). Field evaluation of a technique for transcervical intrauterine insemination of ewes. Theriogenology, 33, 1231-43.
  13. Kaabi, M., Alvarez, M., Anel, Ea., Chamorro C., Boixo, J.C., Paz, P. & Anel, E. (2006). Influence of breed and age on morphometry and depth of inseminating catheter penetration in the ewe cervix: A postmortem study. Theriogenology, 66, 1876-1883.
  14. Khalifa, RM., Sayre, BL. & Lewis, GS. (1992). Exogenous oxytocin dilates the cervix in ewes. Journal Animal Science, 70, 38-42.
  15. More, J. (1984). Anatomy and histology of the cervix uteri of the ewe. Acta Anatomica, 74, 156-159
  16. Naqvi, SM., Pandey, GK., Gautam, KK., Joshi, A., Geethalakshmi, V. & Mittal, JP. (2005). Evaluation of gross anatomical features of cervix of tropical sheep using cervical silicone moulds. Animal Reproducation Science, 85, 337-44.
  17. Salamon, S. & Maxwell, W. (1995). Frozen storage of ram semen I. Processing, freezing, thawing and fertility after cervical insemination. Animal Reproduction Science, 37, 185-249.
  18. Ledger, WL., Ellwood, DA. & Taylor, MJ. (1983). Cervical softening in late pregnant sheep by infusion of prostaglandin E-2 into a cervical artery. Journal of Reproduction and Infertility, 69, 511-5.
  19. Lvarez, M. (2000). Estudio del cuello uterino en la oveja Churra como me´todo de mejora de la vı´a vaginal en la inseminacio´n artificial. Tesis Doctoral. Facultad de Veterinaria, Universidad de Leo´n, Espan. 1-100.
  20. Umscheid, CA., Gordan, P. & Nathanielsz, PW. (1998). Up-regulation of oxytocin receptor messenger ribonucleic acid and protein by estradiol in the cervix of ovariectomized rat. Biology Reproducation, 59, 1131-8.