The effects of L-arginine on growth, small intestine, and immune system of broilers in starter period

Document Type : Research Paper

Authors

1 Associate professor at Faculty of Veterinary Medicine, University of Tehran

2 Assistant professor at Faculty of Agricultural Science, University of Tabriz-Tabriz

3 Professors, University College of Agriculture & Natural Resources, University of Tehran-Karaj

4 Assistant professor at Sari Agricultural Sciences & Natural Resources University

5 Assistant professor at Razi Vaccine and Serum Research Institute-Karaj

6 Associate Professor at School of Public Health, Tehran University of Medical Sciences

Abstract

The objective of the present study was to investigate the effects of high dietary levels of L-arginine during starter period on growth, intestine morphology and immune system of female Ross broiler chicks. In this experiment, 192 day old female Ross broiler chicks were fed with 4 dietary treatments in a completely randomized design, in which each dietary treatment included 4 replications. Dietary treatments included 100%, 153%, 168% and 183% of digestible arginine, based on the Ross recommendation, and were fed from day 1 to 10. On d 10 of the experiment, chicks were weighed and feed consumption was recorded. Then, three chicks per replication were slaughtered in order to survey intestinal histology and immune system. The results showed that high dietary levels of arginine had an increasing effect (P<0.01) on 10th day body weight, feed efficiency, immune system characteristics, weight and length of small intestine, and its villus height and crypt depth. According to the results of this study, 168% digestible arginine, based on the Ross recommendation, had the best desirable results on growth improvement of broiler chicks and it also improved weight, length and histology of small intestine, and immune characteristics.
 

Keywords

References

  1. Al-Daraji, H. J. & Salih, A. M. (2012). Effect of dietary L-arginine on carcass traits of broilers. Research Opinions in Animal and Veterinary Science, 2, 40-44.
  2. Andrews, R.P. & Baldar, N.A. (1985). Amino acid analysis of feed constituents. Science Tools, 32, 44-48.
  3. AOAC. (2000). Official methods of analysis. Association of Official Analytical Chemists. EUA.
  4. Ball, R. O., Urschel, K. L. & Pencharz, P. B. (2007). Nutritional consequences of interspecies differences in arginine and lysine metabolism. Journal of Nutrition, 137, 1626–1641.
  5. Bauchart-Thevret, C., Cui, L., Wu, G. & Burrin, D. G. (2010). Arginine-induced stimulation of protein synthesis and survival in IPEC-J2 cells is mediated by mTOR but not nitric oxide. American Journal of Physiology: Endocrinolology and Metabolism, 299, E899-909.
  6. Bedford, M. (2000). Removal of antibiotic growth promoters from poultry diets: Implications and strategies to minimize subsequent problems. World Poultry Science Journal, 56, 347-365.
  7. Caspary, W.F. (1992). Physiology and pathophysiology of intestinal absorption. The American Journal of Clinical Nutrition, 55, 299S-308S.
  8. Corrier, D. E. & DeLoach, J. R. (1990). Evaluation of cell-mediated, cutaneous basophil hypersensivity in young chickens by an interdigital skin test. Poultry Science, 69, 403–408.
  9. Currie, G. A. (1978.) Activated macrophages kill tumour cells by releasing arginase. Nature, 273, 758.
  10. De Ridder K., Levesque C.L., Htoo J.K. & De Lange C.F. (2012) Immune system stimulation reduces the efficiency of tryptophan utilization for body protein deposition in growing pigs. Journal of Animal Science, 90, 3485-3491.
  11. Fernandes, J. I. M., Murakami, A. E., Martins, E. N., Sakamoto, M. I. & Garcia, E. R. M. (2009). Effect of arginine on the development of the pectoralis muscle and the diameter and the protein: deoxyribonucleic acid rate of its skeletal myofibers in broilers. Poultry Science, 88, 1399–1406.
  12. Floyd, J. C. J., Fajans, S. S. & Conn, J. W. (1966). Stimulation of insulin secretion by amino acids. Journal of Clinical Investigation, 45, 1487–1502.
  13. Jahanian, R. (2009). Immunological responses as affected by dietary protein and arginine concentrations in starting broiler chicks. Poultry Science, 88, 1818–1824.
  14. Jiao, P., Guo, Y., Yang, X. & Long, F. (2010). Effect of dietary arginine and methionine levels on broiler carcass traits and meat quality. Journal of Animal and Veterinary Advances, 9, 1546-1551.
  15. Jobgen, W. S., Fried, S. K., Fu, W. J., Meininger, C. J. & Wu, G. (2006). Regulatory role for the arginine–nitric oxide pathway in metabolism of energy substrates. The Journal of Nutritional Biochemistry. 17, 571-588.
  16. Khajali, F. & Widerman, R. F. (2010). Dietary arginine: metabolic, environmental, immunological and physiological interrelationships. World's Poultry Science Journal, 66, 751-766.
  17. Kidd, M. T., Peebles, E. D., Whitmarsh, S. K., Yeatman, J. B. & Wideman, R. F. (2001). Growth and immunity of broiler chicks as affected by dietary arginine. Poultry Science, 80, 1535–1542.
  18. Klein, D. & Morris, D. R. (1978). Increased arginase activity during lymphocyte mitogenesis. Biochemical Biophysical Research Communications, 81, 199.
  19. Kwak, H., Austic, R. E. & Dietert, R. R. (1999). Influence of dietary arginine concentration on lymphoid organ growth in chickens. Poultry Science, 78, 1536-1541.
  20. Kwak, H., Austic, R. E. & Dietert, R. R. (2001). Arginine-genotype interactions and immune status. Nutrition Research, 21, 1035–1044.
  21. Law, G. K., Bertolo,  R. F., Adjiri-Awere,  A., Pencharz,  P. B. & Ball,  R. O. (2007). Adequate oral threonine is critical for mucin production and gut function in neonatal piglets. American Journal of Physiology- Gastrointestinal and Liver Physiology, 292, G1293-G1301.
  22. Munir, K., Muneer, M. A., Masaoud, E., Tiwari, A., Mahmud, A., Chaudhry, R. M. & Rashid, A. (2009). Dietary arginine stimulates humoral and cell-mediated immunity in chickens vaccinated and challenged against hydropericardium syndrome virus. Poultry Science, 88, 1629–1638.
  23. Murakami, A. E., Fernandes, J. I. M., Hernandes, L. & Santos, T. C. (2012). Effects of starter diet supplementation with arginine on broiler production performance and on small intestine morphometry. Pesquisa Vet Brasil, 32(3), 259-266.
  24. Nall, J. L., Wu, G., Kim, K. H., Choi, C. W. & Smith, S. B. (2009). Dietary supplementation of L-arginine and conjugated linoleic acid reduces retroperitoneal fat mass and increases lean body mass in rats. Journal of Nutrition, 139, 1279-1285.
  25. Navidshad, B., Adibmoradi, M. & Ansari Pirsaraei, Z. (2010). Effects of dietary supplementation of Aspergillus originated prebiotic (Fermacto) on performance and small intestinal morphology of broiler chickens fed diluted diets. Italian Journal of Animal Science, doi: 10.4081/ijas.2010.e12.
  26. NRC. (1994). Nutrient requirements of poultry, 9th ed. (Washington, DC, National Academy Press).
  27. Pluske, J. R., Hampson, D. J. & Williams, I. H. (1997). Factors influencing the structure and function of the small intestine in the weaned pig – a review. Livestock Production Science, 51, 215- 236.
  28. Poosti, A. & Adibmoradi, M. (2008). Laboratory technics in histology. University of Tehran Press. (In Farsi).
  29. Qureshi, M. A. (2003). Avian Macrophage and Immune Response: An Overview. Poultry Science 82, 691-698.
  30. Rodríguez, P. C. & Ochoa, A. C. (2008). Arginine availability regulates T-cell function in cancer. D. I. Gabrilovich, A. A. Hurwitz (eds.), Tumor-Induced Immune Suppression. Springer, 219-233.
  31. Tan, B., Yin, Y., Kong, X., Li, P., Li, X., Gao, H., Li, X., Huang, R. & Wu, G. (2010). L-Arginine stimulates proliferation and prevents endotoxin-induced death of intestinal cells. Amino Acids, 38, 1227–1235.
  32. Tayade, C., Koti, M. & Mishra, S. C. (2006). L-arginine stimulates intestinal intraepithelial lymphocyte functions and immune response in chickens orally immunized with live intermediate plus strain of infectious bursal disease vaccine. Vaccine, 24, 5473– 5480.
  33. Uni, Z. (1999). Functional development of the small intestine in domestic birds: Cellular and molecular aspects. Poultry and Avian Biology Review, 10,167–179.
  34. Uni, Z. & Ferket, P. R. (2004). Methods for early nutrition and their potential. World’s Poultry Science J. 60,101–111.
  35. Uni Z., Tako E., Gal-Garber, O. & Sklan, D. (2003). Morphological, molecular, and functional changes in the chicken small intestine of the late-term embryo. Poultry Science, 82, 1747–1754.
  36. Visek, W.J. (1978). The mode of growth promotion by antibiotics. J. Anim. Sci, 46, 1447-1469.
  37. Wang, X., Qiao, S., Yin, Y., Yue, L., Wang, Z. & Wu, G. (2007). Deficiency or excess of dietary threonine reduces protein synthesis in jejunum and skeletal muscle of young pigs. Journal of Nutrition, 137, 1442-1446.
  38. Wu, X., Ruan, Z., Gao, Y., Yin, Y., Zhou, X., Wang, L., Geng, M., Hou, Y. & Wu, G. (2010). Dietary supplementation with L-arginine or N-carbamyl glutamate enhances intestinal growth and heat shock protein-70 expression in weanling pigs fed a corn- and soybean meal-based diet. Amino Acids, 39, 831-839.
  39. Yamauchi, K., Kamisoyama, H. & Isshiki, Y. (1996). Effects of fasting and refeeding on structures of the intestinal villi and epithelial cells in White Leghorn hens. British Poultry Science, 37, 909– 921.
  40. Yao, K., Guan, S., Li, T., Huang, R., Wu, G., Ruan, Z. & Yin, Y. (2011). Dietary L-arginine supplementation enhances intestinal development and expression of vascular endothelial growth factor in weanling piglets. British Journal of Nutrition, 105, 703–709.
  41. 41.              Yason, C.V., Summers, B. A. & Schat, K. A. (1987). Pathogenesis of rotavirus infection in various age groups of chickens and turkeys: pathology. American Journal of Veterinary Research, 48, 927-938.
Iranian Journal of animal Science
Volume 45, Issue 3 - Serial Number 3
October 2014
Pages 223-233

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History

  • Receive Date: 27 October 2013
  • Revise Date: 25 July 2014
  • Accept Date: 05 August 2014

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