Iranian Journal of animal Science

Iranian Journal of animal Science

The Investigation of some Dinucleotide Microsatellite Loci in Japanese Quail (Coturnix Japonica)

Authors
mohammad ali rajaii arbabi
hossein omrani
siroos amiri nia
rasoul vaez torshizi
ardeshir nejati javaremi
saied esmail khanian
amir mir hadi
Abstract
Proliferation trend and polymorphism in 8 microsatellite dinucleotidalloci, a characteristic of Japanese quail, were described in the present study. The primers concerned were tested in 200 birds from Bonab Research Station. Samples were taken from four strains of Pharach, Golden, Tuxedo, and Panda. In total, all loci were polymorphic, except for GUJ0001 and GUJ0041 in respective strains of Panda and Tuxedo which were monomorphic. Various interpopulation variability parameters were studied for the tudy population. The average heterozygosity for all loci was 0.366. Furthermore the average rate of heterozygosity, without an inclusion of monomorphic loci, was estimated as 0.374. Maximum and minimum polymorphism Information content (PIC) and Shannon Information Index (I) were respectively obtained for GUJ0059 and GUJ0001 loci. Results indicate that in spite of selection for successive years along with closed mating system, there still exists a rather high level of polymorphism in the study population.
Keywords
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heterozygosity
Japanese quail
Microsatellite
Polymorphism