The Investigation of some Dinucleotide Microsatellite Loci in Japanese Quail (Coturnix Japonica)

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Abstract

Proliferation trend and polymorphism in 8 microsatellite dinucleotidalloci, a characteristic of Japanese quail, were described in the present study. The primers concerned were tested in 200 birds from Bonab Research Station. Samples were taken from four strains of Pharach, Golden, Tuxedo, and Panda. In total, all loci were polymorphic, except for GUJ0001 and GUJ0041 in respective strains of Panda and Tuxedo which were monomorphic. Various interpopulation variability parameters were studied for the tudy population. The average heterozygosity for all loci was 0.366. Furthermore the average rate of heterozygosity, without an inclusion of monomorphic loci, was estimated as 0.374. Maximum and minimum polymorphism Information content (PIC) and Shannon Information Index (I) were respectively obtained for GUJ0059 and GUJ0001 loci. Results indicate that in spite of selection for successive years along with closed mating system, there still exists a rather high level of polymorphism in the study population.

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