The objective of this study was to determine the effects of hulled and hull-less barley grains’ chemical treatment on disappearance of dry matter (DM), starch and CNCPS carbohydrate fractions. For barley grain treatment, NaOH, formaldehyde and urea were used at 35, 4 and 35 gr/kg DM respectively. Three mature rams (one year old) of Zel breed were rumen cannulated to calculate ruminal disappearance (percent) of DM and starch. Incubation time of nylon bags were 0, 2, 4, 6, 8, 12, 24 and 48 hours. To evaluate CNCPS carbohydrate fraction characteristics, its parameters were measured. Rumen content disappearance results indicated that NaO¬¬¬¬H and formaldehyde treatment had protective effect on DM and starch from rumen digestion leading to better results rather than urea treatment and control groups. Also, starch disappearance of hulled barley was lower than hull-less barley (P<0.05). Chemical treatment of barley grains significantly affected CNCPS carbohydrate fraction characteristics. Each chemical treatment method (in comparison to control) had significant decreasing effects on high speed degradation of such fractions as A and B1 (P<0.05). C fraction or indigestible fiber in hulled barley (with no treatment) was maximum (1.00%) exhibiting had significant difference with those treated with formaldehyde and urea (P<0.05). Therefore based upon the obtained results, rumen content disappearance showed its most effectiveness of NaOH and formaldehyde groups rather than urea and control, but CNCPS fractions were not significantly affected by different chemical processings.