Determination of nutritional value, different protein fractions of black soldier fly larvae in the Net Carbohydrate and Protein System and degradability by nylon bag method

Document Type : Research Paper

Authors

1 Animal Science Department,,Faculty of Science and agricultural engineering, Razi University, Kermanshah, Iran

2 Animal science Department, Faculty of Science and Agricultural engineering, Razi university, Kermanshah, Iran.

3 Department of Animal Science, Faculty of Agriculture, Bu-Sina University, Hamadan, Iran

4 Animal Science Department,, Faculty of Agriculture, Bu-Ali Sina University, Hamadan, Iran

Abstract

This research focused on evaluating black soldier fly larvae as a potential alternative protein source for animal feed by analyzing their protein fractions using the Cornell Net Carbohydrate and Protein System and assessing protein degradability with the nylon bag method. Upon completion of the larval growth phase, their approximate composition, fatty acid, and amino acid profiles were analyzed. The rearing process showed that 85 grams of eggs produced 63.5 kilograms of dried larvae, and 225.76 grams of fresh larvae were generated per kilogram of feed consumed. The entire growth and hatching period lasted 15 days, with larvae reaching an average size of two centimeters. The larvae contained 41.71% crude protein and 35.01% ether extract, with high levels of essential amino acids such as methionine and lysine, and non-essential amino acids including aspartic and glutamic acids. The main fatty acids were saturated lauric and palmitic acids, along with unsaturated oleic and linoleic acids. Medium-degradable true protein was the largest fraction, while non-protein nitrogen and rapidly degradable true protein made up 14.58% and 9.63% of the protein, respectively. The potential and effective degradability of crude protein at a passage rate of 0.05 were 43.97% and 31.59%, respectively. Overall, the results suggest that black soldier fly larvae possess favorable nutritional qualities and efficiently convert organic waste into valuable nutrients, making them a viable alternative protein source for ruminant feeding.

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Articles in Press, Accepted Manuscript
Available Online from 31 August 2025
  • Receive Date: 15 July 2025
  • Revise Date: 21 August 2025
  • Accept Date: 26 August 2025